ISHahy2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002304 | ND | Halanaerobium hydrogeniformans | Halanaerobium hydrogeniformans |
DNA section
IS Length : 1212 bp
Ends
IR Length : 29/41
IRL : TGGATCGGGTATAATTAATTAAACAGTTTTTTCTCGGACATGATATAATA
IRR : TGGATTGTCAATACTTTTTACAACAATTTTTTATCGAACATTAAATATCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAATTGCTCATTTAAATTCCCCGGGAGAATTTAATAAAACTTAAAAAA | AGG | AGGACTTATTAATGTTAAAAGACATGGATAAAAAAGAAATTGAAAAGAAA | 3 |
| CCTACTAAAGAAATATTAATTATCATTTATAGTAGGGTGACCGTTAGGTT | TTTG | ATTTTAATAATTATTCTTCACCATAATAGTAATAGTTTTCATAAGTATAA | 4 |
| TGAAGCTAGCTATGAGTAGAATTAAATCAGGAGTGGTTAAATGAATTGGG | TATATTAATAAATATAATTTAGAATATTTAAACATTGATAATAATACTAA | 0 | |
| AATGCATTTCAGTATAATATTTTTACTTTTCAGTTAAATATTCTTTGTTA | ATC | CTTATAAGTGTATAATTGTATATGTAATAAAAACTTATGAGGAGGAATGC | 3 |
| TTTTTCATGATGGGTTAGTATTTCCTCTGCTACAATAGAGAATTCTTCAG | GAA | AGTGCAATTTTATAACCCTGTTGAGGATGAGATTTTAAAAGGTCATACTC | 3 |
| TAATATTCTATGATTGTCAAGCCTGCCTCAGATATTTATATCACAGTGAT | CCTCCAACATATTTTGAGACTCTCTGATCATTGTAGTATAACTCAACAGT | 0 | |
| CAGGTCTATATTATAATAGACATCAAAACCTTCCTCCTGGCTTAAGGCGT | GCA | TACATTACAGCATCGATCCACCTTATGCTTTCAACAGGTCTATTAGAACT | 3 |
| TCTTTCCATAAAAAAACACCTCCAAGTAGACAGTCTATTTTAATTAATTA | CTG | TCTACTTTGGAAGTATCATATCAAAAAAGTGGAGTTATCATTATAATATG | 3 |
DNA sequence
TGGATCGGGTATAATTAATTAAACAGTTTTTTCTCGGACATGATATAATAAGATAATAACAAACTAAAATGGAGGAAAATATCATGCCTACAAAATATCC
TGAAGAAATCAAAAGAAAAGTTGTTGCTCTGGCCAATAATGGTAAAAATCAAACTGAAATACTCAATGAATATGGAATGGCAAGGTCCACACTTCATAAA
TGGATAAAAGACTATAATAACTCAGGTTCATTCAGCGCTAAAGATAATAGATCTGATAAAGAAAAAGAATTAATTAAATTACAAAAAGAAAACAAGCAGT
TAAAAATGGAGAATGATATTTTAAAGCAAGCGGCGCTGATAATGGGACGAAAGTAGCAGTTATTAAGGCAAACAGGGATAAATACAGTATTAGCGCCATG
TGCAGAGCACTCAATATATCAAGGGGTATGATCTATTATACCCCTAAAGAAAAACAGGTTGATGTTGAACTAGAAAGCGAAGTGATTTCCATTTACAAAG
CAAGTAGAAATCACTATGGAACCAGAAAAATCAAAAGAGAATTAGCTAAAAAAGGTTATCAGGTGTCCAAGCGAAGAATAGGTAAAATAATGAAAAAATA
TAATCTAGTTTCTACTTATACTAAAAAACAATACAAAGTTCATTCTCCAAGCTGTAATGAAGATAAAATTGCCAATATTGTAAACAGAGAATTTAACAAA
GAAGAAGCTCTAGACGTTGTTGTCAGTGATTTAACCTATGTTAATGTAAAAGGAAAATGGAACTATGTCTGTCTGATCATAGATCTCTTCAACCGTGAAT
TTGTTGGTTATGCAGCAGGTAAAAAGAAAAATGCCGAATTAGTAACTGAGGCTTTTAAAAGTATTAAAAGACCACTAAATCAAATTAATATTTTACATAC
TGATAGAGGTAATGAATTCAAAAATAAAGCTATCGATGATATTTTAGTCAGGTTTGATATTGAGCGTTCTTTAAGCAATAAAGGATGCCCATATGATAAT
GCAGTGGCAGAAGCAGCCTTTAAAGTAGTTAAGACTGAATTTGCTTATGACAGAATATTTAACAGCTTTGAAGAGCTGGAATATGAGCTATTTGACTATG
TTAACTGGTATAATAACCACAGAATCCACGGGTCGTTAGATTACCTAACACCTGTTGAATATAGATATTTAATGTTCGATAAAAAATTGTTGTAAAAAGT
ATTGACAATCCA
TGAAGAAATCAAAAGAAAAGTTGTTGCTCTGGCCAATAATGGTAAAAATCAAACTGAAATACTCAATGAATATGGAATGGCAAGGTCCACACTTCATAAA
TGGATAAAAGACTATAATAACTCAGGTTCATTCAGCGCTAAAGATAATAGATCTGATAAAGAAAAAGAATTAATTAAATTACAAAAAGAAAACAAGCAGT
TAAAAATGGAGAATGATATTTTAAAGCAAGCGGCGCTGATAATGGGACGAAAGTAGCAGTTATTAAGGCAAACAGGGATAAATACAGTATTAGCGCCATG
TGCAGAGCACTCAATATATCAAGGGGTATGATCTATTATACCCCTAAAGAAAAACAGGTTGATGTTGAACTAGAAAGCGAAGTGATTTCCATTTACAAAG
