ISPsy33
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP011972 | ND | Pseudomonas syringae | Pseudomonas syringae pv. actinidiae ICMP 18884 |
DNA section
IS Length : 1429 bp
Ends
IR Length : 18
IRL : CAATGCTGTTCACTTAAGGATTGTTGACTAATCCTCGGCCAGCAGGAAAA
IRR : CAATGCTGTTCACTTAAGCGGTGCAACCTTGCGGTTAACCGGATACCGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGAAAAAGCC | CGCAGCGACGGCG | GGCTTTTTCT | 13 |
| CGAAAAAACC | CGCCAAAAGGCG | GGTTTTTCGG | 12 |
| CTGTACATGC | CGACCCTGACCC | TGATGTTTCT | 12 |
| GCCTAAGCGG | CGCAGCGACG | 0 | |
| TCATCGAGCG | CGCCAAGTGC | 0 | |
| GCCAAGTTCG | CGCTCATCGA | 0 | |
| CAAAAAAGCC | CCGATTACGG | GGCTTTCACG | 10 |
| TCAGATAGCC | CCGTGATTACGG | GGCTTTTTTT | 12 |
| AAAAAATCCC | CGAACCAGTCGG | GGATTTTTTA | 12 |
| AAAAACGGTC | CGCGTTGCGCG | GGCCGTTTAA | 11 |
| CCAAAAAGCC | CTGTCCTGTGACAG | GGCTTTTTTG | 14 |
| CAAAAAAACC | CCGTCACTGACG | GGGTTTTTTA | 12 |
| GTTATAAGCG | CGCTCGAAGC | 0 | |
| AAGAAAAGCC | CGCCAAGTGCG | GGCTTTTTGT | 11 |
| AAAAAAAGGC | CACCTGAAGGC | AGCCTTTAAA | 11 |
| TCAACATCGC | CAGCCACACCAG | GCGTTTCTCG | 12 |
| CTCGAAGCGG | GTAGTTATAA | 0 | |
| GAGAAAAGAC | CGGGCCTCACCG | GGCATGACGA | 12 |
| ACGAAAAAGC | CCCCGAGTCGGG | GGCTTTTTCG | 12 |
DNA sequence
CAATGCTGTTCACTTAAGGATTGTTGACTAATCCTCGGCCAGCAGGAAAATCCGGAATCTGATTATGGATTCCGGATTTTTTATGTCTGTCGCTCAAGAT
TTAGGCGCTGTTCTGGACTTTGCCGAGCAGCCGCTCTCACGCCTCGACGTATTCACCGATCATATTCCCCAAGAATGGATTAGCACTGCCGCCACTTTGG
CCGAAAAGGCCACGGTCCGACGTCGGCGCCTGCCCTCAGATATGGTGCTTTGGTTAGTCGTCGGCATGGCTCTTTTCAGAGGAGAGCCCATCGTTGAGGT
CGCGCGCCGACTCAATATCTGCGCCGACGGTCTAGCCAACGAGGTACTTTTGGCGAAGAGCGGCCTTTCACAGGCGCGTCAGCGCCTGGGCAATCAGCCT
GTGTCTTGGCTATTTCGACAGTGCGCCAGTACCTGGGGATACGAGCGCTACCCGCAGGATGACTGGCACGGACTTCAGGTTTTTGCCGTCGATGGTGCGC
TGTTTCGAACCCCGGAAACACCTCAGTTACGTGCGCACTTTGGCTCAGGCAACACCTCCTCCAATCGGCAGACGCCCTACCCGGTGTTGCGTCTTGTCGC
GCTGATGAATGCACGCTCCCACATCATTGCCAATGCTGCCATTAGCCCCTATCGCAAGGGTGAAATCCCACTGGCTAAGGACTTTGTGGAAAGCATACCC
AATCACTCGGTGACCTTACTGGACAAAGGGTTTTTCAGCGCTGATCTGTTGTTGAGTATCCAAAACGCTGAGCAAAACAGGCACTGGATGATCCCTGAAC
GCAAAGGAACGGTCAGGACGGAAGTCGAACATTATGGCGATGGCGATTATCTGGTGCAGATGAGGGTCTCGCCGCAGGCGCGTAAAAAGAATCCGGCATT
ACCTGAGTACTGGCAGGTAAGAGCTGTGACTTACCAAGTTGAAGGAAATGAGAAAACGGTCTTCACCTCGCTACCGGCTTCGAAATTTAGCGCAGAACAG
GTTGCGACGCTTTATCACGAGCGGTGGGAGATCGAGTTGGGTTTCAGAGATATCAAAAGCTCAATGCAAGACAATGCTCTGACGCTTCGCAGCAAGACCG
TGGACCTTGTTTACCAAGAGTTGTGGGGGCTTTTACTGGCGTATAACGTGGTGCGCCGAGAAGCCAGTCAGGCGGCTGTCGCTCACAAGCGGGCGCCCAG
CGAGATAAGTTTCAAGTTTGCCTGTCAGCACATTGCCAGTCATCTGGTGGTCATGGCCGGGGCCGTGTCGCCATCACATACGCCGAGGCGCCTGGAGGAA
CTGCGTGGCAGCATCGGTGTGCTCTTCATAACAAAACGCCCTAGGCCGGCGAGGCCCAGGGCGGTGAAGATGTCAAAAACCCGGTATCCGGTTAACCGCA
AGGTTGCACCGCTTAAGTGAACAGCATTG
TTAGGCGCTGTTCTGGACTTTGCCGAGCAGCCGCTCTCACGCCTCGACGTATTCACCGATCATATTCCCCAAGAATGGATTAGCACTGCCGCCACTTTGG
