ISPa64

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JIEU01000003 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa BWH056
DNA section
IS Length : 2540 bp

Ends


IR Length : 20/26

IRL : tgttgatttccatccagaagtgacccactaagcccggcgatttccatcca
IRR : tgttgagttccatgcaatcctgacccggcatttccatccaatattgaccc

Insertion site


Left flankDirect repeatRight flankDR Length
CTCCATTATTCaatctggaGAAATTCCATGA9

DNA sequence

TGTTGATTTCCATCCAGAAGTGACCCACTAAGCCCGGCGATTTCCATCCAAATCTGACCCACGTAAGAACCCTAACCTGCTGTTTTTAGCGGCAGGAGCA
CAGGAGTGATTGACGTGGCGACACTGAGTGTCATCAGACGTTGGGCATTGCGTGAGCAACTGTCCATCCGGGAGATAGCCCGACGCACGGGCCTGTCTCG
CAATACCATCCGCAAGTACCTTCGCGAGGGCGAGGCGGCGCCCAGCTACGCGAAGCGGGTCAGCCCGAGCAAGCTCGACGCGTATGCCGAGAAGCTCGGC
GCCTGGCTCAAGAGCGAGGCATCGAGGTCACGCAAGCAGCGGCGCACCCTCAAGCAGATGCATGCCGACCTGGTCGCGCTCGGCTACCAGGGCTCCTACA
ACCGCGTGGCGGCCTTCGCCCGAGCGTGGCACGCACATCGCCACGAGGTCGAGCAGACCACCGGCCGAGGCACCTTCGTGCCGCTGGCCTTCGGTCCAGG
CGAGGCGTTCCAGTTCGACTGGAGCGAGGACTGGGCGGTCATCGGCGGGGAGCGCACCAAGCTCCAGGTCGCCCACTTCAAGCTCAGCCACAGCCGGGCC
TTCCTGGTTCGGGCGTATCCGCTGCAGACCCACGAGATGCTGTTCGACGCGCACAACCGCGCCTTCGAGGTGTTGGGCGGCGTGCCGCGACGAGGCATCT
ACGACAACATGAAGACGGCCGTGGATCGGGTGCGCCGAGGCAAGGAGCGCGACGTCAACGCGCGCTTCAGCGCCATGGTCAGCCACTACCTGTTCGAGGC
CGAGTTCTGCAACCCGGCTTCGGGCTGGGAGAAGGGTCAGGTGGAGAAGAACGTGCGCGATGCGCGTCACCGGCTGTGGCAGCCCGTCCCGGCGTTCACG
ACGTTGCCGCTGCTCAACGACTGGCTCGAGCACCGCTGCAAGGCGCTGTGGAGCGAAATCGCCCACGGCAAGCTGCCCGGCACCCTGGCCGACGTGTGGG
CCCAGGAGCGGCCGCTGCTGATGCCAATGCCTCGTCCCTTCGACGGCTTCGTCGAGCACACCAAGCGCGTCTCGCCTACCTGCCTGGTGCACTTCGAGCG
CAACCGCTACAGCGTGCCCGCGTCCTATGCGAACCGCCCGGTGAGCCTGCGGGTCTACGCAGACCGCCTGGTCGTGGCGGCCGAGGGGCAGCTCCTGTGC
GAGCACACGCGGATCATCGACCGCCGTCACGACACGGCCGGCCAGACCGTCTACGACTGGCGTCACTACTTGGCGGTGCTGCAACGCAAGCCCGGGGCGC
TCAGGAACGGCGCGCCCTTCAGTGAACTGCCGCAGGGCTTCCGACGGTTGCAGACCGTCCTGCTCCAGCAGCCCGGCGGGGACCGGGAGATGGTAGAGAT
CCTCGCGCTCGTCTTGCATCACGACGAGCAGGCCGTGCTGACCGCGGTCGAGCTGGCCCTGGAAGCTGGGGTGGCGAGCAAGACGCATGTGCTCAACGTC
CTGCACCGCCTGCTCGATGGCAAGCCAGTAGCGCCTGCGCCTGTGACGGCACCTCAGGCGCTGCGACTGGCGAACGAGCCCCAGGCCAACGTGTTGCGCT
