ISNma26
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001934 | ND | Natrialba magadii | Natrialba magadii ATCC 43099 pNMAG02 |
DNA section
IS Length : 1414 bp
Ends
Left end : GCAAGGCGGATTCCCCGCCCTTCCGAAAACCTGCGGTTTTCGGCTTTCGCAAACCTTTGATTTGCGTCAACACCGTGGGCGGGGTTGAAGCCGACACCAC II struct. : Yes
Right end : CGAGGACGCCGAGTTAGCCAGATACACTGGTTCCCCCAATACTGGGGCAGAGGCCGAGTTCACTGACAAGCCCCGCGCCACCGTGCGCGGGGCAGTTGAC II struct. : Yes
Comments : -
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CCAGTTGGCTTCAAACTA | GGTA | TGTGCCAAGCAGGCCGA | TGAC |
DNA sequence
GCAAGGCGGATTCCCCGCCCTTCCGAAAACCTGCGGTTTTCGGCTTTCGCAAACCTTTGATTTGCGTCAACACCGTGGGCGGGGTTGAAGCCGACACCAC
CCCGAATTTTATCAACCTATAACTCTTTATTGAACATATGGCGTCGGACGAGTATCACCGTCGAACACTCATTACTCGCCTCACTCTCCGTAGTGACGAG
GATGAGCAACTGCTCCTCAACACGATCGAGGAGTGGCTCGACGGTTGTAACATCGCTAGCACTCTCGCGTGGAACGACTGTCACACCAAGTCCGATGTCC
GACAACTCGCCAAACACAAAATCAAGCAGGAGACCAAGTTAGGCGACCAACACGCCACGCTCGCCTGCCACGAAGTCTCCGGTGCAATCAAGTCCTGCAT
CGAACGCCGCAAAAACGGGAAGAAAGCCAGCCAACCACAATTCACAACCCGGTCGATGACATTCGACCGACGTACCCTCACCGTCTTCCCCGACCAAGAA
AAGGTTACACTCACCACGCTCGGAGACCACAGCCGCGTCCACGCCGACCTATGCCTTCCCGAAAACGAAGACGGCTATCAATACGACTACCTCTATAGTG
ATGAGTGGGAGTACACAGAATCCACACTCCACTACCGAGATGGTGACTGGTACATCCATCTTGGCTATCGAACACCAACGGAAGACGGCGAACCCGAGGC
AGCGACCGAGAACGGAACGGTTCTCGGTGTTGACCTCGGCGTCAACCAAATCGCTGTGACGAGTACAGCCAGGTTCTTCGACGCGGGTGAACTCAACCAC
TACCGCCGTGAATGTGAGAACACACGTGGCAGTCTCCAGCAGACCGGGACGCAATCCGCCCACCGAACCATCGAATCCCTATCCGGCAGGGAAAACCGAT
ACGTCAAACACGTTTTGCACAGCGTGGCGAACGGTATCATAGAAGAAGCCCTTGAACACAACTGCGATGGTATCGTGTTCGAGGAACTGGACGGGATTCG
AGAGGACTTGCCAGAAGCGGCGTGGCATTCGGAGTGGGCGTTCCGCAAACTGAAAGATTTCGTGGAGTACAAAGCCGAGTACGAGGGCCTGTTCGTTGAT
ACGACAAATCCTCGAAATACGTCGAAACGGTGTAACGAGTGTGGATTCACGCACGACGATAACCGGTACGCTCGGCAGTTCGAGTGCCAGTCGTGTGGGA
AGACGAATCACGCAGATTACAACGCGGCGAAAAACATTGCAGAACTGTATCTCCGGCGGGGCCATCAGTCGTCCGGTCGGAGGAGCGTCAGTCAATACGC
TCTGAAGTCAGGAGCGAGGACGCCGAGTTAGCCAGATACACTGGTTCCCCCAATACTGGGGCAGAGGCCGAGTTCACTGACAAGCCCCGCGCCACCGTGC
GCGGGGCAGTTGAC
CCCGAATTTTATCAACCTATAACTCTTTATTGAACATATGGCGTCGGACGAGTATCACCGTCGAACACTCATTACTCGCCTCACTCTCCGTAGTGACGAG
GATGAGCAACTGCTCCTCAACACGATCGAGGAGTGGCTCGACGGTTGTAACATCGCTAGCACTCTCGCGTGGAACGACTGTCACACCAAGTCCGATGTCC
GACAACTCGCCAAACACAAAATCAAGCAGGAGACCAAGTTAGGCGACCAACACGCCACGCTCGCCTGCCACGAAGTCTCCGGTGCAATCAAGTCCTGCAT
CGAACGCCGCAAAAACGGGAAGAAAGCCAGCCAACCACAATTCACAACCCGGTCGATGACATTCGACCGACGTACCCTCACCGTCTTCCCCGACCAAGAA
