ISDph1

  • Family ISNCY
  • Group ISDol1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
APJX01000004 ND Desulfotignum phosphitoxidans
Desulfotignum phosphitoxidans DSM 13687
DNA section
IS Length : 1882 bp

Ends


IR Length : 14/18

IRL : GTGTGTGACCGAAAACTCAAAACCCCACGTTTTTCATGGGTATAATGGCT
IRR : GTGGCTGACCGGAAACCCCTATTTTAGGATTTTCCGAGGCCGATGCCTGA

Insertion site


Left flankDirect repeatRight flankDR Length
ATTCCCAACTACCTATATTAGCAATGGCTTC7

DNA sequence

GTGTGTGACCGAAAACTCAAAACCCCACGTTTTTCATGGGTATAATGGCTAAAATTTGTTTGAAATCTTTACTTGAAATTGAATTACTTGTTGATTTTAA
CTGATTACTATGTTAATATAACCAATTAATAATTAAAGAGATTTTAAAAAAACTGAGAGTCATAGAAAGCAGTTCAAATTTCAACACCCCAACAAAACTT
CGAGGTTGTTTTAGCGTAAAATTTTGAACCTACAAATGACATTCGGGCAGACCCCTATCGACCAAATCAAACTTGACATGAGAGCCAGGGATGAAATTCC
CAAGCTACTTTTGGGGCTCCAGCATATCTATTGCGATCAAGAACTTCGAGAAAAAGTTTTTGTCATCCTCAAAAATGTGATTCCGGAAGACACCGACTCC
AAAAATGGACGTCCGGGAATGGACCTGTGGAAAATTCTTGTGATGGGCACCATACGGCTCAATTGCAATTGGGATTACGATAAACTCCGGGAGATGGTGA
ACAACCACAGAACATTGAGGCAGATGCTGGGTCATGGCATGATGGACGATGACATAACCTATCCGCTCCAGACCTTAAAAGATAATGTCAGGCTTCTGAC
ACCTGACATTCTTGATAAAATCAACACCCTGGTTGTACAAGAAGGTCATAAACTTCTGATGAAAAAAAAACTTCGGACGAAGAGGTGTTGATGGGACGGT
GTGATTCATTCGTTGTTGAAACCGATGTTCATTTTCCCACAGACATCAACCTGCTTTTTGATGCAGTCCGAAAAATGATTCAAATTGCCGCTATTATCAG
CCAGGGCATTGGAACGAGTATGTGGCGGCAATCAGCATATAATATCAAAAAATTTAAACGGCTGTATCGGATAGTTCAGCGATTGAAACATTCCACATCC
GCGGATGAAAAGAAAAAGGCCAAAAGAGCCCGGCAGATCATGGATGCACACAAAGCTTATATTGAACTTGCTGAAAAATACATTTCAAAAGCGGAATTGA
CCATTGAAATGACGGAGTCCTCCGATATTATGAATGAAGCCCGAGTGGAAGAGTTGCAAACATACATCAATTATGCGCTTTGGCAAATTGGCCTGATAAA
ACGCCGGGTTTTGCAGGATGAAAAGATCCCACATAAAGACAAGATTTTTTCAATTTTCGAACCCCATACAGAATGGATTTCAAAAGGCAAAGCCGGTGTT
CCCCAAGAATTGGGACTGCGGGTATGCATCCTGGAAGACCAGTATGGGTTTATCCTGCACCACCGGGTGATGGAGCAAGAAACCGATGATAAAGTGGCCG
TTGCAATGGTAACGTCGGCCCAAAACAAATTTTCTGGTCTCAAGGGATGCAGTTTTGATAAAGGGTTTTATACACCTGGTAACAAAAAGGACCTGAAGGA
TACATTGAGCATTTTGGTGCTCCCGAAAAAAGGCAGATGCAACAAGGCTGAATTTGAAGAAGAAACAGCAGACGATTTTATTCGTTTAAAAAGAAAACAT
TCAGCGGTGGAATCGGCCATAAACGGGTTGGAAAATCATGGTCTGGACAGATGCCCGGATCATGGCATTCAAGGATTTAAAAGATATGTAGGTCTGTCTG
TTCTGGCAAGAAATCTCCAGATTATGGGTCATCATATACAACAAAAAAGGCTGAAGCAGCTGCAACGGTCTGAACAGCGAAAAGCCGCGTAAAAAAAGGC
CGTCGCATCAAAAAGTAGCACGTCAAACACTACAGGTGTGTCCAAAAAATGGGAAAGTCGTTAAAAAACGACAAATATTGAAGCACTTTGCCAGGTTTGT
GATAGCAGGAAAAACATTAATTGGAAATTATGTCAGGCATCGGCCTCGGAAAATCCTAAAATAGGGGTTTCCGGTCAGCCAC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type transcriptional
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence : No
  • Secondary structure : No-structure

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
456 bp151 aa236691+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTFGQTPIDQIKLDMRARDEIPKLLLGLQHIYCDQELREKVFVILKNVIPEDTDSKNGRPGMDLWKILVMGTIRLNCNWDYDKLREMVNNHRTLRQMLGH
GMMDDDITYPLQTLKDNVRLLTPDILDKINTLVVQEGHKLLMKKKLRTKRC

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1041 bp346 aa6521692+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TSDEKKTSDEEVLMGRCDSFVVETDVHFPTDINLLFDAVRKMIQIAAIISQGIGTSMWRQSAYNIKKFKRLYRIVQRLKHSTSADEKKKAKRARQIMDAH
KAYIELAEKYISKAELTIEMTESSDIMNEARVEELQTYINYALWQIGLIKRRVLQDEKIPHKDKIFSIFEPHTEWISKGKAGVPQELGLRVCILEDQYGF
ILHHRVMEQETDDKVAVAMVTSAQNKFSGLKGCSFDKGFYTPGNKKDLKDTLSILVLPKKGRCNKAEFEEETADDFIRLKRKHSAVESAINGLENHGLDR
CPDHGIQGFKRYVGLSVLARNLQIMGHHIQQKRLKQLQRSEQRKAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1457 bp484 aa2361692+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTFGQTPIDQIKLDMRARDEIPKLLLGLQHIYCDQELREKVFVILKNVIPEDTDSKNGRPGMDLWKILVMGTIRLNCNWDYDKLREMVNNHRTLRQMLGH
GMMDDDITYPLQTLKDNVRLLTPDILDKINTLVVQEGHKLLMKKTSDEEVLMGRCDSFVVETDVHFPTDINLLFDAVRKMIQIAAIISQGIGTSMWRQSA
YNIKKFKRLYRIVQRLKHSTSADEKKKAKRARQIMDAHKAYIELAEKYISKAELTIEMTESSDIMNEARVEELQTYINYALWQIGLIKRRVLQDEKIPHK
DKIFSIFEPHTEWISKGKAGVPQELGLRVCILEDQYGFILHHRVMEQETDDKVAVAMVTSAQNKFSGLKGCSFDKGFYTPGNKKDLKDTLSILVLPKKGR
CNKAEFEEETADDFIRLKRKHSAVESAINGLENHGLDRCPDHGIQGFKRYVGLSVLARNLQIMGHHIQQKRLKQLQRSEQRKAA

 

Blast result :Comments : This ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Comments
ISDph1 is 72% aa similar to ISDssp5.
References
1] ISfinder annotation (2016)
2] Poehlein,A., Daniel,R. and Simeonova,D.D. (2013) Genome Announc 1 (3), E00227-13.