ISfinder Mirror Site

ISCal1

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP002453	ND	Cellulophaga algicola	Cellulophaga algicola DSM 14237

DNA section

IS Length : 959 bp

Ends

IR Length : 21/25

IRL : TAGAGGTCAGTAGCTAACTTCGTATCTTGATACTCAGTAAATATATTAAA
IRR : TAGAGGTCAGTAGTTAATTACTTATTAATTCAGCTTGATTGATTTTATCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAATTTAAAT	TAATGTTT	TTCTAATCAT	8
GCAAATGAAT	AAAAATTT	TTTAAAGTTT	8
CAAATAGAAT	TAATATTA	TTTACTTCTT	8
CATTCCCTTT	TAAAGTTT	TATAATATTC	8
ACACTAAATA	TAAAGTAG	ATTAAAAGTT	8
CCTAGACATA	TAAATATT	AAAGCGTTAG	8
TAAAGTAGAT	AAATTTTA	TTAAATCGCA	8
GCTTGTAATA	AAAGTATT	TAATAAAATT	8
ATAATAGAAA	TAAACGGA	AATAAATTTT	8
TGTTTTAATA	TTCCGCTA	ATTGTTTTTC	8
TTATTTATTT	AAACGTTA	TTTAATAACT	8
TAATTATATA	TTAAATTT	AATTGCTTAG	8
ATTGTACTTT	TAATTCTT	TATTATTTGG	8
GAAAGTAAAT	TAAATTTA	TATCAAACTC	8
TAATGGCAAA	AAAAAATA	TATAAAAACT	8
AAATCACTTT	AAACCTTA	TTTTAAAACC	8
ACCTTGGATA	TAAGAAAA	ATTAAACTAC	8
GTTTACTATT	TAAGGTTT	AATTAATTCA	8
ACACTTAAAT	AAAAATTT	AACGATTTTA	8
TTTGTAAATA	AAAAATAA	AATGCGGACG	8

DNA sequence

TAGAGGTCAGTAGCTAACTTCGTATCTTGATACTCAGTAAATATATTAAATTATGGATATTTTCAAGGGTCAAAATCTTCTAGAGTTCTCTGATTGCTTC
AAAACGGACAATGATTGCAAAGAATATTTAGCAAATATTAAGTCTAAAACCCCTTTTAAATGTTCTAGATGCAATCATATAGCCTGTCAAACACGTGCTG
ATTTCTCTAGGCAATGTAATATTTGTAGACATACAGAATCCGCAACAGCAGATACATTATTTCACAAGGTAAAGTTTGGTGTTCGCAAAGCATTTTTTAT
TTGTTTTGAGATGGCTACAAGCACGAAAAGCTTATCTGCAAGTTATATGGGAGTACGTTACGGAGTAACAGAAAAAACAGCTAGACTTTTTATGCTTAAG
GTCAGAGAAGCTATGTCTTCGAGTGGGAATAATCCTATGGACGGAGTTGTTCATGTAGATGAATTTGTTTTAGGGGGCAGAGAAGAAACAAAAGTTGGCA
GAAGCTACAATGCTAAGAAAAAGAAGGCGGTTACAGCTGTTCAGCTTACAGAAGATGGAAAGGTAAAAAGAATGTATGCTATGAAAATAGATGATTTTTC
AGCACAATCCTTACAATATATTTTTGTCAACCATATCAGCCGAAACGCAAAGATTACTACAGATAAATGGAGAGGCTATAGTCCTATTGCAAAGGCTTAC
GACATCACACAAATAGAAAGTAATGGAGGGTTAAATTTTAAAGCGCTTCATACAATGATACATCAGGTTAAATCTTGGATAAGAACAACTTATTCTTGGG
TTAGTGACAATAATTTAAATAGATATTTCAATGAATTTTGTTTTAGAATAAACAGATCTCAAAGTAAAGCTACAATATTCAATAATCTTATTGTTAAAAT
GGTCAATAATGATAAAATCAATCAAGCTGAATTAATAAGTAATTAACTACTGACCTCTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
894 bp	297 aa	53	946	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDIFKGQNLLEFSDCFKTDNDCKEYLANIKSKTPFKCSRCNHIACQTRADFSRQCNICRHTESATADTLFHKVKFGVRKAFFICFEMATSTKSLSASYMG
VRYGVTEKTARLFMLKVREAMSSSGNNPMDGVVHVDEFVLGGREETKVGRSYNAKKKKAVTAVQLTEDGKVKRMYAMKIDDFSAQSLQYIFVNHISRNAK
ITTDKWRGYSPIAKAYDITQIESNGGLNFKALHTMIHQVKSWIRTTYSWVSDNNLNRYFNEFCFRINRSQSKATIFNNLIVKMVNNDKINQAELISN

Blast result :

Comments

ISCal1 is 93% aa similar to ISAlg1.

References

1] ISfinder annotation (2016)
2] Abt,B., Lu,M., Misra,M., Han,C., Nolan,M., Lucas,S., Hammon,N., Deshpande,S., Cheng,J.F., Tapia,R., Goodwin,L., Pitluck,S., Liolios,K., Pagani,I., Ivanova,N., Mavromatis,K., Ovchinikova,G., Pati,A., Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D., Detter,J.C., Brambilla,E., Rohde,M., Tindall,B.J., Goker,M., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lapidus,A. (2011) Stand Genomic Sci 4 (1), 72-80.