ISKpn34

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP008932 ND Klebsiella pneumoniae
Klebsiella pneumoniae plasmid pNDM-MAR
Citrobacter freundii plasmid pNDM-CIT
Klebsiella pneumoniae strain PMK1 plasmid pPMK1-C
Klebsiella pneumoniae strain PMK1 plasmid pPMK1-NDM
Salmonella enterica strain F8025 plasmid pF8025
Escherichia coli strain Ecol_732 plasmid pEC732_IMP14
Citrobacter amalonaticus Y19
DNA section
IS Length : 1221 bp

Ends


IR Length : 33/45

IRL : TGACCTGCTCCCCGTTGATTAATACACCGTGATGTTAGTAATGTCTTCAT
IRR : TGACCTGCCCCCAGAGTTAGATACAACCTTCAGTTAGTAATGTCGGTTGG

Insertion site


Left flankDirect repeatRight flankDR Length
CTAACAGGAACTCGCAATAC0
TTACATAGCTCATTATTTTATAGT4
GTTCTTGGTGGAAGTTATGCGACT4
CCCTACGCGACATC(g/c)CTTAATATAAA6
TGTCGTTAATGCTCATTTATACTC4
GCTGCCTGAGTAGGAGCTATAACA4

DNA sequence

TGACCTGCTCCCCGTTGATTAATACACCGTGATGTTAGTAATGTCTTCATAAGCCACATGAGGACATCCCCATGAAGAAGCGTTTTTCCGACGAACAGAT
CATATGTATTCTCCGCGAGGCCGAAGCCGGCGTTTCTGCCCGTGAGCTCTGCCGTAAGCACGCCATTTCCGACGCCACCTTTTACACATGGCGTAAGAAG
TATGGCGGTATGGAGGTGCCTGAGGTTAAGCGCCTGAAGTCGCTTGAGGAAGAGAACGCCAGACTCAAGAAGCTGCTTGCTGAAGCCATGCTGGATAAGG
AGGCGCTTCAGGTGGCTCTTGGGCGAAAGTACTGACGACAGACCAGAAGCGGGAAGCCGTGGAAGTCATGTGCGAGGCTAAGGGTCTGTCGCAACGTCGT
GCCTGCAGGCTGGCAGGTCTGTCCCTGTCAACCTGCCGATATTCGGCTCAGCGTCCGGCTGCTGACGCGCAGCTGTCTCTACGCATCACAGAGCTGGCAC
TTGAACGCCGCCGTTTTGGTTACCGGCGTATCTGGCAGCTTCTGCGACGTGAAGGTCTTTGCGTTAACCACAAGCGGGTTTACCGCATCTATCAACTTAA
TGGCCTGAGTGTAAAACGCAGACGACGTCGTAAAGGGCTGGCAACAGAACGTCTGCCGCTGCTCCGCCCGATGGCGCCCAATCTGACCTGGTCAATGGAT
TTCGTCATGGACGCACTGGCCACAGGTCGCAGGATCAAGTGCCTGACCTGCGTGGATGATTTCACAAAGGAATGCCTGACGGTCACTGTTGCCTTCGGGA
TTTCAGGCGTGCAGGTCACGCGTATTCTGGACAGCATTGCGCTGTTTCGCGGCTATCCGGCTATGATAAGAACCGATCAGGGCCCGGAGTTTACCTGCCG
CGCACTCGATCAGTGGGCTTTTGAGCATGGTGTGGAGCTGCGACTTATCCAGCCCGGCAAGCCAACGCAGAACGGATTTATTGAGAGTTTTAACGGACGC
TTTCGTGATGAATGCCTGAATGAGCACTGGTTCAGCGATATTGTTCACGCCAGGAAGATCATTAATGACTGGCGACTGGATTATAACGAGTGTCGACCAC
ATTCATCACTGAATTACCTGACGCCGGCTGAATTTGCAGCGGGCTGGCGAAACGGGAAATATGAAGAAAAACCAACCGACATTACTAACTGAAGGTTGTA
TCTAACTCTGGGGGCAGGTCA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
264 bp87 aa72335+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKRFSDEQIICILREAEAGVSARELCRKHAISDATFYTWRKKYGGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKY

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
894 bp297 aa2991192+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GGASGGSWAKVLTTDQKREAVEVMCEAKGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELALERRRFGYRRIWQLLRREGLCVNHKRVYRIYQL
NGLSVKRRRRRKGLATERLPLLRPMAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPAMIRTDQGPEFTC
RALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRLDYNECRPHSSLNYLTPAEFAAGWRNGKYEEKPTDITN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1121 bp373 aa721192+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKRFSDEQIICILREAEAGVSARELCRKHAISDATFYTWRKKYGGMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKVLTTDQKREAVEVM
CEAKGLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELALERRRFGYRRIWQLLRREGLCVNHKRVYRIYQLNGLSVKRRRRRKGLATERLPLLRP
MAPNLTWSMDFVMDALATGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPAMIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQ
NGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRLDYNECRPHSSLNYLTPAEFAAGWRNGKYEEKPTDITN

 

Blast result :
Comments
ISKpn34 is 95% aa similar to ISSpr2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
[1] ISfinder (2016) Direct submission