ISPa71
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LC075717 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa NCGM 2991 |
DNA section
IS Length : 1426 bp
Ends
IR Length : 12
IRL : tgatgggaTGGACTcctcccgcgtcaccaaacggcttcgaagagccagaC
IRR : ----tgtaTGGACTCCTCCgtcaagcagtcgttgccgtagcggatcgttg
Comments : Not located at the termini of the IS.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGGCTTGAA | TGAATTGTTA | 0 |
DNA sequence
TGATGGGATGGACTCCTCCCGCGTCACCAAACGGCTTCGAAGAGCCAGACTGATGTTTCCATCAGGACACGGGATCACGAGAGGAGTCCACGCCATGCAT
GCTAGTACCGTCGCCATCGATTTGGCAAAAGAAGTCTTCGAGCTGGCCTTCGCCGATGCGCAGGGCCGGATCATCGAGCGCAAGCGCCTCTCGCGCAGGG
CGTTCATCCGGTCGCTGGAGACCCGGCCGCCGTTGCGGGTGCTGATGGAGGCCTGCGGGGGTGCGCACTGCTGGGCGCGCCGCTTCCAGCGCCAGGGCCA
CGCGGTGCGCCTGCTTCCTGCGCGGGACGTGCGCCCCTACGTGCGCGGCAACAAGACCGACCGCAACGACGTGGCCGGGATACTGGAAGCTGATCGCTGC
GCCGGCATTGCGCCGGTGCCGGTCAAGACCCCGGAGCAGCAGGGCATCCAGGCGCTGCATCGGTTGCGCGAACACCTCAAGGCCGAACGCACGGCCACGC
TCAACCTGCTGCGGGGCGTGCTGCGCGAGTTCGGCGTGGCCGTGCCGCTGGGGTCGACCAAGGTGCCCGCCGCCGTGCGCGATGCGCTGGAAGACGGGGA
CAACGACCTGCCGATGGCGCTGCGCCACAGCCTGGCCGAGCAGATCGACAGGCTGTCGCAGCTGGCCAGGGACATGCTGGCAATCGAACAGCGGCTGGAG
GAATTCGCCTCGCGGGACGTGGCAGTGCAGCGTTACCGCACGGTCCCGGGCGTGGGTCTGCTGACGGCGACCGCGCTACGGGCCAGTGCCGGCGATCTGT
CGCGCTTCCGTAGCGGACGCCAGTTCTCGGCGTGGCTGGGCCTGACCCCGCGCGAGCATTCCTCGGGTCAGCAGCGCCGGCTGGGAGGCCTGACCAAGCG
CGGCGATGTCTACGTAAGAACGCTGCTGATCCACGGCGCCCGCGCGGTACTGCAGGCGGCGCGGATGAAGCAGCGTCGTGGACAAGACCTCGATCCGCTG
CAGGCCTGGGCACTGAAGGTCCAGGACACCCGCGGCCACAACAAGGCCGCCTGCGCCCTGGCCAACAAGCTGGCCCGGCGGCTGTGGGCGATGGACCACC
ACGGCACGCCGTTCGATCCGCGCCACGTCAGCGAACGCCCCCGCGCTGCGTAGCGCGACGGCTTCGACACACGTTCACCGCTTCAACGTCGAGAGTTGCC
TGACACGATCTTGATCATGGCGAAGGGTTCGGTCCCGGAGCGGACAACGCCGATAACAATGCTGGCCTGGCAGGCCGCTTGAACGATAGGCACCCGCCCC
GCGGATTCCATGATGGCCCGGGCATCAACGCCCACCCCAAGGCCGGATACACGACTGCAACCGCACCGCTTCCCGACGATCAACGATCCGCTACGGCAAC
GACTGCTTGACGGAGGAGTCCATACA
GCTAGTACCGTCGCCATCGATTTGGCAAAAGAAGTCTTCGAGCTGGCCTTCGCCGATGCGCAGGGCCGGATCATCGAGCGCAAGCGCCTCTCGCGCAGGG
CGTTCATCCGGTCGCTGGAGACCCGGCCGCCGTTGCGGGTGCTGATGGAGGCCTGCGGGGGTGCGCACTGCTGGGCGCGCCGCTTCCAGCGCCAGGGCCA
CGCGGTGCGCCTGCTTCCTGCGCGGGACGTGCGCCCCTACGTGCGCGGCAACAAGACCGACCGCAACGACGTGGCCGGGATACTGGAAGCTGATCGCTGC
GCCGGCATTGCGCCGGTGCCGGTCAAGACCCCGGAGCAGCAGGGCATCCAGGCGCTGCATCGGTTGCGCGAACACCTCAAGGCCGAACGCACGGCCACGC