AAGGTTACACTCACCACGCTCGGAGACCACAGCCGCGTCCACGCCGACCTATGCCTTCCCGAAAACGAAGACGGCTATCAATACGACTACCTCTATAGTG
ATGAGTGGGAGTACACAGAATCCACACTCCACTACCGAGATGGTGACTGGTACATCCATCTTGGCTATCGAACACCAACGGAAGACGGCGAACCCGAGGC
AGCGACCGAGAACGGAACGGTTCTCGGTGTTGACCTCGGCGTCAACCAAATCGCTGTGACGAGTACAGCCAGGTTCTTCGACGCGGGTGAACTCAACCAC
TACCGCCGTGAATGTGAGAACACACGTGGCAGTCTCCAGCAGACCGGGACGCAATCCGCCCACCGAACCATCGAATCCCTATCCGGCAGGGAAAACCGAT
ACGTCAAACACGTTTTGCACAGCGTGGCGAACGGTATCATAGAAGAAGCCCTTGAACACAACTGCGATGGTATCGTGTTCGAGGAACTGGACGGGATTCG
AGAGGACTTGCCAGAAGCGGCGTGGCATTCGGAGTGGGCGTTCCGCAAACTGAAAGATTTCGTGGAGTACAAAGCCGAGTACGAGGGCCTGTTCGTTGAT
ACGACAAATCCTCGAAATACGTCGAAACGGTGTAACGAGTGTGGATTCACGCACGACGATAACCGGTACGCTCGGCAGTTCGAGTGCCAGTCGTGTGGGA
AGACGAATCACGCAGATTACAACGCGGCGAAAAACATTGCAGAACTGTATCTCCGGCGGGGCCATCAGTCGTCCGGTCGGAGGAGCGTCAGTCAATACGC
TCTGAAGTCAGGAGCGAGGACGCCGAGTTAGCCAGATACACTGGTTCCCCCAATACTGGGGCAGAGGCCGAGTTCACTGACAAGCCCCGCGCCACCGTGC
GCGGGGCAGTTGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1194 bp | 397 aa | 138 | 1331 | + | No |
AG : TnpB
ORF sequence :
MASDEYHRRTLITRLTLRSDEDEQLLLNTIEEWLDGCNIASTLAWNDCHTKSDVRQLAKHKIKQETKLGDQHATLACHEVSGAIKSCIERRKNGKKASQP
QFTTRSMTFDRRTLTVFPDQEKVTLTTLGDHSRVHADLCLPENEDGYQYDYLYSDEWEYTESTLHYRDGDWYIHLGYRTPTEDGEPEAATENGTVLGVDL
GVNQIAVTSTARFFDAGELNHYRRECENTRGSLQQTGTQSAHRTIESLSGRENRYVKHVLHSVANGIIEEALEHNCDGIVFEELDGIREDLPEAAWHSEW
AFRKLKDFVEYKAEYEGLFVDTTNPRNTSKRCNECGFTHDDNRYARQFECQSCGKTNHADYNAAKNIAELYLRRGHQSSGRRSVSQYALKSGARTPS
QFTTRSMTFDRRTLTVFPDQEKVTLTTLGDHSRVHADLCLPENEDGYQYDYLYSDEWEYTESTLHYRDGDWYIHLGYRTPTEDGEPEAATENGTVLGVDL
GVNQIAVTSTARFFDAGELNHYRRECENTRGSLQQTGTQSAHRTIESLSGRENRYVKHVLHSVANGIIEEALEHNCDGIVFEELDGIREDLPEAAWHSEW
AFRKLKDFVEYKAEYEGLFVDTTNPRNTSKRCNECGFTHDDNRYARQFECQSCGKTNHADYNAAKNIAELYLRRGHQSSGRRSVSQYALKSGARTPS
Blast result :
Comments
ISNma26 is 82% (TnpB) aa similar to ISHwa27.
In the genome, this element is targetted by a MITE : MITENma1, bases 128-135 (TATTGAAC) are the duplicated target of this element. ISNma26 sequence was reconstructed in silico by deletion of MITENma1 and of one target repetition.
In the genome, this element is targetted by a MITE : MITENma1, bases 128-135 (TATTGAAC) are the duplicated target of this element. ISNma26 sequence was reconstructed in silico by deletion of MITENma1 and of one target repetition.
References
1] Friedhelm Pfeiffer (2016) Direct submission.
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165.
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. BMC Genomics (2012) 13: 165.