CAAGTAGAAATCACTATGGAACCAGAAAAATCAAAAGAGAATTAGCTAAAAAAGGTTATCAGGTGTCCAAGCGAAGAATAGGTAAAATAATGAAAAAATA
TAATCTAGTTTCTACTTATACTAAAAAACAATACAAAGTTCATTCTCCAAGCTGTAATGAAGATAAAATTGCCAATATTGTAAACAGAGAATTTAACAAA
GAAGAAGCTCTAGACGTTGTTGTCAGTGATTTAACCTATGTTAATGTAAAAGGAAAATGGAACTATGTCTGTCTGATCATAGATCTCTTCAACCGTGAAT
TTGTTGGTTATGCAGCAGGTAAAAAGAAAAATGCCGAATTAGTAACTGAGGCTTTTAAAAGTATTAAAAGACCACTAAATCAAATTAATATTTTACATAC
TGATAGAGGTAATGAATTCAAAAATAAAGCTATCGATGATATTTTAGTCAGGTTTGATATTGAGCGTTCTTTAAGCAATAAAGGATGCCCATATGATAAT
GCAGTGGCAGAAGCAGCCTTTAAAGTAGTTAAGACTGAATTTGCTTATGACAGAATATTTAACAGCTTTGAAGAGCTGGAATATGAGCTATTTGACTATG
TTAACTGGTATAATAACCACAGAATCCACGGGTCGTTAGATTACCTAACACCTGTTGAATATAGATATTTAATGTTCGATAAAAAATTGTTGTAAAAAGT
ATTGACAATCCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 288 bp | 95 aa | 69 | 356 | + | No |
Description : First part of the transposase
ORF sequence :
MEENIMPTKYPEEIKRKVVALANNGKNQTEILNEYGMARSTLHKWIKDYNNSGSFSAKDNRSDKEKELIKLQKENKQLKMENDILKQAALIMGRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 879 bp | 292 aa | 317 | 1195 | + | No |
Chemistry : DDE
ORF sequence :
YFKASGADNGTKVAVIKANRDKYSISAMCRALNISRGMIYYTPKEKQVDVELESEVISIYKASRNHYGTRKIKRELAKKGYQVSKRRIGKIMKKYNLVST
YTKKQYKVHSPSCNEDKIANIVNREFNKEEALDVVVSDLTYVNVKGKWNYVCLIIDLFNREFVGYAAGKKKNAELVTEAFKSIKRPLNQINILHTDRGNE
FKNKAIDDILVRFDIERSLSNKGCPYDNAVAEAAFKVVKTEFAYDRIFNSFEELEYELFDYVNWYNNHRIHGSLDYLTPVEYRYLMFDKKLL
YTKKQYKVHSPSCNEDKIANIVNREFNKEEALDVVVSDLTYVNVKGKWNYVCLIIDLFNREFVGYAAGKKKNAELVTEAFKSIKRPLNQINILHTDRGNE
FKNKAIDDILVRFDIERSLSNKGCPYDNAVAEAAFKVVKTEFAYDRIFNSFEELEYELFDYVNWYNNHRIHGSLDYLTPVEYRYLMFDKKLL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1127 bp | 375 aa | 69 | 1195 | + | Yes |
Chemistry : DDE
ORF sequence :
MEENIMPTKYPEEIKRKVVALANNGKNQTEILNEYGMARSTLHKWIKDYNNSGSFSAKDNRSDKEKELIKLQKENKQLKMENDILKASGADNGTKVAVIK
ANRDKYSISAMCRALNISRGMIYYTPKEKQVDVELESEVISIYKASRNHYGTRKIKRELAKKGYQVSKRRIGKIMKKYNLVSTYTKKQYKVHSPSCNEDK
IANIVNREFNKEEALDVVVSDLTYVNVKGKWNYVCLIIDLFNREFVGYAAGKKKNAELVTEAFKSIKRPLNQINILHTDRGNEFKNKAIDDILVRFDIER
SLSNKGCPYDNAVAEAAFKVVKTEFAYDRIFNSFEELEYELFDYVNWYNNHRIHGSLDYLTPVEYRYLMFDKKLL
ANRDKYSISAMCRALNISRGMIYYTPKEKQVDVELESEVISIYKASRNHYGTRKIKRELAKKGYQVSKRRIGKIMKKYNLVSTYTKKQYKVHSPSCNEDK
IANIVNREFNKEEALDVVVSDLTYVNVKGKWNYVCLIIDLFNREFVGYAAGKKKNAELVTEAFKSIKRPLNQINILHTDRGNEFKNKAIDDILVRFDIER
SLSNKGCPYDNAVAEAAFKVVKTEFAYDRIFNSFEELEYELFDYVNWYNNHRIHGSLDYLTPVEYRYLMFDKKLL
Blast result :
Comments
ISHahy2 is 74% aa similar to IS655.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Frank R. (2016) Direct submission to ISfinder
2] Brown S.D., Begemann M.B., Mormile M.R., Wall J.D., Han C.S., Goodwin L.A., Pitluck S., Land M.L., Hauser L.J. and Elias D.A. (2011) J. Bacteriol. 193, 3682-3683
2] Brown S.D., Begemann M.B., Mormile M.R., Wall J.D., Han C.S., Goodwin L.A., Pitluck S., Land M.L., Hauser L.J. and Elias D.A. (2011) J. Bacteriol. 193, 3682-3683