ISPa57
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LN870292 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa DK1 Pseudomonas aeruginosa PA7 |
DNA section
IS Length : 1323 bp
Ends
IR Length : 26/28
IRL : TGTCCCGTCCTGAATAAGGTTTACACCTCCCATGCCGATTTTCCGGAGAG
IRR : TGTCTAGTCCTGAAATAGGTTTACACCTGCTCAGGTAGGCCGATATGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTTCGTTTC | TAC | CGTTCGTCAG | 0 |
| TTTTCATCAA | GGA | GTCTCAGCGC | 0 |
| TTTTTCATCT | GCT | GCTCGCCGAC | 0 |
| TTTTCATTCC | CGG | CGGCGCTGGC | 0 |
DNA sequence
TGTCCCGTCCTGAATAAGGTTTACACCTCCCATGCCGATTTTCCGGAGAGCTAGATGGAGCAAGGTGTAAAGCGCACACAGCGAGATTACTCACTGTCTT
TTAAATTGGCCGTCGTTGATCAGATCGAAAAAGGCGAACTGACCTACAAGCAGGCCCAGGTGCGGTATGGCATCCAGGGCCGGTCGACGGTTCTGGTATG
GTTGCGCAAGCATGGTCGGCAGGATTGGAGCCAAGGGGCGTCGATTCGAGCCGAGAGGAGTTGCGCGATGTCTGACCCCAAAACGCTCACTCCAGAGCAG
CGGATCAAGGAGCTTGAGCAGCAGCTTGAGTTGATGAGCCAGAAGGCTCAGTTCTTCGAGACCGTCGTTGATGTCCTGAAAAATGATTACGGTGTATCGG
TCGTAAAAAAGCGATCCGGCAAGTCCTCTCGCAAGGTCAAGTCGCAGGACTGAGCATTGCTAGGGCTTGTCAGTTTCTTGGCATGAGCCGACAGGCTTAC
TACAAGCGCAATCGAGCTGCTGATAGCAAAGCACGCCAGGCAGATCGGGTTGTCGAATTTGTTCAACAAGTCCGGATGCGTCAGCCACGACTAGGCACGC
GAAAGCTGCACTATCTGCTGCATTGCCAACCTGATAGGCAGCTCCAAGTTGGCCGAGACCGACTATTTCAGGTCCTGGGAGAGCGCCGTTTGCTGGTGTT
GCCCAAGCGGGCGTATCACAAGACAACACAAAGCTTTCATCGCTTCTACCGCCATCCGAACCTACTCAAGCCAGGCCCGAGCCAGGTTGTCCCGTCAGGA
CCGGAGCACGTTTGGGTGGCGGACATTACTTACTTGCCAGCCAGTAGTGGCCCGCTGTACCTAAGCCTGGTAACGGATGCGTATTCCAGAAAAATTGTTG
GCCACCATGTGCATGAAAGCCTGCATGCCGAGTCAGTTGCACAAGCCTTTAGGCAGGCCCTACGCAAACGGCAGCGTCGCCAGTCACTGGTCCATCACTC
GGATCGCGGTATTCAATATTGCTCGGCACTGTATCAGTCACTGCATGCACGGCATGGCGTCCAGTGCTCGATGACTGACGGATATGACTGCTACCAGAAC
GCGCTAGCTGAGCGCATCAACGGAATCCTCAAAACAGAGCTGCTGCTGAGTAGCCCTGAAAATCTGGAGCAGGCAAGGAAGATGGTTGCTGAAGCCGTGC
AGATCTACAACACAGAGCGGCCTCACATGGCCCTGAAAAACAAAACGCCCGAGACGGTACATCGGGCGTTTTGAGGCATATCGGCCTACCTGAGCAGGTG
TAAACCTATTTCAGGACTAGACA
TTAAATTGGCCGTCGTTGATCAGATCGAAAAAGGCGAACTGACCTACAAGCAGGCCCAGGTGCGGTATGGCATCCAGGGCCGGTCGACGGTTCTGGTATG
GTTGCGCAAGCATGGTCGGCAGGATTGGAGCCAAGGGGCGTCGATTCGAGCCGAGAGGAGTTGCGCGATGTCTGACCCCAAAACGCTCACTCCAGAGCAG
CGGATCAAGGAGCTTGAGCAGCAGCTTGAGTTGATGAGCCAGAAGGCTCAGTTCTTCGAGACCGTCGTTGATGTCCTGAAAAATGATTACGGTGTATCGG
TCGTAAAAAAGCGATCCGGCAAGTCCTCTCGCAAGGTCAAGTCGCAGGACTGAGCATTGCTAGGGCTTGTCAGTTTCTTGGCATGAGCCGACAGGCTTAC
TACAAGCGCAATCGAGCTGCTGATAGCAAAGCACGCCAGGCAGATCGGGTTGTCGAATTTGTTCAACAAGTCCGGATGCGTCAGCCACGACTAGGCACGC
GAAAGCTGCACTATCTGCTGCATTGCCAACCTGATAGGCAGCTCCAAGTTGGCCGAGACCGACTATTTCAGGTCCTGGGAGAGCGCCGTTTGCTGGTGTT
GCCCAAGCGGGCGTATCACAAGACAACACAAAGCTTTCATCGCTTCTACCGCCATCCGAACCTACTCAAGCCAGGCCCGAGCCAGGTTGTCCCGTCAGGA
