ISPpu29
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP016215 | ND | Pseudomonas putida | Pseudomonas putida Pseudomonas putida SY153 Pseudomonas aeruginosa strain PA121617 plasmid pBM413 |
DNA section
IS Length : 1425 bp
Ends
IR Length : 20/27
IRL : TATAGTGGATCCACCTTTTGAGACAGTGACTACGGGGTAGTTTAAGCTGT
IRR : TAAAGTGGACCCATCGGTCAAGACAGTTTTTCAGCGAGTTTAAGCTGCGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCATCCCTCA | GCC | ATTGCCTATG | 3 |
| CTCTCGGTAG | GTA | TTCTCTGCTG | 3 |
DNA sequence
TATAGTGGATCCACCTTTTGAGACAGTGACTACGGGGTAGTTTAAGCTGTCGTCCTCGAAAGGATGACAGCAAATGGGTGAAGCAAAGAAACGCAAGGTG
CACACGGCCGAATTCAAGGCCAAGGTAGGTCTGGAGGCGGTGCGCGGAGTGAAGACGATTACGGAGATTGCGCAGGCGTACGGCGTGCATCCGCAACTGG
TCGGGCAATGGAAGAAGGAGATCCTGGAATCGGCGGGCGCCCTGTTCGAGACGAAGCGCGGCCCGAAGCCAGCCGAGGACAAGAGCGGCGAAGAGCGGCT
GTACGGAGAAATCGGGCGGCTGAAGATGGAGAATGACTGGCTTAAAAAAAAGTTGGGAGTGTGAGCCTGGATGCGCGGGTCGGCTGGGTCGACGGCGCCG
ACAAGCTGTCCTTGAGCCGTCAGTGCGAGCTGGCCGAGGTGCCGCGCGCGACGGTCTACCGCAGGCTCACTGCCAAAGTGCCGGAAGAGGCGTGCGCCGA
GGATCTGCTGCTATGCCGCCTGATTGACGAGGAATACACGAGCAGACCGTTCTACGGCAGCCGGCGCATGGTGGTGTTCCTGCGGGGAGCAGGCCACGTT
GTCAACCGCAAGCGCGTGCAGCGCCTGATGCGCGGCATGGGCCTGGCGGGCATGGCGCCGGGGCCGAACACCAGCAAGCCGCAGCCGCAGCACAAGGTCT
ATCCGTATCTGCTGCGTGGCGTGGCGGTGACGCGGCCAAACCAGGTGTGGAGCACGGACATAACGTACATCAGGCTGGCGCGCGGTTTCGCTTACCTGGT
GGCGGTCATCGACTGGTACAGCCGGAAGGTGCTGTCCTGGCGGCTCAGCAACAGCATGGACGCATCGTTTTGCGTGGACTGCCTGGAAGACGCCCTGCGC
GAGCACGGCCGGCCCGAGGTGTTCAACAGCGACCAGGGATCGCAGTTTACCAGCGCGGCCTTCACGGGCGTGCTCAAACGCGAGGGCGTGGCTATCAGCA
TGGACGGGCGCGGACGGGCGCTGGACAACATCTTCGTCGAGCGCTTGTGGCGCAACGTGAAGCATGAGGACGTGTACCTGAAGGGCTACGCCAACATGGC
CGAGCTGACGGTGGGCCTGGCGCAGTACTTCGCGTTCTACAACGCCGAGCGGCCGCACCAGTCCCTTGGCTACAAGACACCTGCCAGCGTCTATCGCAGT
GGTATCGGCGGCGGCGCGCTGATCGCGGACAAGTACGGCGGCGCACAGCGGGAACTGGAAGCGGCGTGAACGCCGTCTGGCCTGCCGGCGGCGGCGGCCA
CCGGAGGGTAAGCTCTCCAGCGGCACGGCAGGAGAATCACGGGGCAGCGCCGACCAGCTGCAGTGGCAGTAAGCGACGCAGCTTAAACTCGCTGAAAAAC
TGTCTTGACCGATGGGTCCACTTTA
CACACGGCCGAATTCAAGGCCAAGGTAGGTCTGGAGGCGGTGCGCGGAGTGAAGACGATTACGGAGATTGCGCAGGCGTACGGCGTGCATCCGCAACTGG
TCGGGCAATGGAAGAAGGAGATCCTGGAATCGGCGGGCGCCCTGTTCGAGACGAAGCGCGGCCCGAAGCCAGCCGAGGACAAGAGCGGCGAAGAGCGGCT
GTACGGAGAAATCGGGCGGCTGAAGATGGAGAATGACTGGCTTAAAAAAAAGTTGGGAGTGTGAGCCTGGATGCGCGGGTCGGCTGGGTCGACGGCGCCG
ACAAGCTGTCCTTGAGCCGTCAGTGCGAGCTGGCCGAGGTGCCGCGCGCGACGGTCTACCGCAGGCTCACTGCCAAAGTGCCGGAAGAGGCGTGCGCCGA
GGATCTGCTGCTATGCCGCCTGATTGACGAGGAATACACGAGCAGACCGTTCTACGGCAGCCGGCGCATGGTGGTGTTCCTGCGGGGAGCAGGCCACGTT
GTCAACCGCAAGCGCGTGCAGCGCCTGATGCGCGGCATGGGCCTGGCGGGCATGGCGCCGGGGCCGAACACCAGCAAGCCGCAGCCGCAGCACAAGGTCT
ATCCGTATCTGCTGCGTGGCGTGGCGGTGACGCGGCCAAACCAGGTGTGGAGCACGGACATAACGTACATCAGGCTGGCGCGCGGTTTCGCTTACCTGGT
