ISCaje1
- Family IS607
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_027997 | ND | Campylobacter sp. | Campylobacter sp. phage CP220 Campylobacter sp. phage CPt10 |
DNA section
IS Length : 1515 bp
Ends
IR Length : 18/22
IRL : -TTCTATGCCATCTATGAAATATTATAAGAATATATAAATATCTATAATT
IRR : GATTTTTAAATTCTATAGAAATCTACAAGAATTTAATCAAATATTACAAA
Comments : IRs are not at the termini of the sequence.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACATTCATTTT | ATA | TATTTTTGTA | 3 |
DNA sequence
TTCTATGCCATCTATGAAATATTATAAGAATATATAAATATCTATAATTATATTATAAGTTTTCTTAAAAAGGATTTAAATGTATAGTATTGGAGAATTT
TCTAGAATTATAGGTGTTACTAGGACAACCTTAAGAAATTGGGATAAAACAAATAAACTAAAACCTATTTTACTCGAATCGGGCCATAGAAGAGAAATAT
AAATAGATATAAAGAAAGGAGTTTAGAATGTTGTCTAGAAAGATAAGAATTGATAACTTTATCTCTGATAAAGAAAAAGAAGAAATATATCAATATTCTA
AATCTCTTTCTGCTATCTACAACGAATGTCTAGATCTTCTTAAAGAAAATTTGAATTTTAAAGATCTATCTAAAATCACAAAAGGTGGATCAAAAGCAAC
AGGCTTGCATTCAAAACATATACAGAATACCTCTAGAGAGGTTATAAATGCTGTAAAATCTTATCTAGTGAAAAAGAAGAACGATAAGTCTGCTAGATTT
CCAAAATTACATAGAGAATATAGTCCTATCATTATGGTCGCAAATCTTTCCTCTAAGACGGAGGAGATTGTTAACTCTAGAACTGGAGAAGCGATTCTCG
AAAAGAAATACTATCCAGGTGGAGGATTTAAACTAGAAGGTAAGAAAATAAACTTTACTTCAATAGGATTTGAATTAGATTTAAGTAAATGCCCTTACTA
TGATATAGAACTTATCAACTATGAGACTTTAAAACAGATAGTCATCAAGATCGATGAGAATAAGAGAATAGATTGTATTTTTGTTTTCTCAGAGAAAAAA
CAAGAAAAAGCACCAAATCAAAATTTTCTTTCCATAGATCTAGGAATAAGCAGTATAGCATCTTGTTACTCAAACAAGATTGATTGCTTGAAGATACAAA
CTAAGAGATTTAAAGGTCTAGAAAGAACTATAAATGAGCTGAAGTCTAAAAGAGATAAGAAGAAAAAAGACTCAAGAGCATATAAGAAACTTAACAAAAC
AATCAGAAGAAAGCAAGCAAAACTAACTAATAAAAGAAAAGACTATCTTCATAAGGCCTCAAAGACTGTAGTAGATCTCTGTATTCTTAATGGTATAGAT
AACATCATTTGTGGAGATATCAAGACTAAAAAATTAAAGAAAGACTATAAAACAAGTTTAAACAAATCAACCCAAAATGAAGGACTATTGAGTAGATTTA
AGGGTTTCTTAAAGTATAAAGCAGAGAATAAAGGATTGAACTTTTTACTTGTGAATGAAGCATATACTTCTCAGACTAACTGCCTTACAGGAAAAAGAGA
GCTAGACTCGAATCTCAGTATCAGAGAGGTAGAATTAAGTCCAGGTTTCAAAGTTGATAGAGATATAAATTCAGCTGTCAATATAGCCAAAATATGTGGG
GATTTATGGTTATCCCATATCTTTGAGAAGAATAGACTTCTCAAGATACAAAAAATGAATATTACTTTGTAATATTTGATTAAATTCTTGTAGATTTCTA
TAGAATTTAAAAATC
TCTAGAATTATAGGTGTTACTAGGACAACCTTAAGAAATTGGGATAAAACAAATAAACTAAAACCTATTTTACTCGAATCGGGCCATAGAAGAGAAATAT
AAATAGATATAAAGAAAGGAGTTTAGAATGTTGTCTAGAAAGATAAGAATTGATAACTTTATCTCTGATAAAGAAAAAGAAGAAATATATCAATATTCTA
AATCTCTTTCTGCTATCTACAACGAATGTCTAGATCTTCTTAAAGAAAATTTGAATTTTAAAGATCTATCTAAAATCACAAAAGGTGGATCAAAAGCAAC
AGGCTTGCATTCAAAACATATACAGAATACCTCTAGAGAGGTTATAAATGCTGTAAAATCTTATCTAGTGAAAAAGAAGAACGATAAGTCTGCTAGATTT
CCAAAATTACATAGAGAATATAGTCCTATCATTATGGTCGCAAATCTTTCCTCTAAGACGGAGGAGATTGTTAACTCTAGAACTGGAGAAGCGATTCTCG
AAAAGAAATACTATCCAGGTGGAGGATTTAAACTAGAAGGTAAGAAAATAAACTTTACTTCAATAGGATTTGAATTAGATTTAAGTAAATGCCCTTACTA
