ISfinder Mirror Site

ISHhi1

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP002921	ND	Haloarcula hispanica	Haloarcula hispanica N601

DNA section

IS Length : 1066 bp

Ends

IR Length : 13/23

IRL : CTGTCGGCTGAAAATTCTTTAGGCTAGAGTGTGACTGTCGAGTATGGCCG
IRR : CTCACGGACATAAATACGTGAAGACATCTGGAACTTCAATTCGATCGAGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGGGTTATTGA	TA	TTCATCGAATA	2
GCAGAGAGTTA	TA	GAAACAGCGT	2

DNA sequence

CTGTCGGCTGAAAATTCTTTAGGCTAGAGTGTGACTGTCGAGTATGGCCGCGCTTGACGACGCCACTCTCGACGACCTCCGCGAGGCTCTCGCGGAGGTC
GAGGACAAGAAGCCGACACAGCGGCTAATGGCGGTGATTAACTACCTCGAAGAAGACAGCGCAACGATGGCAGAAGTAGCTGAGCGGTACGGATACACCG
GTCCATGGCTGTCTCGATGGGTTGGGCGGCTCGACCGGCTCGCTGATGAGCCGGTCGAGCAAGTCGTCTATGACGATCCACGTGAAGGCAGAACAGCAGA
ACTCTCTGGTGAACAATACGACCAGTTCGTTGAGGCTCTCCACGAATCACCCGAAGAAGTTGGACTAGACGCGCCCGCGTGGTCTGTTCCACTAGCCCGT
CACTATCTAGCCGAAGAATTCGACGTTGAGTATTGTGAACGCCACGTCCGTCGATTGATGTCTGAGGCCGGGCTGTCCTGGAAGACAGCCCGGCCGGAGT
ACTACAAATCCGACGAGAGAGCTCAAGAAGCCTGGCAAGAAGGCTTCAAAAAAAGCGCGACAACCTGGACGACGAATACACGGTCCTGACCATCGATCAG
ACGCGTCAAGTGCTCTCGACGCTGATCTATGCGTGGTTTCCCGAAGGAGAGCGCCCGTCACTCCCGGTGACGGGCGCGTGGGACAGCATCAAACTACTCG
GTGCAATCAGCGATTCCGGCGAGACGTTCTTTCTACCCTGTGAGGAGAACTTCAACAGCGACACGACGAATCGCTTGCTAGACGCTCTCCAAACCGAATT
CGGCGAGAAAATCTGCGTCATCTTGGACAACGCCTCGTATTTCACGGCCAACGCAGTACAGGAATTCGTCGAAGACACGCCGATCGAACTGTGTTATCTT
CCGCGGGGTTCACCAGAGCTGAACCCCGCGGAAGAGTGCTGGCGACAACTCGATCAAGAGCTTGGCAACCGTCTGTTCGACACACTTGACGATCTGCGTG
ATGCTGCGCTCACTGCACTCGATCGAATTGAAGTTCCAGATGTCTTCACGTATTTATGTCCGTGAG

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
646 bp	181 aa	47	692	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAALDDATLDDLREALAEVEDKKPTQRLMAVINYLEEDSATMAEVAERYGYTGPWLSRWVGRLDRLADEPVEQVVYDDPREGRTAELSGEQYDQFVEALH
ESPEEVGLDAPAWSVPLARHYLAEEFDVEYCERHVRRLMSEAGLSWKTARPEYYKSDERAQEAWQEGFKKSATTWTTNTRS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
600 bp	199 aa	569	1168	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GRAVLEDSPAGVLQIRRESSRSLARRLQKKRDNLDDEYTVLTIDQTRQVLSTLIYAWFPEGERPSLPVTGAWDSIKLLGAISDSGETFFLPCEENFNSDT
TNRLLDALQTEFGEKICVILDNASYFTANAVQEFVEDTPIELCYLPRGSPELNPAEECWRQLDQELGNRLFDTLDDLRDAALTALDRIEVPDVFTYLCP

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1022 bp	339 aa	147	1168	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAALDDATLDDLREALAEVEDKKPTQRLMAVINYLEEDSATMAEVAERYGYTGPWLSRWVGRLDRLADEPVEQVVYDDPREGRTAELSGEQYDQFVEALH
ESPEEVGLDAPAWSVPLARHYLAEEFDVEYCERHVRRLMSEAGLSWKTARPEYYKSDERAQEAWQEGFKKRDNLDDEYTVLTIDQTRQVLSTLIYAWFPE
GERPSLPVTGAWDSIKLLGAISDSGETFFLPCEENFNSDTTNRLLDALQTEFGEKICVILDNASYFTANAVQEFVEDTPIELCYLPRGSPELNPAEECWR
QLDQELGNRLFDTLDDLRDAALTALDRIEVPDVFTYLCP

Blast result :

Comments

ISHhi1 is 89% aa similar to ISNamo15. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Friedhelm Pfeiffer (2016) Direct submission.
2] Liu,H., Wu,Z., Li,M., Zhang,F., Zheng,H., Han,J., Liu,J., Zhou,J., Wang,S., Xiang,H. (2011) J. Bacteriol. 193(21), 6086-6087