ISfinder Mirror Site

ISPpu30

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP016215	ND	Pseudomonas putida	Pseudomonas putida Pseudomonas putida SY153 Pseudomonas aeruginosa PA121617 plasmid pBM413

DNA section

IS Length : 3000 bp

Ends

IR Length : 20/23

IRL : GTAAGCGTCTGGCGAACACACCTACAGAATCCGCAGATTAAGGGCGATGG
IRR : GTAAGCGTACGGCAAACACACCTTGCAAGCAGAACAGGCAATCAACGAGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGCCTCGAATGCC	TCAAGAGC	CGCGAAGTAGGAGC	8

DNA sequence

GTAAGCGTCTGGCGAACACACCTACAGAATCCGCAGATTAAGGGCGATGGTGTCCACCTAAAAATACGTGGACACCATCGCCCTTAATTCAAGGAACACC
ATGCGCCAACGCACCTCTTATCCCAAATCCTTCAAGGCTCAGGTTGTTCAGGAATGCCTGAACCCTGACGTATCAATTGCCAGCGTAGCCCTTCGGCACG
GCATCAACGCCAACCTGGTTCGGAAGTGGATACCCCTCTACCGTGACCCAAAAGTTTCTGCCTTACCTGCATTTGTACCGGTGAAGCTTGAGGCTGCGGC
GATGCCGGTTCGACAGGCTGTAGCTCGCATCGATATTTCTTCTGGGCATCAAACACTCACGGTGAGTTGGCCTGCCTCTGATCCAGACGGCTGCGCCCGC
TTCATTCGCAGCCTCTCGCAATGATCCGTATCGACGCCATCTGGCTCGCGACCGAGCCCATGGACATGCGTGCCGGCACGGATACAGCATTGGCCCGCGT
GGTCGCCGTATTCGGTGCGGCGAAGCCGCACTGCGCTTATCTGTTCGCCAACCGACGCGCCAACCGGATGAAAGTGCTGGTGCACGATGGCGTGGGCCTC
TGGCTGGCCGCGCGCCGCCTGAACCAAGGTAAATTCCATTGGCCCGGCACTCATCGAGGCCATGAAGTTGAACTCGATGCCGAACAACTCCAAGCGCTGG
TGCTGGGCCTGCCCTGGCAGCGGGTTGGCGCTAACGGCGCAATTACGTTGCTCTGAATCGGGTCTTTTAACTGTGACAGGTGGGCTGCTTTGGTAAAATC
CACGGCATGACTTCCTCGCCCAATCTCGACCAAATGACTCCCGATCAACTGCGCGCACTCGCTAAGCAGTTGCTGTCGAAGGTCGACACCATGGGCCAAG
AGAGCCGTCGCGACAAAACCATCATCGAGCAGCTCTCTCACGAGATCGCCATCCTCAAACGCCACAAGTTTGCCAAGCGCAGCGAGCAGATCAGCCCGGC
GCAAGGCAGCTTGCTGGATGACCTGCTCAGCACCGACCTCGAAGCCATCGACGCCGAGTTGAAAGCACTTCGTCCCGCACCAGCACCGGACGAACCACGC
CAACAACCCAAACGTGCGCCATTGCCGCCGCAGTTTCCTCGTACTGTCATTCATCATGAGCCAGAGAGCACCGAATGTACCTGCGGCTGCCAACTTCAGC
GCATCGGCGAGGATGTCAGTGAGAAGCTGGATTACACCCCGGGTGTGTTCACCGTTGAGCAACATGTACGTGGCAAATGGGCCTGCCGACAGTGCGAAAC
GCTGATCCAAGCGTCGGTGCCGGCACTGGTGATCGACAAGGGCATCCCTACTGCCGGCTTGCTGGCCCACGTGGTGGTGGCCAAGTTCGCCGATCATCTC
CCACTGTACCGGCAGGAAAAGATCTTTGGCCGTGCTGGCCTCGCTATCCCGCGCTCGACGCTTGCTCAGTGGGTCGGCCAAACCGGCCTACAACTGCAAC
CGTTGGTGGATGCACTGCGCGAGACAGTGCTGGCCCATCGAGTCGTCCATGCCGATGAAACACCGGTACAGATGCTGGCACCGGGTAAAAAGAAAACGCA
CCGAGCGTATGTCTGGGCCTACTGCACTACGCCGTTTTCGGCGTTGAAAGCTGTGGTTTACGACTTCAGCCCCAGCCGTGCTGGCGAACATGCGCGCAAC
TTCCTGGGCACTTGGAATGGCAAGCTGGTCTGCGATGACTTCGCAGGCTACAAGGCTGGCTTCGAACAGGGCATCACCGAAATCGGCTGCATGGCCCATG
CGCGGCGCAAGTTTTTCGACCTACATGTCGCGAACAAAAGCCAATTGGCAGAACAAGCGCTGCACTCCATCGGCGGCTTGTACGAAGTCGAACGGCAGGC
CAAAGGCATGAGCGATGAAAAGCGCTGGCGGTTACGCCAGCAAATAGCAGTGCCCATCGCCGAGAAACTGCATGAGTGGATGATGGCTCAGCGCGCGCTT
GTGCCCGAGGGCTCGGCTACGGCCAAGGCACTGGGTTACAGCCTGAAACGCTGGGTAGTGCTGACGCGCTACCTGGATGATGGTGCAGTGCCCATTGACA
ATAATGCAGTCGAAAACACGATCAGGCCGTGGGCGCTTGGGCGTTCCAACTGGCTCTTCGCTGGGTCACTGCGCAGTGGTAAACGGGCGGCGGCGATCAT
GAGCCTGATCCAGTCGGCGCGCATGAATGGGCATGATCCGTATATTTATCTGAAAGACGTTTTAGCGAGGTTGCCGACGCAACGAGCATCTAGCGTTGCG
CAACTGCTACCGCATCAATGGATTGCTGTATGACTCTCGGTAGGTATTCTCTGCTGAGTAAACTTTTCATTTGAAGAAAAATCGGAGCGTTTATGCGCCA
GGTTCTGCTCGTAGTCGATATTCAGTCCACATTCAACCCACCGGAATGGTTGGTTGACGGCGTGCAGGCGCTGTCGATGAAGATCCCGACAATTGCGTCT
ATCGAGCTTCACGATGAACAAGCGACGCCCTTCCAGCGTCAGCTCGGTTGGAGCCCGGCAAGCACTGACAAATGCCTTATCAAGGCCGACAGGGTCTTCG
TAAAAAATGGATATGGACAGACCTTAGAAACCATCAAGTACATCAAGGCGCTGGGCGTTGATCGAGTCCTGGTCTGCGGAATACAGACCGAAACCTGTGT
CCTCGCAGCTGGATTTGCATTGTTCGACGCTGGCCTCACTCCAACGCTGATAACGGATTTAACGGTGGGTTCGTCTTTGGATCGGTCTGGTCAACTCGGC
ATTGACCTGTGGGCACATCACTTCCGCAACGTGACAACGGCAGACGAAGTGATTGCAGGGCTGTCAGCGCTGCGTTGAAGGCCTGAGTAGCTCGATCAAG
AATCAACATCACCGATGTGCTCGCACTCCAAATTGGCAATATCCGAGCTGACTCGTTGATTGCCTGTTCTGCTTGCAAGGTGTGTTTGCCGTACGCTTAC

