ISPpu27

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP005976 ND Pseudomonas putida
Pseudomonas putida H8234
Pseudomonas mandelii LMG 21607
DNA section
IS Length : 2581 bp

Ends


IR Length : 23/27

IRL : tgttgccgctgaccgaaaactgacccagtaaaggctgttctgccgactga
IRR : tgtcggcgctgattgaaaactgacccaccctgccgattgaaaactgaccc

Insertion site


Left flankDirect repeatRight flankDR Length
GAAGAAGTGCCGATAGCATCTAGA4

DNA sequence

TGTTGCCGCTGACCGAAAACTGACCCAGTAAAGGCTGTTCTGCCGACTGAAAACTGACCCAGGTGTTCAACTGCTTCTGCTCACTTTTCGAGCAGGAGAA
CACAGGGTGATCAGCATGGAAATGTTGGGAAAAATCCGGCGGATGTACTTCCGCGACAAGCTCTCGCTGCATCAGATAGCCAAGCGTACCGGACTGTCGA
GAAACACCATTCGAAAATGGGTCAGAGCACCCGAAGCCACCCAGCCGGCGTACCAACGGTGCGCAACCTTCAACAAACTCAGTCCATTTCACGAGACGCT
GGAGCAGGCGCTTAAGGCCGATTCGTTTCGGGCAAAGCACAACCGCAGGAGTGCCAAAGCACTCTTCGAACAGATCAAGGCCGAGGGGTACGACGGCGGC
TACAGCCAACTCACCGCCTTTATACGCTCGTGGCGCGGCGAGCAAGGCAAGTCTTTACGAGCCTTTGTGCCGCTGACCTTTGCACTCGGGGAGGCCTTTC
AATTCGACTGGAGCGAAGAAGGTCTGCTGATCGGCGGCCTGTTCCGACGCATCCAGGTCTCCCATATGAAGCTGTGCGCCAGTCGCGCATTTTGGTTGGT
TGCGTATCCCAGCCAAGGCCACGAGATGTTGTTCGATGCCCATACCCGCTCGTTTGGCGCGTTAGGTGGGGTGCCGCGTCGCGGCATCTACGACAACATG
AAGACCGCTGTCGACAAGGTCAACAAGGGTAAGGGCCGCACGGTCAATGCTCGGTTTGCCGTGATGTGTGCGCACTACCTGTTCGACCCGGACTTCTGCA
ACGTCGCTTCCGGCTGGGAAAAAGGCATCGTTGAAAAGAACGTCCAAGACAGCCGACGGCGCATCTGGCTCGATGCTCAAAACTGCATGTTCCACACCTT
CGAGGAATTGAATGCCTGGCTCGGCCAACGCTGCCGTGCGCTCTGGGGCGAACTGCTGCACCCGCAGTACAACGGGCTGACCGTGGCGGACGTCCTGGAG
CTGGAACGCGCTGAATTGATGCCGATGCCGACTGCCTTTGATGGCTACGTCGAGCGCACCGTACGGGTCTCCAGCACCTGCCTAATCAGCGTGGCGCGAA
ATCGCTATTCGGTGCCGTGTGAGCGGGTCGGCCAGTGGGTCAGCAGCCGTTTGTATCCTTCCCGGGTGGTGGTCATCGCCGATGAAACGACGATTGCCAG
CCACGAGCGTCTGTTTGATCGAGATCAGGTCAGTTTTGACTGGCAGCATTACATCCCGCTTATCGAGCGCAAGCCTGGAGCGCTGCGCAATGGCGCTCCG
TTTGCTGATCTGCCGAAGCCGTTGCAGCTGCTAAAACGCGGTCTGAGGCGTCACGCCAATGGTGATCGAGTCATGACGCAGGTACTGGCGGCTGTTCCCA
TCGCCGGGCTCGACGCCATATTGGTGGCTGTGGAGTTGGTACTTGAATCAGGCAGCTTGAGTGCTGAGCACATCCTGAATGTCCTGGCTCGACTGACATC
GACAGCAGCCCCTCCGTCCGCCGAAACCAGCCTTCAACTCAAGATAGCGCCTGTCGCCAATACGGCACGCTACGACCGGCTCCGTACCACCGATGAGGAG