CCGGAGCACGTTTGGGTGGCGGACATTACTTACTTGCCAGCCAGTAGTGGCCCGCTGTACCTAAGCCTGGTAACGGATGCGTATTCCAGAAAAATTGTTG
GCCACCATGTGCATGAAAGCCTGCATGCCGAGTCAGTTGCACAAGCCTTTAGGCAGGCCCTACGCAAACGGCAGCGTCGCCAGTCACTGGTCCATCACTC
GGATCGCGGTATTCAATATTGCTCGGCACTGTATCAGTCACTGCATGCACGGCATGGCGTCCAGTGCTCGATGACTGACGGATATGACTGCTACCAGAAC
GCGCTAGCTGAGCGCATCAACGGAATCCTCAAAACAGAGCTGCTGCTGAGTAGCCCTGAAAATCTGGAGCAGGCAAGGAAGATGGTTGCTGAAGCCGTGC
AGATCTACAACACAGAGCGGCCTCACATGGCCCTGAAAAACAAAACGCCCGAGACGGTACATCGGGCGTTTTGAGGCATATCGGCCTACCTGAGCAGGTG
TAAACCTATTTCAGGACTAGACA
Recoding section
- Recoding by frameshift
- Frame +1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 399 bp | 132 aa | 55 | 453 | + | No |
Description : First part of the transposase
ORF sequence :
MEQGVKRTQRDYSLSFKLAVVDQIEKGELTYKQAQVRYGIQGRSTVLVWLRKHGRQDWSQGASIRAERSCAMSDPKTLTPEQRIKELEQQLELMSQKAQF
FETVVDVLKNDYGVSVVKKRSGKSSRKVKSQD
FETVVDVLKNDYGVSVVKKRSGKSSRKVKSQD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 888 bp | 295 aa | 387 | 1274 | + | No |
Description : Second part of the transposase
ORF sequence :
LRCIGRKKAIRQVLSQGQVAGLSIARACQFLGMSRQAYYKRNRAADSKARQADRVVEFVQQVRMRQPRLGTRKLHYLLHCQPDRQLQVGRDRLFQVLGER
RLLVLPKRAYHKTTQSFHRFYRHPNLLKPGPSQVVPSGPEHVWVADITYLPASSGPLYLSLVTDAYSRKIVGHHVHESLHAESVAQAFRQALRKRQRRQS
LVHHSDRGIQYCSALYQSLHARHGVQCSMTDGYDCYQNALAERINGILKTELLLSSPENLEQARKMVAEAVQIYNTERPHMALKNKTPETVHRAF
RLLVLPKRAYHKTTQSFHRFYRHPNLLKPGPSQVVPSGPEHVWVADITYLPASSGPLYLSLVTDAYSRKIVGHHVHESLHAESVAQAFRQALRKRQRRQS
LVHHSDRGIQYCSALYQSLHARHGVQCSMTDGYDCYQNALAERINGILKTELLLSSPENLEQARKMVAEAVQIYNTERPHMALKNKTPETVHRAF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 55 | 1274 | + | Yes |
Chemistry : DDE
ORF sequence :
MEQGVKRTQRDYSLSFKLAVVDQIEKGELTYKQAQVRYGIQGRSTVLVWLRKHGRQDWSQGASIRAERSCAMSDPKTLTPEQRIKELEQQLELMSQKAQF
FETVVDVLKNDYGVSVVKKAIRQVLSQGQVAGLSIARACQFLGMSRQAYYKRNRAADSKARQADRVVEFVQQVRMRQPRLGTRKLHYLLHCQPDRQLQVG
RDRLFQVLGERRLLVLPKRAYHKTTQSFHRFYRHPNLLKPGPSQVVPSGPEHVWVADITYLPASSGPLYLSLVTDAYSRKIVGHHVHESLHAESVAQAFR
QALRKRQRRQSLVHHSDRGIQYCSALYQSLHARHGVQCSMTDGYDCYQNALAERINGILKTELLLSSPENLEQARKMVAEAVQIYNTERPHMALKNKTPE
TVHRAF
FETVVDVLKNDYGVSVVKKAIRQVLSQGQVAGLSIARACQFLGMSRQAYYKRNRAADSKARQADRVVEFVQQVRMRQPRLGTRKLHYLLHCQPDRQLQVG
RDRLFQVLGERRLLVLPKRAYHKTTQSFHRFYRHPNLLKPGPSQVVPSGPEHVWVADITYLPASSGPLYLSLVTDAYSRKIVGHHVHESLHAESVAQAFR
QALRKRQRRQSLVHHSDRGIQYCSALYQSLHARHGVQCSMTDGYDCYQNALAERINGILKTELLLSSPENLEQARKMVAEAVQIYNTERPHMALKNKTPE
TVHRAF
Blast result :
Comments
ISPa57 is 86% identical to ISAs31.
IS_PEP 3 is the putative OrfAB transposase reconstructred in silico by a possible +1 frameshift.
IS_PEP 3 is the putative OrfAB transposase reconstructred in silico by a possible +1 frameshift.
References
[1] Anders Norman (2015) Direct submission