ISNfl1

  • Family ISNCY
  • Group ISDol1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
HQ291166 ND Nostoc flagelliforme
Nostoc flagelliforme Sunitezuoqi
DNA section
IS Length : 2022 bp

Ends


IR Length : 30/32

IRL : CTGGAGTTTAGACTAGTTTCCGGTGCGAAACTTCAAACCTTTACAGCGAT
IRR : CTGGAGTTTAGACTAGTTCTCGGTGCGAAACTGCTGAACAAATCAGATAT

Insertion site


Left flankDirect repeatRight flankDR Length
ATCAGTAGCGCTAAAAAGGTTTGATGTATAACTGCATC0

DNA sequence

CTGGAGTTTAGACTAGTTTCCGGTGCGAAACTTCAAACCTTTACAGCGATTGATATACATCAAATATAAGCGCATCCACCCAAAACTTGAGTAGGGTTTT
CATAGTACGTTTGAACTAAAAATGCCCCAGGCACTCAAGGATGCGATCGCGTTCCGTAAGCTGAAGCATCCTTGAGTGCGTTGTAGGCGATGAACCTCCG
GTGCTGTGGAGGCGATTGCGCGGAGCGCAGTGCTGGGGGCAATCGCCAGAAAAAAATTGACGAGGGCGATTTTCTTGTAATCAAGTAAGAGAGATAAAAA
TGATGACTGAATTTCAATGAAAAATAAATTTTTTTGAACTATTTTTGAGTAAATTAAAATGGTTAATCGTTCTGAAGTATGTTTTTGAGTAGTACTTTCT
TTGCAAGCTCACTTAAAAAGCGATGTTACGTCAAAAATATGAATGTGACAAAGAGTTTAGTATTCTGATTCAGTTAATAGGAGAGATGGATGATTCAATT
TATATAATAGATAAAGTCCTTTCGGATGAACATCTGTTTCGATTAATTGAGTCTGATTTATCAAAGCGTTATCAGAACACTACTAAAACTGGAAGGAAAT
CAACTCCGGTTGAAGTTGTATTACGAATGTTAGCCTTGAAGCACTTACGCGGTTTGAGCTACGAGCAAACAATTTCCAACGTAAATGAAAGTTTAATTCT
AAGGCAATTTTGTCGAATTTATTTTAATGCTCTTCCTAATAAAAGCACTTTGATTCGTTGGTCACATCAAATTCGTCAAGAAACCTTGTCACAATTTAAT
CAACGTCTGACTCAAGTTGCGACCCAATTACAAATAACTAAGGGTAGAAAGATGAGGACAGATGGTACAGTAGTCGCAACCAATATTCATTTTCCTTCTG
ATAACAGCTTGTTACTTGATGGAGTCAAAGTTATTAGTCGTTTTTTATTGCAGGCAAAAGAAATTAGTGTCGCCAATTCGATATTTATTAACTCAAGTCT
TTTTCGTAATCGTTATCGTACAGCTAGAAGAATTTCTAGAGAGATTGATGGTTTATCTAAAACGAGAAATCAATCTGGCCGTCAAAAAAGAGAAAAAGCC
TACTCCAAATTGATTGAAGTGACTCAAGCTAGTTGGAAACAGGCTCAAAAATTCAAGTCAATTATTGGTAATTTAAATTTACTACAGTTTCATCAGCTAC
TTCAAAGATTTGAAATTTTTCTCCCTCGTATATCACAAGTGATTGAACAAACTCAGAGACGAATTTTCAATTCGGAGAAAGTACCTGCTGCCGAAAAAAT
TGTCAGTATATTTTGAATCTCATACAGATATAATATGTCGAGGTAAAATCAATGTAGATGTGGAATTTGGACATAAAGTTTGGTTAGATGAAATTGACGG
TGGAATTGTCAGCAATTATCGAATTTTAAAGGGTAATCCTCATGATACTCAACAATTAATCCCAAGTCTTGACCAACATTTAGAAAATTTTGGTTCATCG
CCAAAAATAGTGACTACAGACAGAGGTGTTTACTCTCAAACAAACGAAAATTATGCTCAATTATTAGGAGTCAAAGAAGTCATCTTACCCAAGGGTGGTT
ATCGAAGTAAAGAACGAATCAAGCATGAAAGAAAAAGAAATTTTAAAAAAGCTCGTCGTTGGCATAATGGAGTAGAAGGACGTATCAGTTTTTTGAAACG
ATGTTTTGGCTTACAACGTTGTTTATATAGGGGAGAGTTAGGATTTTGTCGTTGGGTTGGCTGGGGAGTTATTGCTCATAATTTAACTATTATTAGTCGA
GGGACTCTCAAAAATAAAATAAATTATCATGTTTTTATTTGATTTTATTATCAATAAAATTTGAGTTTAATCAAATATTACACTATCAGGAATAGTAGCT
TAAATACTTGAAATGAGCTTTGCTCTCATAATATAGTTGGCACATGCAACTATTGATTATTAACAAAGTTTTATATCTGATTTGTTCAGCAGTTTCGCAC
CGAGAACTAGTCTAAACTCCAG
Recoding section
  • Recoding by frameshift
  • Frame +1
  • Type transcriptional
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence : No
  • Secondary structure : No-structure