ATGACTCCCTCAGAGGACGCGTGACTGCAGTCGATCCGATCATCATCCAGGAGGTGCGCCATGCGCCATGACCCCGCCAGTGCAGCCATCGTCATCATGC
TGCGTGAACTCAAGATGCACGGCATGGCGCACGCCGTGGCCGAACTCGCCGAGCAGGGCTCGCCGGCCTTCGAGGCTGCACAGCCGATGCTCTCGCAGTT
GCTCAAGGCCGAGACGGCCGAACGCGAGGTGCGCTCGGTGGCCTACCAGTTGAAGCTGGCACGCTTCCCGGCCTACCGCGACCTCACAGGCTTCGACTTC
CAGCACAGCGAGGTCAATGAAGCCTTGGTGCGGCAGTTGCATCGATGCGAGTTCATGGAGGACGCCCAGAACGTGGTGCTCGTCGGTGGCCCGGGCACGG
GCAAGACCCACATCGCCACGGCGCTGGGTGTGCAGGCCATCGAGCATCACCACCGGCGTGTGAGGTTCTTCTCCACGGTCGAACTGGTCAACGCCCTAGA
GCAGGAGAAGGCCAGCGGCAACCCGGGGCACATCGCGCATCGGCTGATGTATGCGGACATGGTGATCCTCGACGAGCTCGGCTACCTGCCGTTCAGCGCC
TCGGGCGGGGCGCTGTTGTTCCATCTGCTCTCGAAGCTCTACGAGCGCACGAGCGTGGTGATCACGACGAACCTGAGCTTCAGCGAGTGGGCCAGCGTGT
TCGGCGATGCGAAGATGACGACGGCGCTGCTGGACCGGCTCACGCATCATTGCCACATCCTGGAGACCGGCAACGACAGCTTCCGGTTCAAGCACAGCTC
GGCTCAACCCGTTCAGGCAGACCAACCCAGAAAGAAGGAGAAGACCAAGAACTTATCCACACCGTGAGCCGCCAGGCTCGCAAAGAGGGTGGGTCAATAT
TGGATGGAAATGCCGGGTCAGGATTGCATGGAACTCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1566 bp518 aa1061671+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLREGEAAPSYAKRVSPSKLDAYAEKLGAWLKSEASRSRKQRRTLKQMHADLVALGYQGSYNR
VAAFARAWHAHRHEVEQTTGRGTFVPLAFGPGEAFQFDWSEDWAVIGGERTKLQVAHFKLSHSRAFLVRAYPLQTHEMLFDAHNRAFEVLGGVPRRGIYD
NMKTAVDRVRRGKERDVNARFSAMVSHYLFEAEFCNPASGWEKGQVEKNVRDARHRLWQPVPAFTTLPLLNDWLEHRCKALWSEIAHGKLPGTLADVWAQ
ERPLLMPMPRPFDGFVEHTKRVSPTCLVHFERNRYSVPASYANRPVSLRVYADRLVVAAEGQLLCEHTRIIDRRHDTAGQTVYDWRHYLAVLQRKPGALR
NGAPFSELPQGFRRLQTVLLQQPGGDREMVEILALVLHHDEQAVLTAVELALEAGVASKTHVLNVLHRLLDGKPVAPAPVTAPQALRLANEPQANVLRYD
SLRGRVTAVDPIIIQEVRHAP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
837 bp268 aa16612497+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPASAAIVIMLRELKMHGMAHAVAELAEQGSPAFEAAQPMLSQLLKAETAEREVRSVAYQLKLARFPAYRDLTGFDFQHSEVNEALVRQLHRCEFME
DAQNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEQEKASGNPGHIAHRLMYADMVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSFRFKHSSAQPVQADQPRKKEKTKNLSTP

 

Blast result :
Comments
ISPa64 is 90% (transposase and 92% (IS21 helper) aa similar to IS1600.
References