ISPeat2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP009125 | ND | Pectobacterium atrosepticum | Pectobacterium atrosepticum 21A Pectobacterium atrosepticum JG10-08 Pectobacterium atrosepticum Pectobacterium atrosepticum SCRI 1043 |
DNA section
IS Length : 1244 bp
Ends
IR Length : 27/35
IRL : TGATCCTCCCCAACAATAGTGGACACGCAACTAAGTGAGTAAACTCTCAA
IRR : TGATCCTACCCACCTAATGTGGACACGGCCCTAAGCGAGGTTCTGGTTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTAAACAGCT | GTG | TACCAATAGA | 3 |
DNA sequence
TGATCCTCCCCAACAATAGTGGACACGCAACTAAGTGAGTAAACTCTCAAGTAAGAGGTGACTCACATGACAAAATCAGTATCAACCAAGAAGCCCCGTA
AACAGCATACGCCAGAGTTTCGCGATGAAGCCCTAAAATTGGCTGGACGCATCGGTGTTGCTGCCGCAGCCCGTGAACTCAGCCTGTACGAGTCACAGCT
TTATGCCTGGCGCAGTAAACAGCAGCAACAAATGACTTCTTCAGAGCGTGAGAGCGAGCTTGCCGTTGAAAATGCCCGCCTTAAGCGACAACTGGCTGAG
CGGGATGAGGAGTTGGCCATTCTCCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTTATCGAAAAACATCGGGCTGAATTCAC
TGTCAGGACCATGTGTCGTATGCTTCGGGTTGCCCGCAGCGGCTGGTATGCCTGGCGAGTGCGCCGCCATCAGATAAGCCCGCGTCAGCAGTTCCGTCTC
ACTTGCGATGGCGCGGTCCGTAAGGCATTCGGTGACGCAAAACAGCGCTATGGTGCGCCACGTCTTGCTGATGAGCTTCCGGCCTACAACATCAAAACCA
TTGCCGCCAGCCTGCGTCGTCAGGGACTACGAGCGAAAGCGGCTCGGAAGTTCAGCCCGGTCAGCTACCGTGAACATGGGTTGCCAGTATCAGAGAACCT
ATTGAAGCAGGATTTTTACGCCAGCGGCCCGAATCAAAAATGGGCCGGCGATTTCACGTATTTACGCACGGATGAAGGGTGGCTTTATCTTGCCGTTGTT
CTCGACCTGTGGTCGCGTGCAGTTATAGGCTGGTCGATGTCATCGCGAATGACGGCGGCGCTGGCATGTAATGCACTGCAAATGGCACTGTGGAGGCGAA
AGCGTCCACAAAACGTTATTGTACATACCGATCGCGGTGGTCAGTATTGTTCAGCGGATTATCAAGCGATGCTGAATGAGCATAATCTGCGTGGCAGTAT
GAGTGCGAAGGGATGTTGTTACGACAATGCCTGCGCGGAAAGCTTCTTTCACTCGCTAAAAGTAGAATGTATCCACGGCGAACGCTTTGTCAGCAGGGAA
ATAATGCGAACAGCGGTGTTTAATTATATCGAGTGTGATTACAATCGCTGGCGTCGTCACAGTGCCTGTGGCGGACTCAGCCCGGAACAATTTGAAAACC
AGAACCTCGCTTAGGGCCGTGTCCACATTAGGTGGGTAGGATCA
AACAGCATACGCCAGAGTTTCGCGATGAAGCCCTAAAATTGGCTGGACGCATCGGTGTTGCTGCCGCAGCCCGTGAACTCAGCCTGTACGAGTCACAGCT
TTATGCCTGGCGCAGTAAACAGCAGCAACAAATGACTTCTTCAGAGCGTGAGAGCGAGCTTGCCGTTGAAAATGCCCGCCTTAAGCGACAACTGGCTGAG
CGGGATGAGGAGTTGGCCATTCTCCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGAAGTATGTCTTTATCGAAAAACATCGGGCTGAATTCAC
TGTCAGGACCATGTGTCGTATGCTTCGGGTTGCCCGCAGCGGCTGGTATGCCTGGCGAGTGCGCCGCCATCAGATAAGCCCGCGTCAGCAGTTCCGTCTC
ACTTGCGATGGCGCGGTCCGTAAGGCATTCGGTGACGCAAAACAGCGCTATGGTGCGCCACGTCTTGCTGATGAGCTTCCGGCCTACAACATCAAAACCA
TTGCCGCCAGCCTGCGTCGTCAGGGACTACGAGCGAAAGCGGCTCGGAAGTTCAGCCCGGTCAGCTACCGTGAACATGGGTTGCCAGTATCAGAGAACCT
