ISPpn1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP015372 ND Pandoraea pnomenusa
Pandoraea pnomenusa MCB032 plasmid 1
DNA section
IS Length : 2540 bp

Ends


IR Length : 19/26

IRL : TGTTGATTTCCATCTAGAAGTGACCCACTAAGCCCGGTGATTTCCATCTA
IRR : TGTTGAGTTCCATGCAATCCTGACCCGGCATTTCCATCCAATATTGACCC

Insertion site


Left flankDirect repeatRight flankDR Length
GGACAAGAGCGTCATCGTCTTCAACAGC8

DNA sequence

TGTTGATTTCCATCTAGAAGTGACCCACTAAGCCCGGTGATTTCCATCTAAATCTGACCCACGTAAGAACCCTAACCTGCTGCTTTTAGCGGCAGGAGCA
CAGGAGTGATTGACGTGGCGACACTGAGTGTCATCAGACGTTGGGCATTGCGTGAGCAACTGTCCATCCGGGAGATAGCCCGACGCACGGGCCTGTCTCG
CAACACCATCCGCAAGTACCTTCGCGAGGGTGAGGCGGCGCCCAGCTACGCGAAGCGGGTCAGCCCGAGCAAGCTCGATGCGTATGCAGAGAAGCTTGGC
GCCTGGCTCAAGAGCGAGGCATCGAGATCGCGCAAGCAGCGGCGCACCCTCAAACAGATGCATGCAGACTTGGCCGCGCTCGGCTACCAGGGCTCCTACA
ACCGCGTGGCCGCCTTCGCGCGGGCATGGCACGCGCATCGCCACGAGGTGGAGCAGACCACCGGTCGAGGCACATTCGTGCCGCTGGCCTTCGGCCCGGG
CGAGGCGTTCCAGTTCGACTGGAGCGAGGACTGGGCCGTCATCGGCGGGGAGCGCACCAAGCTGCAGGTCGCGCACTTCAAGCTCAGCCACAGCCGGGCC
TTCCTGGTGCGGGCATACCCACTGCAGACCCACGAGATGCTGTTCGACGCGCACAACCGCGCCTTCGAAGTGTTGGGCGGCGTGCCGCGACGAGGCATCT
ACGACAACATGAAGACGGCCGTGGATCGCGTGCGCCGCGGCAAGGAGCGCGACGTCAACGCGCGCTTCAGCGCCATGGTCAGCCACTACCTGTTCGAGGC
CGAGTTCTGCAACCCGGCTTCGGGCTGGGAGAAGGGTCAGGTGGAGAAGAATGTGCGCGATGCGCGTCACCGGCTGTGGCAGCCCGTCCCGGCGTTCGCG
ACGCTGCCGATGCTCAACGACTGGCTCGAGCAACGCTGCAAGGCGCTGTGGGGCGAGATCACTCACGGCAAGCTGCCCGGCACTCTGGCCGACGTGTGGG
CCCAGGAGCGGCCGCTGCTGATGCCCGTGCCTCGTCCCTTCGACGGCTTCGTCGAGCACACCAAGCGCGTCTCACCCACCTGCCTGGTGCACTTCGAGCG
CAACCGCTACAGCGTGCCCGCGTCCTATGCGAACCGCCCGGTGAGCCTGCGGGTCTACGCAGACCGCCTGGTCGTGGCGGCCGAGGGGCAGCTCCTGTGC
GAGCACACGCGGATCATCGACCGTCGCCACGATACGGCCGGCCAGACCGTCTACGACTGGCGTCACTACCTGGCGGTGCTGCAACGCAAGCCGGGGGCGT
TGAGGAACGGCGCGCCCTTCAGTGAGCTGCCGCAGGGCTTCAGACGGTTGCAGACCGTCCTGCTCAAGCAGCCCGGTGGCGACCGGGAGATGGTGGAGAT
CCTCGCGCTCGTCTTGCACCACGACGAGCAGGCCGTGCTGACCGCGGTCGAGCTGTCACTGGAAGCGGGCGTCGCGAGCAAGACGCATGTGCTGAACGTC
CTGCATCGGCTGCTCGACGGCAAGCCGGTGGCGCCAGCGCCGGTGACGGCGCCTCAGGCGTTGCGCCTTGCAAACGAACCCCAGGCCAATGTGCTGCGCT
ATGACTCCCTCAGAGGACGCGTGACTGCAGCCGATCCGATCATCATCCAGGAGGTGCGCCATGCGCCATGACCCCGCCAGTGCAGCCATCGTCATCATGC