ACTCGCAATGCGTGATCTTATGGCGGAACTCAAAGAACTGCGTCTGCATGGCATGGCCAGCGCCTGGGAAGAACTAGCCTCGCAAGGAGAAGCTTCGACG
GCATCCTCGAAATGGCTACTTGAGCATCTTCTCCAACAAGAACATGCGGATCGAGCGGTGCGCTCAGTGAACCACCAGATGAACATGGCCAAACTCCCCA
TGCATCGTGATTTGGCCGGCTTCGACTTCAGCGCTTCCAGCGCAGATGCCCGCCTGATCAGGGATCTGTCCAACCTGGAGTTCACCGATACCGCGCAGAA
TGTCGTGTTCATCGGCGGGCCTGGAACAGGCAAAACACACCTCGCCACCGCCTTGGCTGTGTCCGGGATCACGACGCATCACAAACGCGTGCGCTTCTAC
TCCACGGTGGATCTGGTCAATCTGCTGGAACGCGAAAAGTACGACGGCAAGACGGGCCGCATCGCTCAAGGGCTGCTACGAACAGACCTGGTGATACTCG
ATGAACTGGGGTATTTGCCCTTCAGCCAGAGCGGTGGTGCCCTGTTATTTCACCTGCTATCCAAGCTGTACGAGCACACCAGCGTGGTGATCACCACCAA
CCTCAGCTTCTCGGAATGGTCGAGCGTGTTTGGTGACGCCAAGATGACAACGGCGTTGCTGGATCGGCTGACACACCACTGCCACATCGTCGAAACGGGC
AACGAGTCCTACCGCCTGCAACACAGCAGCTTGGTCGCCCAGACCAAGATCAAATCACGTGAACGAAAGCGCAAAGGCAATGATGGTGCCGAGGACGATG
AGCTGTTTTGATCTGCACCAAAAATACCCTGCCGCATGCCCACATGCGGTGGGGCTTTAATGCCTCTTCCACCGGGACACGACTGCTAAGCTTATCCACA
ATTGCTGATGCCAAAATCAGCAATCCGCCCTGGGTCAGTTTTCAATCGGCAGGGTGGGTCAGTTTTCAATCAGCGCCGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1509 bp502 aa1071615+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISMEMLGKIRRMYFRDKLSLHQIAKRTGLSRNTIRKWVRAPEATQPAYQRCATFNKLSPFHETLEQALKADSFRAKHNRRSAKALFEQIKAEGYDGGYS
QLTAFIRSWRGEQGKSLRAFVPLTFALGEAFQFDWSEEGLLIGGLFRRIQVSHMKLCASRAFWLVAYPSQGHEMLFDAHTRSFGALGGVPRRGIYDNMKT
AVDKVNKGKGRTVNARFAVMCAHYLFDPDFCNVASGWEKGIVEKNVQDSRRRIWLDAQNCMFHTFEELNAWLGQRCRALWGELLHPQYNGLTVADVLELE
RAELMPMPTAFDGYVERTVRVSSTCLISVARNRYSVPCERVGQWVSSRLYPSRVVVIADETTIASHERLFDRDQVSFDWQHYIPLIERKPGALRNGAPFA
DLPKPLQLLKRGLRRHANGDRVMTQVLAAVPIAGLDAILVAVELVLESGSLSAEHILNVLARLTSTAAPPSAETSLQLKIAPVANTARYDRLRTTDEETR
NA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
804 bp267 aa16082411+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRDLMAELKELRLHGMASAWEELASQGEASTASSKWLLEHLLQQEHADRAVRSVNHQMNMAKLPMHRDLAGFDFSASSADARLIRDLSNLEFTDTAQNVV
FIGGPGTGKTHLATALAVSGITTHHKRVRFYSTVDLVNLLEREKYDGKTGRIAQGLLRTDLVILDELGYLPFSQSGGALLFHLLSKLYEHTSVVITTNLS
FSEWSSVFGDAKMTTALLDRLTHHCHIVETGNESYRLQHSSLVAQTKIKSRERKRKGNDGAEDDELF

 

Blast result :
Comments
ISPpu27 is 97% (transposase) and 95% (IS21 helper) aa similar to ISPsy20.
References