ISCaje3
- Family IS607
- Group
Isoform Synonym(s) ISCaje2
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_027997 | ND | Campylobacter sp. | Campylobacter sp. phage CP220 Campylobacter sp. phage CPt10 |
DNA section
IS Length : 1653 bp
Ends
IR Length : 49/65
IRL : TATATAATATATGAAATATTATAAGAATATATAAATATCTATAATTATGT
IRR : CCAACTAAACTATTAAAAAAATATTATAAAGAACTATAAAAATTCTTTAC
Comments : IRs are not at the termini.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTGTGTTTTTAT | TTCTTT | AAGTATTTGACA | 6 |
DNA sequence
TATATAATATATGAAATATTATAAGAATATATAAATATCTATAATTATGTATAATTTTTCTTAAAAAGACTTTAAATGTATATTATTGGAGAATTTTCTA
GAATTATAGGTGTTACTAAGACAACCTTAAGAAATTAATGAGAAAAACTATATAATTTATATCAAATATTATATATTCTGGACTTAAAAGTATATTAAAC
TAAATAAATATAGAAATTATTTTAGAATTTTCAAAAATTGAAAGTTTTGAAATTTAAATATAATAAAACGAGATTTAAAAGAATTCCAGTTCTTTTAAAT
CTCTAATTTTAAACATAGGTATAATATGTCTAAAACTAAAAATATTTATAGCACTGTTAGAAGTGTTAAATTTGTATTAAAGCCTAACATTAAACAAAGA
GAGATACTTGAATCATTCTTTGGGTTATCTAGGACTATATACAATATTTCTTTACATAATATAAAGAATTCAAAATTTGGAACTTATGAAATTCAAAATG
GTAAAAACAAAGGTTGTATAGTTCCAAGAATTCCAACTGAGTTTGATTTGAACAATTCTATACCTAAATTAAAAGATGGATACAGCTTTTTATCTTTATT
ACCAGCTACTTACACCCAATCTGTAATGAAAAATCTTAGCAGGGGATTTAATAATTTTTACAGAACTTTCAATTATCCTAAATTTAAAGCTAAAAAGAAC
AGTATTCAAAGCTTTAATTGTTATGCTGGAGCAAAAATAGAAGGTGATCATATAATTCTAATAAAACCAAGATGTTCTGATTACACTAAAGAAGATTTAA
AGATAAGATTTAAAAGACATAAGATAAAATATAATTTTGATAAGGTGACTGGATTTACCATTTCAAAAGAAAATAATAAGTATTTTATCTCTTTCACTTT
TCATTGTAATATAGAAAGTAAAGAAACATCAGATGCGGTTGGTATTGATCTAGGTATTAAAGATTTTTGTATTTGTAGCGATGGAACAATATATGAAAAT
AAAAGATTTCTTGAAAAATCACTAAGAAAATTAAAGATTTCTCAAAGAAAGTTATCAAAGAAACAAAAGGGTTCTAATAATAGAGAAAAACAAAGATTAA
AAGTTTCAAAGCTTCATAAAAAAGTGAAGAACCAAAGAAATGACTACCAACACAAAGTCTCAAGAGAGATAGCTGATAAGTATAGAACTATTTGCCTTGA
GACTTTAAAAGTCAAGAATATGGTTAAGAATAAAAAACTGGCTAAAGCAATATCTGATGTTAGTTGGAGTTCTTTCATAGAAAAATTAAGTTATAAAGTA
GCCGAAAACCAAGGCTGTTTAATTAAGATTGATACGCACTACCCTAGTAGTAGAACCTGTTCTAATTGTGGTTGTATTAAAGAAAATTTAAGTTTATCTG
AACGAACCTATAATTGCGAAGAATGTGGATTTAGTATTGATAGAGATTTAAATGCTAGTATCAATATTTTAAATCTTGGATTAAAGAATATAGTGGCTAC
TGTAACAACTACAGGAACCACTCTAAAAAATTAAAGCTTGTGGAGTTCTTAAACAACGCTAGAAATAGAGAAGGACAATGAAGCAAGAAAAACTATTAAA
AATGTAAAGAATTTTTATAGTTCTTTATAATATTTTTTTAATAGTTTAGTTGG
GAATTATAGGTGTTACTAAGACAACCTTAAGAAATTAATGAGAAAAACTATATAATTTATATCAAATATTATATATTCTGGACTTAAAAGTATATTAAAC
TAAATAAATATAGAAATTATTTTAGAATTTTCAAAAATTGAAAGTTTTGAAATTTAAATATAATAAAACGAGATTTAAAAGAATTCCAGTTCTTTTAAAT
CTCTAATTTTAAACATAGGTATAATATGTCTAAAACTAAAAATATTTATAGCACTGTTAGAAGTGTTAAATTTGTATTAAAGCCTAACATTAAACAAAGA
GAGATACTTGAATCATTCTTTGGGTTATCTAGGACTATATACAATATTTCTTTACATAATATAAAGAATTCAAAATTTGGAACTTATGAAATTCAAAATG
GTAAAAACAAAGGTTGTATAGTTCCAAGAATTCCAACTGAGTTTGATTTGAACAATTCTATACCTAAATTAAAAGATGGATACAGCTTTTTATCTTTATT