TCAACCTGCTGCGGGGCGTGCTGCGCGAGTTCGGCGTGGCCGTGCCGCTGGGGTCGACCAAGGTGCCCGCCGCCGTGCGCGATGCGCTGGAAGACGGGGA
CAACGACCTGCCGATGGCGCTGCGCCACAGCCTGGCCGAGCAGATCGACAGGCTGTCGCAGCTGGCCAGGGACATGCTGGCAATCGAACAGCGGCTGGAG
GAATTCGCCTCGCGGGACGTGGCAGTGCAGCGTTACCGCACGGTCCCGGGCGTGGGTCTGCTGACGGCGACCGCGCTACGGGCCAGTGCCGGCGATCTGT
CGCGCTTCCGTAGCGGACGCCAGTTCTCGGCGTGGCTGGGCCTGACCCCGCGCGAGCATTCCTCGGGTCAGCAGCGCCGGCTGGGAGGCCTGACCAAGCG
CGGCGATGTCTACGTAAGAACGCTGCTGATCCACGGCGCCCGCGCGGTACTGCAGGCGGCGCGGATGAAGCAGCGTCGTGGACAAGACCTCGATCCGCTG
CAGGCCTGGGCACTGAAGGTCCAGGACACCCGCGGCCACAACAAGGCCGCCTGCGCCCTGGCCAACAAGCTGGCCCGGCGGCTGTGGGCGATGGACCACC
ACGGCACGCCGTTCGATCCGCGCCACGTCAGCGAACGCCCCCGCGCTGCGTAGCGCGACGGCTTCGACACACGTTCACCGCTTCAACGTCGAGAGTTGCC
TGACACGATCTTGATCATGGCGAAGGGTTCGGTCCCGGAGCGGACAACGCCGATAACAATGCTGGCCTGGCAGGCCGCTTGAACGATAGGCACCCGCCCC
GCGGATTCCATGATGGCCCGGGCATCAACGCCCACCCCAAGGCCGGATACACGACTGCAACCGCACCGCTTCCCGACGATCAACGATCCGCTACGGCAAC
GACTGCTTGACGGAGGAGTCCATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1059 bp | 352 aa | 95 | 1153 | + | No |
Chemistry : DEDD
ORF sequence :
MHASTVAIDLAKEVFELAFADAQGRIIERKRLSRRAFIRSLETRPPLRVLMEACGGAHCWARRFQRQGHAVRLLPARDVRPYVRGNKTDRNDVAGILEAD
RCAGIAPVPVKTPEQQGIQALHRLREHLKAERTATLNLLRGVLREFGVAVPLGSTKVPAAVRDALEDGDNDLPMALRHSLAEQIDRLSQLARDMLAIEQR
LEEFASRDVAVQRYRTVPGVGLLTATALRASAGDLSRFRSGRQFSAWLGLTPREHSSGQQRRLGGLTKRGDVYVRTLLIHGARAVLQAARMKQRRGQDLD
PLQAWALKVQDTRGHNKAACALANKLARRLWAMDHHGTPFDPRHVSERPRAA
RCAGIAPVPVKTPEQQGIQALHRLREHLKAERTATLNLLRGVLREFGVAVPLGSTKVPAAVRDALEDGDNDLPMALRHSLAEQIDRLSQLARDMLAIEQR
LEEFASRDVAVQRYRTVPGVGLLTATALRASAGDLSRFRSGRQFSAWLGLTPREHSSGQQRRLGGLTKRGDVYVRTLLIHGARAVLQAARMKQRRGQDLD
PLQAWALKVQDTRGHNKAACALANKLARRLWAMDHHGTPFDPRHVSERPRAA
Blast result :
Comments
ISPa71 is 87% similar to ISPa25.
ISPa71 is embedded within the attC recombination site of an integron gene cassette. Its boundaries have been deduced from the boundaries of the non disrupted attC site. Its IR are not at the termini of the IS.
There is an empty site in Pseudomonas aeruginosa aacA37 (Accession number : NG_047320).
ISPa71 is embedded within the attC recombination site of an integron gene cassette. Its boundaries have been deduced from the boundaries of the non disrupted attC site. Its IR are not at the termini of the IS.
There is an empty site in Pseudomonas aeruginosa aacA37 (Accession number : NG_047320).
References