TGATATAGAACTTATCAACTATGAGACTTTAAAACAGATAGTCATCAAGATCGATGAGAATAAGAGAATAGATTGTATTTTTGTTTTCTCAGAGAAAAAA
CAAGAAAAAGCACCAAATCAAAATTTTCTTTCCATAGATCTAGGAATAAGCAGTATAGCATCTTGTTACTCAAACAAGATTGATTGCTTGAAGATACAAA
CTAAGAGATTTAAAGGTCTAGAAAGAACTATAAATGAGCTGAAGTCTAAAAGAGATAAGAAGAAAAAAGACTCAAGAGCATATAAGAAACTTAACAAAAC
AATCAGAAGAAAGCAAGCAAAACTAACTAATAAAAGAAAAGACTATCTTCATAAGGCCTCAAAGACTGTAGTAGATCTCTGTATTCTTAATGGTATAGAT
AACATCATTTGTGGAGATATCAAGACTAAAAAATTAAAGAAAGACTATAAAACAAGTTTAAACAAATCAACCCAAAATGAAGGACTATTGAGTAGATTTA
AGGGTTTCTTAAAGTATAAAGCAGAGAATAAAGGATTGAACTTTTTACTTGTGAATGAAGCATATACTTCTCAGACTAACTGCCTTACAGGAAAAAGAGA
GCTAGACTCGAATCTCAGTATCAGAGAGGTAGAATTAAGTCCAGGTTTCAAAGTTGATAGAGATATAAATTCAGCTGTCAATATAGCCAAAATATGTGGG
GATTTATGGTTATCCCATATCTTTGAGAAGAATAGACTTCTCAAGATACAAAAAATGAATATTACTTTGTAATATTTGATTAAATTCTTGTAGATTTCTA
TAGAATTTAAAAATC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1245 bp | 414 aa | 228 | 1472 | + | No |
AG : TnpB
ORF sequence :
MLSRKIRIDNFISDKEKEEIYQYSKSLSAIYNECLDLLKENLNFKDLSKITKGGSKATGLHSKHIQNTSREVINAVKSYLVKKKNDKSARFPKLHREYSP
IIMVANLSSKTEEIVNSRTGEAILEKKYYPGGGFKLEGKKINFTSIGFELDLSKCPYYDIELINYETLKQIVIKIDENKRIDCIFVFSEKKQEKAPNQNF
LSIDLGISSIASCYSNKIDCLKIQTKRFKGLERTINELKSKRDKKKKDSRAYKKLNKTIRRKQAKLTNKRKDYLHKASKTVVDLCILNGIDNIICGDIKT
KKLKKDYKTSLNKSTQNEGLLSRFKGFLKYKAENKGLNFLLVNEAYTSQTNCLTGKRELDSNLSIREVELSPGFKVDRDINSAVNIAKICGDLWLSHIFE
KNRLLKIQKMNITL
IIMVANLSSKTEEIVNSRTGEAILEKKYYPGGGFKLEGKKINFTSIGFELDLSKCPYYDIELINYETLKQIVIKIDENKRIDCIFVFSEKKQEKAPNQNF
LSIDLGISSIASCYSNKIDCLKIQTKRFKGLERTINELKSKRDKKKKDSRAYKKLNKTIRRKQAKLTNKRKDYLHKASKTVVDLCILNGIDNIICGDIKT
KKLKKDYKTSLNKSTQNEGLLSRFKGFLKYKAENKGLNFLLVNEAYTSQTNCLTGKRELDSNLSIREVELSPGFKVDRDINSAVNIAKICGDLWLSHIFE
KNRLLKIQKMNITL
Blast result :
Comments
ISCaje1 is 51% aa similar to ISCbo10.
There are empty site in Campylobacter phage CPt10 (FN667789.1) and Campylobacter phage CP21 (HE815464.1).
In the first part of the IS, there is a remnant of the serine transposase (IS607 family).
There are empty site in Campylobacter phage CPt10 (FN667789.1) and Campylobacter phage CP21 (HE815464.1).
In the first part of the IS, there is a remnant of the serine transposase (IS607 family).
References
1] ISfinder submission (2016)
2] Timms,A.R., Cambray-Young,J., Scott,A.E., Petty,N.K., Connerton,P.L., Clarke,L., Seeger,K., Quail,M., Cummings,N., Maskell,D.J., Thomson,N.R. and Connerton,I.F. (2010) BMC Genomics 11, 214.
2] Timms,A.R., Cambray-Young,J., Scott,A.E., Petty,N.K., Connerton,P.L., Clarke,L., Seeger,K., Quail,M., Cummings,N., Maskell,D.J., Thomson,N.R. and Connerton,I.F. (2010) BMC Genomics 11, 214.