ISfinder Mirror Site

ISTer1

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008312	ND	Trichodesmium erythraeum	Trichodesmium erythraeum IMS101

DNA section

IS Length : 942 bp

Ends

IR Length : 21/23

IRL : GCAGTCGAAGCAAAGATTAGGACATAATGCTCGAAAAATTATCTAGGAAA
IRR : GCAATCGAAGCAAAGGTTAGGACGTTAACTTAACAGCATAATCAACTCTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGGTTTGTGT	TATA	AAAAACGATC	4

DNA sequence

GCAGTCGAAGCAAAGATTAGGACATAATGCTCGAAAAATTATCTAGGAAAGATTATGCCTTCTCCTTATAGCTATGACTTAAGACAAAAAGTTATTAAGG
CCATTGATGGTGGGATGAGTAAAACTCAAGTAAGTTCTATATTTAAGATTAGTCGTAATACCATCAATACTTGGTTACATAGAAGAAGAGAAACTGGGGA
TATAAAAGCAAAAACAGGTTATCAAAAAGGATATAATCCCAAAATACCAGACTTAGAACAATTCAGAAAATTTGCCCAGATTAATGGCAGTAAAACTCAA
AAAGAAATGGCTGATGAATGGCCAGATAAGGTAAGCGATCGCACTATAAGTAAAGCCTTAAAAAAAATTGGTTATACAAGAAAAAAAAACTTACGGTTAC
AGAGAAAGAGATGAAGACAAAAGAAAGAAATTTCTGGCCAAAATTCGCGATAAAAGGCCGGAACAGCTAGTGTATATAGATGAATCAGGTATGGATAATA
GAGAAGATTATGGTTATGGATGGAATGAATTGGGAAAAAGATATTACGATTTAAAGTCAGGAAAAAGAAGTCTAAGGGTAAGTATTGTAAGTGGTTTGTG
TGAAGGAAAATTAGTTGCTCCATTAACATTTGAAGGCTCTTGTAACCGTGTAGTTTTTGAAAAATGGTTGAGAGAAAAATTGTTGCCACAATTAAAAACA
GGACAATTAGTAATATTAGACAATGCAAGTTTTCATAAATCCCAAAAAGTTAGAGAATTAATAGAATCAGTGAATTGTGAGCTTGAATATTTACCACCAT
ATTCTCCTGACTTAAATGAAATAGAACATTATTGGTTTCCAATTAAAAATCGAGTCAGAAAGTCAGAAGGAACAATAGAAAATTTTCGCATTGAGAGTTG
ATTATGCTGTTAAGTTAACGTCCTAACCTTTGCTTCGATTGC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
360 bp	119 aa	55	414	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPSPYSYDLRQKVIKAIDGGMSKTQVSSIFKISRNTINTWLHRRRETGDIKAKTGYQKGYNPKIPDLEQFRKFAQINGSKTQKEMADEWPDKVSDRTISK
ALKKIGYTRKKNLRLQRKR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
540 bp	179 aa	362	901	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKLVIQEKKTYGYRERDEDKRKKFLAKIRDKRPEQLVYIDESGMDNREDYGYGWNELGKRYYDLKSGKRSLRVSIVSGLCEGKLVAPLTFEGSCNRVVFE
KWLREKLLPQLKTGQLVILDNASFHKSQKVRELIESVNCELEYLPPYSPDLNEIEHYWFPIKNRVRKSEGTIENFRIES

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
847 bp	281 aa	55	901	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPSPYSYDLRQKVIKAIDGGMSKTQVSSIFKISRNTINTWLHRRRETGDIKAKTGYQKGYNPKIPDLEQFRKFAQINGSKTQKEMADEWPDKVSDRTISK
ALKKLVIQEKKTYGYRERDEDKRKKFLAKIRDKRPEQLVYIDESGMDNREDYGYGWNELGKRYYDLKSGKRSLRVSIVSGLCEGKLVAPLTFEGSCNRVV
FEKWLREKLLPQLKTGQLVILDNASFHKSQKVRELIESVNCELEYLPPYSPDLNEIEHYWFPIKNRVRKSEGTIENFRIES

Blast result :

Comments

ISTer1 is 72% aa similar to ISAcma1. The third ORF is the putative ORFAB tranposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2015)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Munk,A.C., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E. and Richardson,P. (2006) Direct submission GenBank.