GGCGGTCATCGACTGGTACAGCCGGAAGGTGCTGTCCTGGCGGCTCAGCAACAGCATGGACGCATCGTTTTGCGTGGACTGCCTGGAAGACGCCCTGCGC
GAGCACGGCCGGCCCGAGGTGTTCAACAGCGACCAGGGATCGCAGTTTACCAGCGCGGCCTTCACGGGCGTGCTCAAACGCGAGGGCGTGGCTATCAGCA
TGGACGGGCGCGGACGGGCGCTGGACAACATCTTCGTCGAGCGCTTGTGGCGCAACGTGAAGCATGAGGACGTGTACCTGAAGGGCTACGCCAACATGGC
CGAGCTGACGGTGGGCCTGGCGCAGTACTTCGCGTTCTACAACGCCGAGCGGCCGCACCAGTCCCTTGGCTACAAGACACCTGCCAGCGTCTATCGCAGT
GGTATCGGCGGCGGCGCGCTGATCGCGGACAAGTACGGCGGCGCACAGCGGGAACTGGAAGCGGCGTGAACGCCGTCTGGCCTGCCGGCGGCGGCGGCCA
CCGGAGGGTAAGCTCTCCAGCGGCACGGCAGGAGAATCACGGGGCAGCGCCGACCAGCTGCAGTGGCAGTAAGCGACGCAGCTTAAACTCGCTGAAAAAC
TGTCTTGACCGATGGGTCCACTTTA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 291 bp | 96 aa | 74 | 364 | + | No |
Description : First part of the transposase
ORF sequence :
MGEAKKRKVHTAEFKAKVGLEAVRGVKTITEIAQAYGVHPQLVGQWKKEILESAGALFETKRGPKPAEDKSGEERLYGEIGRLKMENDWLKKKLGV
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 346 | 1269 | + | No |
Description : Second part of the transposase
ORF sequence :
KKVGSVSLDARVGWVDGADKLSLSRQCELAEVPRATVYRRLTAKVPEEACAEDLLLCRLIDEEYTSRPFYGSRRMVVFLRGAGHVVNRKRVQRLMRGMGL
AGMAPGPNTSKPQPQHKVYPYLLRGVAVTRPNQVWSTDITYIRLARGFAYLVAVIDWYSRKVLSWRLSNSMDASFCVDCLEDALREHGRPEVFNSDQGSQ
FTSAAFTGVLKREGVAISMDGRGRALDNIFVERLWRNVKHEDVYLKGYANMAELTVGLAQYFAFYNAERPHQSLGYKTPASVYRSGIGGGALIADKYGGA
QRELEAA
AGMAPGPNTSKPQPQHKVYPYLLRGVAVTRPNQVWSTDITYIRLARGFAYLVAVIDWYSRKVLSWRLSNSMDASFCVDCLEDALREHGRPEVFNSDQGSQ
FTSAAFTGVLKREGVAISMDGRGRALDNIFVERLWRNVKHEDVYLKGYANMAELTVGLAQYFAFYNAERPHQSLGYKTPASVYRSGIGGGALIADKYGGA
QRELEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1196 bp | 398 aa | 74 | 1269 | + | Yes |
Chemistry : DDE
ORF sequence :
MGEAKKRKVHTAEFKAKVGLEAVRGVKTITEIAQAYGVHPQLVGQWKKEILESAGALFETKRGPKPAEDKSGEERLYGEIGRLKMENDWLKKKVGSVSLD
ARVGWVDGADKLSLSRQCELAEVPRATVYRRLTAKVPEEACAEDLLLCRLIDEEYTSRPFYGSRRMVVFLRGAGHVVNRKRVQRLMRGMGLAGMAPGPNT
SKPQPQHKVYPYLLRGVAVTRPNQVWSTDITYIRLARGFAYLVAVIDWYSRKVLSWRLSNSMDASFCVDCLEDALREHGRPEVFNSDQGSQFTSAAFTGV
LKREGVAISMDGRGRALDNIFVERLWRNVKHEDVYLKGYANMAELTVGLAQYFAFYNAERPHQSLGYKTPASVYRSGIGGGALIADKYGGAQRELEAA
ARVGWVDGADKLSLSRQCELAEVPRATVYRRLTAKVPEEACAEDLLLCRLIDEEYTSRPFYGSRRMVVFLRGAGHVVNRKRVQRLMRGMGLAGMAPGPNT
SKPQPQHKVYPYLLRGVAVTRPNQVWSTDITYIRLARGFAYLVAVIDWYSRKVLSWRLSNSMDASFCVDCLEDALREHGRPEVFNSDQGSQFTSAAFTGV
LKREGVAISMDGRGRALDNIFVERLWRNVKHEDVYLKGYANMAELTVGLAQYFAFYNAERPHQSLGYKTPASVYRSGIGGGALIADKYGGAQRELEAA
Blast result :
Comments
There are 4 copies of ISPpu29 in the plasmid of Pseudomonas putida SY153.
The transposase of ISPpu29 is 79% aa similar to ISAfe17.
The transposase of ISPpu29 is 79% aa similar to ISAfe17.
References
1] Zhou D. (2016) Direct submission