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
324 bp	107 aa	101	424	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MRQRTSYPKSFKAQVVQECLNPDVSIASVALRHGINANLVRKWIPLYRDPKVSALPAFVPVKLEAAAMPVRQAVARIDISSGHQTLTVSWPASDPDGCAR
FIRSLSQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
336 bp	111 aa	421	756	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRIDAIWLATEPMDMRAGTDTALARVVAVFGAAKPHCAYLFANRRANRMKVLVHDGVGLWLAARRLNQGKFHWPGTHRGHEVELDAEQLQALVLGLPWQ
RVGANGAITLL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1527 bp	508 aa	807	2333	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTSSPNLDQMTPDQLRALAKQLLSKVDTMGQESRRDKTIIEQLSHEIAILKRHKFAKRSEQISPAQGSLLDDLLSTDLEAIDAELKALRPAPAPDEPRQQ
PKRAPLPPQFPRTVIHHEPESTECTCGCQLQRIGEDVSEKLDYTPGVFTVEQHVRGKWACRQCETLIQASVPALVIDKGIPTAGLLAHVVVAKFADHLPL
YRQEKIFGRAGLAIPRSTLAQWVGQTGLQLQPLVDALRETVLAHRVVHADETPVQMLAPGKKKTHRAYVWAYCTTPFSALKAVVYDFSPSRAGEHARNFL
GTWNGKLVCDDFAGYKAGFEQGITEIGCMAHARRKFFDLHVANKSQLAEQALHSIGGLYEVERQAKGMSDEKRWRLRQQIAVPIAEKLHEWMMAQRALVP
EGSATAKALGYSLKRWVVLTRYLDDGAVPIDNNAVENTIRPWALGRSNWLFAGSLRSGKRAAAIMSLIQSARMNGHDPYIYLKDVLARLPTQRASSVAQL
LPHQWIAV

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
486 bp	161 aa	2393	2878	+	No

ORF function : Passenger Gene
Annotation : HydrolaseDescription :

ORF sequence :

MRQVLLVVDIQSTFNPPEWLVDGVQALSMKIPTIASIELHDEQATPFQRQLGWSPASTDKCLIKADRVFVKNGYGQTLETIKYIKALGVDRVLVCGIQTE
TCVLAAGFALFDAGLTPTLITDLTVGSSLDRSGQLGIDLWAHHFRNVTTADEVIAGLSALR

Blast result :

Comments

ISPpu30 is 78% (ORFA), 94% (ORFB) and 90% (ORFC : the transposase) aa similar to ISPpu14. ISPpu30 is disrupted by ISPpu29 (IS3 family member) and was reconstructed in silico by deletion of the ISPpu29 (IS3 family member)and of a copy of the direct repeat generated by ISPpu29 insertion (sequence GTA).

References

1] Dongsheng Zhou (2016) Direct submission.
2] Chen,D., Xu,Z. and Liu,J. (2016) Direct submission GenBank.