ISPpu23

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KU130294 ND Pseudomonas putida
Pseudomonas putida 12969 plasmid p12969-DIM
Pseudomonas putida 12969 plasmid p12969-2
DNA section
IS Length : 2620 bp

Ends


IR Length : 38/48

IRL : ACCCATTCCACGCGTATCCGACCACTGATTCCACGGTCATCCGGCCACTC
IRR : ACCTGTTCCACGGCCATCCGGCCGGGCAGTCGGAGCGCAGCGACGCAGGG

Insertion site


Left flankDirect repeatRight flankDR Length
AAGAGATCGTGATGGTTGGCATCCTGC7
GCACTGGATGGCTTCACCGCCTTACTGTTGA9

DNA sequence

TGCGGATTCCACGGTCATCTGGCCACCCATTCCATAAGCATCTGACCACCGATTCCACGGTGATCCGGCCACCCATTCCACGCGTATCCGACCACTGATT
CCACGGTCATCCGGCCACTCAACCGGGCAGGCAGTAACGCAGGATTTCTTCACTACCATCGACCTCTTTTTCGAAGCAGAGAGGTCGTCGTGGAGCGTTT
ATCCATGCGTAAGATTCGCGAAGTACTTCGTCTCAAGTTCGAGGTCGGGCTATCGGCTCGCCAGATTGCCGTCAGCGTGCAGATCGGTCGTGTCACTGTC
GGCGATTACCTCAACCGCTTTGCCGCCAGCGGTCTAGCCTGGCCCTGTTCGTTGTCCGATTCCGAGCTGGAGCAGCAACTGTTCCCACCGCCCCCTGCGG
TGCCCAGCGAGCAACGGCCATTGCCCGATTGGTCTTGGGTGCATGCCGAGCTACGCCGGCCGGGCGTGACCTTGGCGCTGCTCTGGCAGGAGTATCGCCT
GAGCCAGCCGAAAGGCTTTCAGTACAGCTGGTTCTGCGAGCACTACCGAGCCTGGCAGGGCAAGCTGGACGTGGTGATGCGCCAGGAGCATCGCGTCGGT
GAGAAGCTGTTTGTCGACTACGCCGGGCAGACGGTGCCGGTGATCGACCGCCACAGCGGCGAGATCCGCCAGGCGCAGGTGTTCGTCGCGGTGCTTGGCG
CGTCCAGCTACACCTTCGCCGAAGCCACTTGGTCGCAGCAGTTACCGGACTGGCTGGGCTCCCATGTCCGCTGCTTCGCCTTTCTCGGCGGCGTGCCGGA
AATCGTGGTGCCGGACAACCTGCGAAGCGCGGTGAGTAAGAGCCATCGCTACGAGCCGGACATCAACCCGAGCTACCGCGATCTGGCCGAGCACTATGGC
GTGGCGGTGGTGCCGGCGCGGGCGCGTAAGCCGCGCGACAAGGCTAAGGCTGAGGTCGGCGTGCAGGTGGTCGAGCGCTGGATCCTCGCCGCGCTGAGGA
ACCGCCAGTTCTTCTCCCTGGACGAACTCAACAGCGCCATCGCCTTATTGCTGGAGCGGCTCAACCGACGACCGTTTCGCAAGCTGCCGGGCTCCCGGCA
CTCGGCCTTCGAAGCCCTGGATCGTCCAGCGCTGAGCCCACTGCCGGAGCAGCCCTACGTCTATGCCGAGTGGAAGAAGGCGCGCGTGCATATCGACTAT
CACGTCGAGGTCGATGGGCATTACTACTCAGTGCCCTACCAGTTGGTGAAGAAACAGCTGGAAGTACGGCTGACGGCGCGCACCGTCGAGTGCTTCCACG
CCAACCAGCGGGTGGCCAGCCACCTGCGCTCGCCACACAAGGGCCGGCATAGCACACAGACCGAGCACATGCCCAAGAGCCATCGCGAGCATGCCGAGTG
GACGCCGCAACGGTTGATCCGCTGGGCCGAGCAGACCGGGCCGCACACCGCCGGCGTGATCAGCCACATCCTCGAACGGCGCATCCACCCAACCCAAGGC
TACCGGGCCTGCCTGGGCATCCTGCGCCTGGGCAAGACCCATGGCGAAGTGCGCTTGGAGCTGGCCTGCCGACGCGCCCTCAGCCTCGGCGCGTGCAGCT
ACAAGAGCCTCGAATCGATCCTGCGCCAGGGGCTGGAAAACCTGCCGCTGGCCCAGCAGCACCTGCCCCTGCTGCCGGACGACCACGCCAACCTGCGCGG