TGCGTGAACTCAAGATGCACGGCATGGCGCACGCCGTGGCCGAACTCGCCGAGCAGGGCTCGCCGGCGTTCGAGGCCGCGCAGCCGATGCTCTCGCAGTT
GCTCAAGGCCGAGACGGCCGAACGCGAGGTGCGCTCGGTGGCCTACCAGTTGAAGCTGGCACGCTTCCCGGCCTACCGCGACCTCACAGGCTTCGACTTC
CAGCACAGCGAGGTCAATGAAGCCTTGGTGCGGCAGTTGCATCGATGCGAGTTCATGGAGGACGCCCAGAACGTGGTGCTCGTCGGTGGCCCGGGCACGG
GCAAGACCCACATCGCCACGGCGCTGGGTGTGCAGGCCATCGAGCATCACCACCGGCGTGTGAGGTTCTTCTCCACGGTCGAACTGGTCAACGCGCTCGA
GCAGGAGAAGGCCAGCGGCAACCCGGGCCACATCGCGCATCGGCTGATGTACGCGGACATGGTGATCCTCGACGAGCTCGGCTACCTGCCGTTCAGCGCC
TCGGGCGGGGCGTTGTTGTTCCATCTGCTCTCGAAGCTCTACGAGCGCACGAGCGTGGTGATCACGACGAACCTGAGCTTCAGCGAGTGGGCCAGCGTGT
TCGGCGATGCGAAGATGACGACGGCGCTGCTGGACCGGCTCACGCATCACTGCCATATCCTGGAGACCGGCAACGACAGCTTCCGCTTCAAGCACAGCTC
GGCTCAACCCGTTCAGGCAGACCAACCCAGAAAGAAGGAGAAGACCAAGAACTTATCCACACCGTGAGCCACCAGGCTCGCAAAGAGGGTGGGTCAATAT
TGGATGGAAATGCCGGGTCAGGATTGCATGGAACTCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1566 bp521 aa1061671+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDVATLSVIRRWALREQLSIREIARRTGLSRNTIRKYLREGEAAPSYAKRVSPSKLDAYAEKLGAWLKSEASRSRKQRRTLKQMHADLAALGYQGSYNR
VAAFARAWHAHRHEVEQTTGRGTFVPLAFGPGEAFQFDWSEDWAVIGGERTKLQVAHFKLSHSRAFLVRAYPLQTHEMLFDAHNRAFEVLGGVPRRGIYD
NMKTAVDRVRRGKERDVNARFSAMVSHYLFEAEFCNPASGWEKGQVEKNVRDARHRLWQPVPAFATLPMLNDWLEQRCKALWGEITHGKLPGTLADVWAQ
ERPLLMPVPRPFDGFVEHTKRVSPTCLVHFERNRYSVPASYANRPVSLRVYADRLVVAAEGQLLCEHTRIIDRRHDTAGQTVYDWRHYLAVLQRKPGALR
NGAPFSELPQGFRRLQTVLLKQPGGDREMVEILALVLHHDEQAVLTAVELSLEAGVASKTHVLNVLHRLLDGKPVAPAPVTAPQALRLANEPQANVLRYD
SLRGRVTAADPIIIQEVRHAP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
807 bp268 aa16612467+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPASAAIVIMLRELKMHGMAHAVAELAEQGSPAFEAAQPMLSQLLKAETAEREVRSVAYQLKLARFPAYRDLTGFDFQHSEVNEALVRQLHRCEFME
DAQNVVLVGGPGTGKTHIATALGVQAIEHHHRRVRFFSTVELVNALEQEKASGNPGHIAHRLMYADMVILDELGYLPFSASGGALLFHLLSKLYERTSVV
ITTNLSFSEWASVFGDAKMTTALLDRLTHHCHILETGNDSFRFKHSSAQPVQADQPRKKEKTKNLSTP

 

Blast result :
Comments
ISPpn1 is 90% (transposase) and 92% (IS21 helper) aa similar to IS1600.
References
1] ISfinder annotation (2016)
2] Chao,H.-J. and Zhou,N.-Y. (2016) Direct GenBank submission.