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
894 bp297 aa4231316+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MLRQKYECDKEFSILIQLIGEMDDSIYIIDKVLSDEHLFRLIESDLSKRYQNTTKTGRKSTPVEVVLRMLALKHLRGLSYEQTISNVNESLILRQFCRIY
FNALPNKSTLIRWSHQIRQETLSQFNQRLTQVATQLQITKGRKMRTDGTVVATNIHFPSDNSLLLDGVKVISRFLLQAKEISVANSIFINSSLFRNRYRT
ARRISREIDGLSKTRNQSGRQKREKAYSKLIEVTQASWKQAQKFKSIIGNLNLLQFHQLLQRFEIFLPRISQVIEQTQRRIFNSEKVPAAEKIVSIF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
600 bp199 aa12431842+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LNKLRDEFSIRRKYLLPKKLSVYFESHTDIICRGKINVDVEFGHKVWLDEIDGGIVSNYRILKGNPHDTQQLIPSLDQHLENFGSSPKIVTTDRGVYSQT
NENYAQLLGVKEVILPKGGYRSKERIKHERKRNFKKARRWHNGVEGRISFLKRCFGLQRCLYRGELGFCRWVGWGVIAHNLTIISRGTLKNKINYHVFI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1420 bp473 aa4231842+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLRQKYECDKEFSILIQLIGEMDDSIYIIDKVLSDEHLFRLIESDLSKRYQNTTKTGRKSTPVEVVLRMLALKHLRGLSYEQTISNVNESLILRQFCRIY
FNALPNKSTLIRWSHQIRQETLSQFNQRLTQVATQLQITKGRKMRTDGTVVATNIHFPSDNSLLLDGVKVISRFLLQAKEISVANSIFINSSLFRNRYRT
ARRISREIDGLSKTRNQSGRQKREKAYSKLIEVTQASWKQAQKFKSIIGNLNLLQFHQLLQRFEIFLPRISQVIEQTQRRIFNSEKVPAAEKKLSVYFES
HTDIICRGKINVDVEFGHKVWLDEIDGGIVSNYRILKGNPHDTQQLIPSLDQHLENFGSSPKIVTTDRGVYSQTNENYAQLLGVKEVILPKGGYRSKERI
KHERKRNFKKARRWHNGVEGRISFLKRCFGLQRCLYRGELGFCRWVGWGVIAHNLTIISRGTLKNKINYHVFI

 

Blast result :
Comments
ISNfl1 is 53% aa similar to ISTco5.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
References
1] ISfinder annotation (2016)
2] Gao,X., Liu,K. and Qiu,B.S. (2011) Acta Physiol. Plant. 33 (4), 1301-1318.