CCCCGGCTACTACCACTGAACACAAGGAATCCCACCATGCTGCCCCATCCGACCTTGGACAAGCTCCAGACCCTGCGCCTGACCGGCATGCTCAAGGCAC
TCGCCGAGCAACTGAAAACCCCCGACATCGACAGCCTGAGCTTCGTGGAACGCCTCGGCCTGTTGGTCGACCGCGAACTGACCGAGCGCGACGACAAGCG
CCTCAGCAGCCGCCTGCGTCAGGCCCGGCTCAAGCACAACGCCTGCCTCGAAGACATCGACTACCGCAGCCCGCGCGGACTCGACAAGGCGCTGATCCTC
CAGCTGAGCAGCGGTCAGTGGTTACGCGACGGACTCAACCTGATTATCAACGGCCCGACTGGCGTCGGTAAGACCTGGCTGGCCTGCGCCCTGGCCCACC
AGGCCTGCCGAGAGGGTTACAGCGTGCGTTACCTGCGCTTGCCGCGCCTGTTGGAAGAGTTGGGCCTAGCCCACGGCGACGGGCGCTTCGCCAAGCTGAT
GAGCGGCTATGCCAAGACCGACCTGCTGATCCTTGATGACTGGGGTCTGGCCCCGTTCAACGCGGAACAGCGCCGCGACATGCTGGAGCTACTGGACGAC
CGCTACGGCCAGCGCTCGACCATCGTGACCAGCCAAATGCCAGTGGACAACTGGCACGAACTGATCGGTGATCCGACTCTGGCCGACGCCATCCTCGACC
GCCTGGTGCACAACGCTTATCGGATCAACCTCAAGGGTGAGTCGATGCGCAAACAGACGAAGAAATTGACGACACCGGGCACCTCAGACTAACAATGCCC
CCCCTGCGTCGCTGCGCTCCGACTGCCCGGCCGGATGGCCGTGGAACAGGTGGCCAGATGGCCGTGGAATGCCTGGCCAGATGAGAGCGGACTGGGTGGC
CGGATGGCGTGGAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1515 bp504 aa2051719+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIREVLRLKFEVGLSARQIAVSVQIGRVTVGDYLNRFAASGLAWPCSLSDSELEQQLFPPPPAVPSEQRPLPDWSWVHAELRRPGVTLALLWQEYRLS
QPKGFQYSWFCEHYRAWQGKLDVVMRQEHRVGEKLFVDYAGQTVPVIDRHSGEIRQAQVFVAVLGASSYTFAEATWSQQLPDWLGSHVRCFAFLGGVPEI
VVPDNLRSAVSKSHRYEPDINPSYRDLAEHYGVAVVPARARKPRDKAKAEVGVQVVERWILAALRNRQFFSLDELNSAIALLLERLNRRPFRKLPGSRHS
AFEALDRPALSPLPEQPYVYAEWKKARVHIDYHVEVDGHYYSVPYQLVKKQLEVRLTARTVECFHANQRVASHLRSPHKGRHSTQTEHMPKSHREHAEWT
PQRLIRWAEQTGPHTAGVISHILERRIHPTQGYRACLGILRLGKTHGEVRLELACRRALSLGACSYKSLESILRQGLENLPLAQQHLPLLPDDHANLRGP
GYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
656 bp251 aa17372392+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPHPTLDKLQTLRLTGMLKALAEQLKTPDIDSLSFVERLGLLVDRELTERDDKRLSSRLRQARLKHNACLEDIDYRSPRGLDKALILQLSSGQWLRDGL
NLIINGPTGVGKTWLACALAHQACREGYSVRYLRLPRLLEELGLAHGDGRFAKLMSGYAKTDLLILDDWGLAPFNAEQRRDMLELLDDRYGQRSTIVTSQ
MPVDNWHELIGDPTLADAILDRLVHNAYRINLKGESMRKQTKKLTTPGTSD

 

Blast result :
Comments
References
1] Dongsheng Zhou (2015) Direct submission
2] Sun,F., Zhou,D., Wang,Q., Feng,J., Feng,W., Luo,W., Liu,Y., Qiu,X., Yin,Z. and Xia,P. (2015) J. Antimicrob. Chemother. In press