CCGAAAAGGCCACGGTCCGACGTCGGCGCCTGCCCTCAGATATGGTGCTTTGGTTAGTCGTCGGCATGGCTCTTTTCAGAGGAGAGCCCATCGTTGAGGT
CGCGCGCCGACTCAATATCTGCGCCGACGGTCTAGCCAACGAGGTACTTTTGGCGAAGAGCGGCCTTTCACAGGCGCGTCAGCGCCTGGGCAATCAGCCT
GTGTCTTGGCTATTTCGACAGTGCGCCAGTACCTGGGGATACGAGCGCTACCCGCAGGATGACTGGCACGGACTTCAGGTTTTTGCCGTCGATGGTGCGC
TGTTTCGAACCCCGGAAACACCTCAGTTACGTGCGCACTTTGGCTCAGGCAACACCTCCTCCAATCGGCAGACGCCCTACCCGGTGTTGCGTCTTGTCGC
GCTGATGAATGCACGCTCCCACATCATTGCCAATGCTGCCATTAGCCCCTATCGCAAGGGTGAAATCCCACTGGCTAAGGACTTTGTGGAAAGCATACCC
AATCACTCGGTGACCTTACTGGACAAAGGGTTTTTCAGCGCTGATCTGTTGTTGAGTATCCAAAACGCTGAGCAAAACAGGCACTGGATGATCCCTGAAC
GCAAAGGAACGGTCAGGACGGAAGTCGAACATTATGGCGATGGCGATTATCTGGTGCAGATGAGGGTCTCGCCGCAGGCGCGTAAAAAGAATCCGGCATT
ACCTGAGTACTGGCAGGTAAGAGCTGTGACTTACCAAGTTGAAGGAAATGAGAAAACGGTCTTCACCTCGCTACCGGCTTCGAAATTTAGCGCAGAACAG
GTTGCGACGCTTTATCACGAGCGGTGGGAGATCGAGTTGGGTTTCAGAGATATCAAAAGCTCAATGCAAGACAATGCTCTGACGCTTCGCAGCAAGACCG
TGGACCTTGTTTACCAAGAGTTGTGGGGGCTTTTACTGGCGTATAACGTGGTGCGCCGAGAAGCCAGTCAGGCGGCTGTCGCTCACAAGCGGGCGCCCAG
CGAGATAAGTTTCAAGTTTGCCTGTCAGCACATTGCCAGTCATCTGGTGGTCATGGCCGGGGCCGTGTCGCCATCACATACGCCGAGGCGCCTGGAGGAA
CTGCGTGGCAGCATCGGTGTGCTCTTCATAACAAAACGCCCTAGGCCGGCGAGGCCCAGGGCGGTGAAGATGTCAAAAACCCGGTATCCGGTTAACCGCA
AGGTTGCACCGCTTAAGTGAACAGCATTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1338 bp | 451 aa | 83 | 1420 | + | No |
Chemistry : DDE
ORF sequence :
MDSGFFMSVAQDLGAVLDFAEQPLSRLDVFTDHIPQEWISTAATLAEKATVRRRRLPSDMVLWLVVGMALFRGEPIVEVARRLNICADGLANEVLLAKSG
LSQARQRLGNQPVSWLFRQCASTWGYERYPQDDWHGLQVFAVDGALFRTPETPQLRAHFGSGNTSSNRQTPYPVLRLVALMNARSHIIANAAISPYRKGE
IPLAKDFVESIPNHSVTLLDKGFFSADLLLSIQNAEQNRHWMIPERKGTVRTEVEHYGDGDYLVQMRVSPQARKKNPALPEYWQVRAVTYQVEGNEKTVF
TSLPASKFSAEQVATLYHERWEIELGFRDIKSSMQDNALTLRSKTVDLVYQELWGLLLAYNVVRREASQAAVAHKRAPSEISFKFACQHIASHLVVMAGA
VSPSHTPRRLEELRGSIGVLFITKRPRPARPRAVKMSKTRYPVNRKVAPLK
LSQARQRLGNQPVSWLFRQCASTWGYERYPQDDWHGLQVFAVDGALFRTPETPQLRAHFGSGNTSSNRQTPYPVLRLVALMNARSHIIANAAISPYRKGE
IPLAKDFVESIPNHSVTLLDKGFFSADLLLSIQNAEQNRHWMIPERKGTVRTEVEHYGDGDYLVQMRVSPQARKKNPALPEYWQVRAVTYQVEGNEKTVF
TSLPASKFSAEQVATLYHERWEIELGFRDIKSSMQDNALTLRSKTVDLVYQELWGLLLAYNVVRREASQAAVAHKRAPSEISFKFACQHIASHLVVMAGA
VSPSHTPRRLEELRGSIGVLFITKRPRPARPRAVKMSKTRYPVNRKVAPLK
Blast result :
Comments
ISPsy33 is 86% aa similar to ISAeme15.
References
1] Margareth Butler (2015) Direct submission
2] McCann,H.C., Rikkerink,E.H., Bertels,F., Fiers,M., Lu,A., Rees-George,J., Andersen,M.T., Gleave,A.P., Haubold,B., Wohlers,M.W., Guttman,D.S., Wang,P.W., Straub,C., Vanneste,J.L., Rainey,P.B. and Templeton,M.D. (2013) PLoS Pathog. 9 (7), E1003503.
2] McCann,H.C., Rikkerink,E.H., Bertels,F., Fiers,M., Lu,A., Rees-George,J., Andersen,M.T., Gleave,A.P., Haubold,B., Wohlers,M.W., Guttman,D.S., Wang,P.W., Straub,C., Vanneste,J.L., Rainey,P.B. and Templeton,M.D. (2013) PLoS Pathog. 9 (7), E1003503.