ATTGAAGCAGGATTTTTACGCCAGCGGCCCGAATCAAAAATGGGCCGGCGATTTCACGTATTTACGCACGGATGAAGGGTGGCTTTATCTTGCCGTTGTT
CTCGACCTGTGGTCGCGTGCAGTTATAGGCTGGTCGATGTCATCGCGAATGACGGCGGCGCTGGCATGTAATGCACTGCAAATGGCACTGTGGAGGCGAA
AGCGTCCACAAAACGTTATTGTACATACCGATCGCGGTGGTCAGTATTGTTCAGCGGATTATCAAGCGATGCTGAATGAGCATAATCTGCGTGGCAGTAT
GAGTGCGAAGGGATGTTGTTACGACAATGCCTGCGCGGAAAGCTTCTTTCACTCGCTAAAAGTAGAATGTATCCACGGCGAACGCTTTGTCAGCAGGGAA
ATAATGCGAACAGCGGTGTTTAATTATATCGAGTGTGATTACAATCGCTGGCGTCGTCACAGTGCCTGTGGCGGACTCAGCCCGGAACAATTTGAAAACC
AGAACCTCGCTTAGGGCCGTGTCCACATTAGGTGGGTAGGATCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 67 | 363 | + | No |
Description : First part of the transposase
ORF sequence :
MTKSVSTKKPRKQHTPEFRDEALKLAGRIGVAAAARELSLYESQLYAWRSKQQQQMTSSERESELAVENARLKRQLAERDEELAILQKAATYFAKRLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 309 | 1214 | + | No |
Description : Second part of the transposase
ORF sequence :
GVGHSPKGRDILREAPEMKYVFIEKHRAEFTVRTMCRMLRVARSGWYAWRVRRHQISPRQQFRLTCDGAVRKAFGDAKQRYGAPRLADELPAYNIKTIAA
SLRRQGLRAKAARKFSPVSYREHGLPVSENLLKQDFYASGPNQKWAGDFTYLRTDEGWLYLAVVLDLWSRAVIGWSMSSRMTAALACNALQMALWRRKRP
QNVIVHTDRGGQYCSADYQAMLNEHNLRGSMSAKGCCYDNACAESFFHSLKVECIHGERFVSREIMRTAVFNYIECDYNRWRRHSACGGLSPEQFENQNL
A
SLRRQGLRAKAARKFSPVSYREHGLPVSENLLKQDFYASGPNQKWAGDFTYLRTDEGWLYLAVVLDLWSRAVIGWSMSSRMTAALACNALQMALWRRKRP
QNVIVHTDRGGQYCSADYQAMLNEHNLRGSMSAKGCCYDNACAESFFHSLKVECIHGERFVSREIMRTAVFNYIECDYNRWRRHSACGGLSPEQFENQNL
A
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 67 | 1214 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKSVSTKKPRKQHTPEFRDEALKLAGRIGVAAAARELSLYESQLYAWRSKQQQQMTSSERESELAVENARLKRQLAERDEELAILQKGRDILREAPEMK
YVFIEKHRAEFTVRTMCRMLRVARSGWYAWRVRRHQISPRQQFRLTCDGAVRKAFGDAKQRYGAPRLADELPAYNIKTIAASLRRQGLRAKAARKFSPVS
YREHGLPVSENLLKQDFYASGPNQKWAGDFTYLRTDEGWLYLAVVLDLWSRAVIGWSMSSRMTAALACNALQMALWRRKRPQNVIVHTDRGGQYCSADYQ
AMLNEHNLRGSMSAKGCCYDNACAESFFHSLKVECIHGERFVSREIMRTAVFNYIECDYNRWRRHSACGGLSPEQFENQNLA
YVFIEKHRAEFTVRTMCRMLRVARSGWYAWRVRRHQISPRQQFRLTCDGAVRKAFGDAKQRYGAPRLADELPAYNIKTIAASLRRQGLRAKAARKFSPVS
YREHGLPVSENLLKQDFYASGPNQKWAGDFTYLRTDEGWLYLAVVLDLWSRAVIGWSMSSRMTAALACNALQMALWRRKRPQNVIVHTDRGGQYCSADYQ
AMLNEHNLRGSMSAKGCCYDNACAESFFHSLKVECIHGERFVSREIMRTAVFNYIECDYNRWRRHSACGGLSPEQFENQNLA
Blast result :
Comments
ISPeat2 is 92% aa similar to ISEal1.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Nikolaichik Y. (2016) Direct submission to ISfinder