ISDeth1
- Family IS5
- Group IS903
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP002543 | ND | Desulfurobacterium thermolithotrophum | Desulfurobacterium thermolithotrophum DSM 11699 |
DNA section
IS Length : 980 bp
Ends
IR Length : 14/15
IRL : GAGGGTGTTTGAAAATTGCCAAAATTAAGAAAAAAGCCTATCCTCTCCGG
IRR : GAGAGTGTTTGAAAAATCATCCAGACAAAGAACAAAACTCCCATGTTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAAGCATA | GTACAGTGAT | TGGAAAAGCT | 10 |
| GAATTTGGCA | ATATCCTAAA | AAAGAGAGAT | 10 |
| GATTATCCCA | AAGGGGGGAA | TATGATTAAA | 10 |
| GTTTTTTGGCA | AGGTTTTAA | AAATTTTTTTT | 9 |
| CCTAACAGGAA | AATCCAATC | AGAAATTCAAC | 9 |
| TGTTAAGTTT | GAATATGAACT | GTTGAAGTTC | 11 |
| GAATTTGTCA | AGGGGTTTAGG | GAAATTTTAA | 11 |
| TCCTGTTCCT | ATCTATGCAG | TAACACATGA | 9 |
| AGCCATGTCA | ACTATTTAAA | GAGAAACTTT | 9 |
| AATAACTTGA | CAACTAAAGT | TGACAACAAT | 9 |
| TCAACAGCCA | TGTCTAAAATT | TTCAGAAGCA | 11 |
| CTTTTTTCTCATTGCTTTGT | AGTTCATTAA AATGAAACAA | 0 | |
| ACATTCCACA AGTTCATTAA | GAGAGAAACTTGATTCTACG | 0 | |
| AGAAGAAGAAAGAAGCTGAA | ATTTAAAAGGTATTAAATTT | 0 | |
| CTTTTCTTTCTTAAGGTTAT | TCTTAAGGTTATTGACTTCC | 0 | |
| AAGTAATTAATTGTAGCAAA | AGCTTGTATTCCTTACTTAA | 0 | |
| CCAATGTCAAGTTCTTTAAA | AGTTCTTTAAATAATAAACA | 0 | |
| TTCGTATGTCAAGCTTGTAT | CTTGCTTTGTCTCCTTAAAT | 0 | |
| AAACGTTGAGAACATAGTAG | CACAGAAGCTTGACAAAGTC | 0 | |
| ATTTTAGCAAGTATTTATTT | GGAGTTTGATTAGTTTCTCT | 0 |
DNA sequence
GAGGGTGTTTGAAAATTGCCAAAATTAAGAAAAAAGCCTATCCTCTCCGGTATGAAAGCGAAAAGACTAAATTTCCACAAAGTACTCAACCTATCAAAAA
CATACATGAACCGGAGAGGACAGGCTGTACTGGTATATTTATATCCTTTTAAAACCAAAAGAGGAAGACCCAAGAAATACCCTGACGAGATAATTCTTAC
CCTTCTTTTCCTCCAAGTAGCCTGGAACCTATCATTCAGAGACCTTGAATATTTGGCAGTTCAGATATTTGGAAGAGAGAATATTCCTGATTTTTCAACC
TATTATTACCGACTCAAGCAACTACCTTCCATTCTCCTTGTAGATTTTCTGAACTTTGTCTCTCGAAGACTCTTAGGGAAGTATCATAAAGAACTAAGAT
TTCTGATAATAGATGGAACTGGCTTCAAATACAATGAGATTTATCCATTGAAAATTCTAAGAGGCAAAGAGATAAAGGAGGTAAAAAGCCACGTTAAAGT
TGTTGTATTAAGCGTTCATCTAAAAGATGGAAAAAGGTTCATTCTTACTGCATTACCTGGAGAGAGTTACGCTTCGGAAGTGAAACTTGGAGAGAAAATA
GTTAGGTGGTTAAACGAAAGGGGATTTATATGGAGAGCTTTGAAAGGAAAGCCATTTTTAGGAGACAAAGCTTATGACAGCATTAAGTTTATTGAGCTTG
TTCTGTTAGTAGGCTTAAAGCCTTACATAAAGGTGAGAGAAACATTAAGGAAGGGAATTAAATCTGAGATTAGGCTTAAATGCAAAGAGCTTTTAGAATC
TGATGAAATTTACAGGTTTAGAGGACTGATAGAGAGTATTTTTGGAGAAGTTAAGCAGGATGTTGGAAGTTACGAGAAAACAAAGAGCTTTCATATAGCT
CAACTGTTTGTTTTAGCTAAGTTTATCCTCTTTAACATGGGAGTTTTGTTCTTTGTCTGGATGATTTTTCAAACACTCTC
CATACATGAACCGGAGAGGACAGGCTGTACTGGTATATTTATATCCTTTTAAAACCAAAAGAGGAAGACCCAAGAAATACCCTGACGAGATAATTCTTAC
