ISfinder Mirror Site

ISBaba2

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
MENH01000158	ND	Bacteroidetes bacterium	Bacteroidetes bacterium GWA2_40_14

DNA section

IS Length : 969 bp

Ends

IR Length : 21/25

IRL : TAGAGGTGAGTAGATAAATACATATTTTCATTATCTTTGAGTCCAATAAA
IRR : TAGAGGTAGGTAGTTAACTACATATCAATTTTGCTTGATAGATTTTGTCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTGGGACTTA	TAATTGTT	AATGTAACTA	8

DNA sequence

TAGAGGTGAGTAGATAAATACATATTTTCATTATCTTTGAGTCCAATAAAATCAATGGCTGTATGAATTTATTTCAAGGACAAGACCTCTTAGAGTTTGC
TGAACGCTTTAAAACCGATGAAGACTGCATGGAATACCTGGCCCATTTTAAATGGGAGGACGGGTTCACGTGTACAAAGTGCGGCCATACGGGCAGCCAA
GCAAGAAAGGACCATTCAAGGACTTGCAACAAGTGCAGCCATACCGAATCGGCAACGGCAAACACCCTGTTCCATAAAGTGAAGTTTGGGGTGCGCAAGG
CATTCTTTATTTGCTTTGAGATGGCAACAACAACCAAGAGTTTATCGGCCAGCTATGTTGCGGAACGCTTTAGTGTGACAACAAAGACGGGAAGGCTGTT
CATGCACAAAGTCCGCGAGGCCATGAAGTCCAGTGGCAAACATCCCCTAAAGGGCAAGGTCCATATAGATGAATTTGTCATAGGCGGCAAAGAAGAAGGG
AAAGTCGGCCGCAGTTATGACAGCAAAAAAAAGAAAGTAGTGTGTGCGGTTGAGCTTACAGATGAAGGGAAAGTCAAGCGTATGTACGCAATGGGGATTG
ACAACTATTCTACCAAAGAGTTGAAAAAGCTGTTTGAGAAACATATTTCCGGGGAAGCAGAAGTAACCACGGACTGTTGGAAAGGTTACCGTCCTTTAAT
GGCGGAGTACAATATCGTTCAGGTTGAAAGCAGCGGCGGTCTGAACTTTAAAGCCCTACATACAATGATACACCAGGTAAAATCATGGATAAGGACAACT
TATTCCTGGGTGAGCGAGTTCAACATCAATCGGTATCTTGACGAATTTTGCTTCAGGTTAAACCGCTCGCAGATGAAAAACAATGTATTCAACAATATTA
TTGGCAGGATGGTGGCGGCTGACAAAATCTATCAAGCAAAATTGATATGTAGTTAACTACCTACCTCTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
894 bp	297 aa	63	956	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNLFQGQDLLEFAERFKTDEDCMEYLAHFKWEDGFTCTKCGHTGSQARKDHSRTCNKCSHTESATANTLFHKVKFGVRKAFFICFEMATTTKSLSASYVA
ERFSVTTKTGRLFMHKVREAMKSSGKHPLKGKVHIDEFVIGGKEEGKVGRSYDSKKKKVVCAVELTDEGKVKRMYAMGIDNYSTKELKKLFEKHISGEAE
VTTDCWKGYRPLMAEYNIVQVESSGGLNFKALHTMIHQVKSWIRTTYSWVSEFNINRYLDEFCFRLNRSQMKNNVFNNIIGRMVAADKIYQAKLICS

Blast result :

Comments

ISBaba2 is 90% aa similar to ISUnb2.

References

1] ISfinder annotation (2016)
2] Anantharaman,K., Brown,C.T., Hug,L.A., Sharon,I., Castelle,C.J., Probst,A.J., Thomas,B.C., Singh,A., Wilkins,M.J., Karaoz,U., Brodie,E.L., Williams,K.H., Hubbard,S.S. and Banfield,J.F. (2016) Nat Commun 7, 13219.