ISSpn11

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Streptococcus pneumoniae
Streptococcus pneumoniae
DNA section
IS Length : 1436 bp

Ends


IR Length : 23/25

IRL : TGAACTGCACCCCAAAAGTTAGACAGAAAAAATCTAACTTTTGGGGTGTT
IRR : TGTACTGCCCCCCAAAAGTTAGACAATTAATTTATCCGAAGGATTTAGTT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGAACTGCACCCCAAAAGTTAGACAGAAAAAATCTAACTTTTGGGGTGTTTTTATTATGAAATTAAGTTATGATGATAAAGTTCAGATCTATGAACTTAG
AAAACAAGGATATAGCTTAGAGAAGCTTTCAAATAAATTTGAGATAAACAATTCTAATATTAGGTACATGATTAAATTGATTGATCGTTACGGAATAGAG
TTCGTCAAAAAAGGAAAAAATCGTTACTATTCTCCTGATTTAAAACAAGAAATGATTAATAAAGTCTTACATGAAGGCTGGACTAAAGATAGAGTTTCTC
TTGAATACGGTCTCCCAAGTCGTACGATACTTCTTAACTGGCTAGCACAATACAGGAAAAACGGGTATACTATTGTTGAGAAACCAAGAGGGAGAGTACC
TAAAATGGGACGTAAGCCAAGAACGAGACCTGAAGAGAGGACAGAATTAGAACGTCTTCAAGCAGAAAATGAGTACCTGAGAGCGGAGAATGCCATCCTA
AAAAAGTTAAGAGAACTCCGATTGAAGGAGGAAAAAGAGAAAGAAGAAAGACAGAAATTGTTCAAGAATTAATGACTGAGTTTTCGTTAGTTCTTCTTCT
AAAAGCCATTAAACTAGCTCGTTGGACCTACTACTATCACTTGAAACAGCTAGACAAAACAGATAAGGACCAAGAGCTTAAAGCTGAAATTCAATCCATT
TTTATCGAACACAAAGGAAATTATGCTTATCGCCGGGTTCATTTAGAACTAAGAAATCGTGCTTATCTGGTAAATCATAAAAGAGTTCAAGGCTTGATAA
AAGTACTCAATTTACAAGCTAAAATGCGACAGAAACGAAAATATTCTTCTCATAAAAGAGACGTTGGCAAGAAGGCAGAGAATCTCATTCAAGGCCAACT
TGAAGGCTCTAAAACAATGGAAAAGTGCTACACAGATGTGACAGAATTTGCCATTCCAGCAAGTACTCAAAAGCTTTACTTATCACCAGTTTTAGATGGC
TTTAACAGCGAAATTATCGCCTATAATCTTTCTTGTTCGCCTAATTTAGAACAAGTACAAACAATGTTGGAACAGGCATTCAAAGAGAAGCACTATGAGA
ATACGATTCTCCATAGTGACCAAGGCTGTCAATACCAACACGATTCTTATCATCGGTTCCTAGAGAGTAAGGGAATTCAAGCATCCATGTCACGCAAGGG
AAACAGCCAAGACCACGGTATGATGGAATCTTTCTTTGGCATTTTAAAATCCGAAATGTTTTATGGCTATGAGAAAACATTTAAATCACTTAATCAATTG
GAACAAGCCATTATAGACTATATTGATTATTACAACAACAAGAGAATTAAGGTAAAACTAAAAGGACTTAGCCCTGTGCAATACAGAACTAAATCCTTCG
GATAAATTAATTGTCTAACTTTTGGGGGGCAGTACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 2
LengthBeginEndStrandFusion ORF
933 bp310 aa4731405+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

VPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKAIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLV
NHKRVQGLIKVLNLQAKMRQKRKYSSHKRDVGKKAENLIQGQLEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSCSPNLEQVQTMLE
QAFKEKHYENTILHSDQGCQYQHDSYHRFLESKGIQASMSRKGNSQDHGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLS
PVQYRTKSFG

 

Blast result :
ORF 1
LengthBeginEndStrandFusion ORF
516 bp171 aa57572+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MKLSYDDKVQIYELRKQGYSLEKLSNKFEINNSNIRYMIKLIDRYGIEFVKKGKNRYYSPDLKQEMINKVLHEGWTKDRVSLEYGLPSRTILLNWLAQYR
KNGYTIVEKPRGRVPKMGRKPRTRPEERTELERLQAENEYLRAENAILKKLRELRLKEEKEKEERQKLFKN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1349 bp449 aa571405+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKLSYDDKVQIYELRKQGYSLEKLSNKFEINNSNIRYMIKLIDRYGIEFVKKGKNRYYSPDLKQEMINKVLHEGWTKDRVSLEYGLPSRTILLNWLAQYR
KNGYTIVEKPRGRVPKMGRKPRTRPEERTELERLQAENEYLRAENAILKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKAIKLARWTYYYHLKQLD
KTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSHKRDVGKKAENLIQGQLEGSKTMEKCYTDVTEFAI
PASTQKLYLSPVLDGFNSEIIAYNLSCSPNLEQVQTMLEQAFKEKHYENTILHSDQGCQYQHDSYHRFLESKGIQASMSRKGNSQDHGMMESFFGILKSE
MFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG

 

Blast result :
Comments
ISSpn11 has very similar termini to ISSpn4, but highly divergent central segment.
ISSpn11 is 87% aa similar to ISSpn4. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift.

Please could this entry be kept confidential until the paper is published.
References
[1] Croucher, N.J., Coupland, P.G., Stevenson, A.E., Callendrello, A., Bentley, S.D. and Hanage W.P. (2014) Nat. Commun. 5:5471