CCTTCTTTTCCTCCAAGTAGCCTGGAACCTATCATTCAGAGACCTTGAATATTTGGCAGTTCAGATATTTGGAAGAGAGAATATTCCTGATTTTTCAACC
TATTATTACCGACTCAAGCAACTACCTTCCATTCTCCTTGTAGATTTTCTGAACTTTGTCTCTCGAAGACTCTTAGGGAAGTATCATAAAGAACTAAGAT
TTCTGATAATAGATGGAACTGGCTTCAAATACAATGAGATTTATCCATTGAAAATTCTAAGAGGCAAAGAGATAAAGGAGGTAAAAAGCCACGTTAAAGT
TGTTGTATTAAGCGTTCATCTAAAAGATGGAAAAAGGTTCATTCTTACTGCATTACCTGGAGAGAGTTACGCTTCGGAAGTGAAACTTGGAGAGAAAATA
GTTAGGTGGTTAAACGAAAGGGGATTTATATGGAGAGCTTTGAAAGGAAAGCCATTTTTAGGAGACAAAGCTTATGACAGCATTAAGTTTATTGAGCTTG
TTCTGTTAGTAGGCTTAAAGCCTTACATAAAGGTGAGAGAAACATTAAGGAAGGGAATTAAATCTGAGATTAGGCTTAAATGCAAAGAGCTTTTAGAATC
TGATGAAATTTACAGGTTTAGAGGACTGATAGAGAGTATTTTTGGAGAAGTTAAGCAGGATGTTGGAAGTTACGAGAAAACAAAGAGCTTTCATATAGCT
CAACTGTTTGTTTTAGCTAAGTTTATCCTCTTTAACATGGGAGTTTTGTTCTTTGTCTGGATGATTTTTCAAACACTCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 965 bp | 321 aa | 16 | 980 | + | No |
Chemistry : DDE
ORF sequence :
MPKLRKKPILSGMKAKRLNFHKVLNLSKTYMNRRGQAVLVYLYPFKTKRGRPKKYPDEIILTLLFLQVAWNLSFRDLEYLAVQIFGRENIPDFSTYYYRL
KQLPSILLVDFLNFVSRRLLGKYHKELRFLIIDGTGFKYNEIYPLKILRGKEIKEVKSHVKVVVLSVHLKDGKRFILTALPGESYASEVKLGEKIVRWLN
ERGFIWRALKGKPFLGDKAYDSIKFIELVLLVGLKPYIKVRETLRKGIKSEIRLKCKELLESDEIYRFRGLIESIFGEVKQDVGSYEKTKSFHIAQLFVL
AKFILFNMGVLFFVWMIFQTL
KQLPSILLVDFLNFVSRRLLGKYHKELRFLIIDGTGFKYNEIYPLKILRGKEIKEVKSHVKVVVLSVHLKDGKRFILTALPGESYASEVKLGEKIVRWLN
ERGFIWRALKGKPFLGDKAYDSIKFIELVLLVGLKPYIKVRETLRKGIKSEIRLKCKELLESDEIYRFRGLIESIFGEVKQDVGSYEKTKSFHIAQLFVL
AKFILFNMGVLFFVWMIFQTL
Blast result :Comments : The STOP codon is after the end of the transposase, so the end of the transposase was in silico deleted.
Comments
ISDeth1 is 55% aa similar to ISSulsp1.
References
1] ISfinder annotation (2016)
2] Goker,M., Daligault,H., Mwirichia,R., Lapidus,A., Lucas,S., Deshpande,S., Pagani,I., Tapia,R., Cheng,J.F., Goodwin,L., Pitluck,S., Liolios,K., Ivanova,N., Mavromatis,K., Mikhailova,N., Pati,A., Chen,A., Palaniappan,K., Han,C., Land,M., Hauser,L., Pan,C., Brambilla,E.M., Rohde,M., Spring,S., Sikorski,J., Wirth,R., Detter,J.C., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. (2011) Stand Genomic Sci 5 (3), 407-415.
2] Goker,M., Daligault,H., Mwirichia,R., Lapidus,A., Lucas,S., Deshpande,S., Pagani,I., Tapia,R., Cheng,J.F., Goodwin,L., Pitluck,S., Liolios,K., Ivanova,N., Mavromatis,K., Mikhailova,N., Pati,A., Chen,A., Palaniappan,K., Han,C., Land,M., Hauser,L., Pan,C., Brambilla,E.M., Rohde,M., Spring,S., Sikorski,J., Wirth,R., Detter,J.C., Woyke,T., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. (2011) Stand Genomic Sci 5 (3), 407-415.