ACCAGCTACTTACACCCAATCTGTAATGAAAAATCTTAGCAGGGGATTTAATAATTTTTACAGAACTTTCAATTATCCTAAATTTAAAGCTAAAAAGAAC
AGTATTCAAAGCTTTAATTGTTATGCTGGAGCAAAAATAGAAGGTGATCATATAATTCTAATAAAACCAAGATGTTCTGATTACACTAAAGAAGATTTAA
AGATAAGATTTAAAAGACATAAGATAAAATATAATTTTGATAAGGTGACTGGATTTACCATTTCAAAAGAAAATAATAAGTATTTTATCTCTTTCACTTT
TCATTGTAATATAGAAAGTAAAGAAACATCAGATGCGGTTGGTATTGATCTAGGTATTAAAGATTTTTGTATTTGTAGCGATGGAACAATATATGAAAAT
AAAAGATTTCTTGAAAAATCACTAAGAAAATTAAAGATTTCTCAAAGAAAGTTATCAAAGAAACAAAAGGGTTCTAATAATAGAGAAAAACAAAGATTAA
AAGTTTCAAAGCTTCATAAAAAAGTGAAGAACCAAAGAAATGACTACCAACACAAAGTCTCAAGAGAGATAGCTGATAAGTATAGAACTATTTGCCTTGA
GACTTTAAAAGTCAAGAATATGGTTAAGAATAAAAAACTGGCTAAAGCAATATCTGATGTTAGTTGGAGTTCTTTCATAGAAAAATTAAGTTATAAAGTA
GCCGAAAACCAAGGCTGTTTAATTAAGATTGATACGCACTACCCTAGTAGTAGAACCTGTTCTAATTGTGGTTGTATTAAAGAAAATTTAAGTTTATCTG
AACGAACCTATAATTGCGAAGAATGTGGATTTAGTATTGATAGAGATTTAAATGCTAGTATCAATATTTTAAATCTTGGATTAAAGAATATAGTGGCTAC
TGTAACAACTACAGGAACCACTCTAAAAAATTAAAGCTTGTGGAGTTCTTAAACAACGCTAGAAATAGAGAAGGACAATGAAGCAAGAAAAACTATTAAA
AATGTAAAGAATTTTTATAGTTCTTTATAATATTTTTTTAATAGTTTAGTTGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1209 bp | 402 aa | 326 | 1534 | + | No |
AG : TnpB
ORF sequence :
MSKTKNIYSTVRSVKFVLKPNIKQREILESFFGLSRTIYNISLHNIKNSKFGTYEIQNGKNKGCIVPRIPTEFDLNNSIPKLKDGYSFLSLLPATYTQSV
MKNLSRGFNNFYRTFNYPKFKAKKNSIQSFNCYAGAKIEGDHIILIKPRCSDYTKEDLKIRFKRHKIKYNFDKVTGFTISKENNKYFISFTFHCNIESKE
TSDAVGIDLGIKDFCICSDGTIYENKRFLEKSLRKLKISQRKLSKKQKGSNNREKQRLKVSKLHKKVKNQRNDYQHKVSREIADKYRTICLETLKVKNMV
KNKKLAKAISDVSWSSFIEKLSYKVAENQGCLIKIDTHYPSSRTCSNCGCIKENLSLSERTYNCEECGFSIDRDLNASINILNLGLKNIVATVTTTGTTL
KN
MKNLSRGFNNFYRTFNYPKFKAKKNSIQSFNCYAGAKIEGDHIILIKPRCSDYTKEDLKIRFKRHKIKYNFDKVTGFTISKENNKYFISFTFHCNIESKE
TSDAVGIDLGIKDFCICSDGTIYENKRFLEKSLRKLKISQRKLSKKQKGSNNREKQRLKVSKLHKKVKNQRNDYQHKVSREIADKYRTICLETLKVKNMV
KNKKLAKAISDVSWSSFIEKLSYKVAENQGCLIKIDTHYPSSRTCSNCGCIKENLSLSERTYNCEECGFSIDRDLNASINILNLGLKNIVATVTTTGTTL
KN
Blast result :
Comments
ISCaje3 is 53% aa (TnpB) similar to IS607.
In the first part of the IS, there is a remnant of the serine transposase (IS607 family).
For ISCaje2, the 53 last nucleotides are missing. ISCaje2 and ISCaje3 are 93% aa similar.
In the first part of the IS, there is a remnant of the serine transposase (IS607 family).
For ISCaje2, the 53 last nucleotides are missing. ISCaje2 and ISCaje3 are 93% aa similar.
References
1] ISfinder submission (2016)
2] Timms,A.R., Cambray-Young,J., Scott,A.E., Petty,N.K., Connerton,P.L., Clarke,L., Seeger,K., Quail,M., Cummings,N., Maskell,D.J., Thomson,N.R. and Connerton,I.F. (2010) BMC Genomics 11, 214.
2] Timms,A.R., Cambray-Young,J., Scott,A.E., Petty,N.K., Connerton,P.L., Clarke,L., Seeger,K., Quail,M., Cummings,N., Maskell,D.J., Thomson,N.R. and Connerton,I.F. (2010) BMC Genomics 11, 214.