ISAeme21

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP007567 ND Aeromonas media
Aeromonas media WS
DNA section
IS Length : 1038 bp

Ends


IR Length : 23/31

IRL : GCTAGCTGTGATCTCTCTGTAGGTTGTTCACTCGGGGCATTCCCGGAGAA
IRR : GCTAGCTGTGTAAACCCACCAGGTTGTTCACAGACTGAAGACGACTGATG

Insertion site


Left flankDirect repeatRight flankDR Length
GAGTGGGCGGGCGCCCGACA0
TCGCACCTTCTCTTACTCTG0
ATGATCGGGCCTGGGATTCT0
AAGAAGCAGACAGCAGTGTA0

DNA sequence

GCTAGCTGTGATCTCTCTGTAGGTTGTTCACTCGGGGCATTCCCGGAGAATTGGAGGCGCCAACCAAACCAATCACCCGAGGTGCCCCAGTGAACAACCA
TAAAAATGCCCGATTAACCGTCTTCGGTCGACAGCTTCTTGTCCAGCGTATCCTTGAGCAGGGCCTGCGCGTTGAAGAGGCCGCTCACGCTGCCGGCGTC
AGTACCCGCACCGCTTACAAGTGGCTCACTCGATATCGCCAGGAGGGGCCTGATGGCTTGTACAATCGCTCTTCTCAACCTGCATCCTGCCCCCATGCCA
TTGATGACCTGCGCCAACAGAGGATTATCGAACTGCGCAAACAGCGACAGACCTATCGGCACATCAGCCAATCTCTGGGCATAGGGCATAGTACTGTCGC
TCGTATCCTGCAACGAATGGGACTGAATCGACTGGCTAATCTGGAACCTGCCCCTCCAGTACAACGCTATGAACATGAGAAACCGGGTGACTTGCTGCAT
TTGGACATCAAAAGGTTGGGGCGTTTCCATCGTCCCGGCCATCGGGTGACTGGTGACCGCCGCCAGGACTCTCCGGGGGCAGGATGGGAGTATGCGCATG
TGGCGGTTGATGACCACTCCCGTCTGGCATTCAGCGCCCTGTATCCTGATGAAACGGGACGCAGTGCCTGCCTGGCACTATTACAGGCACTTCGCTACTA
CGCCGGGCTCGGTATTCGTTTCAGTCGGCTACTCACTGACAATGGCGCCTGTTATCGGTCGAAACGCTTCCAGAAGCTGTGTCGTCGCTTGGGACTCAAG
CACCTGCGCACCAAACCTTACACACCCCAAACGAATGGCAAAGCAGAGCGCTTCATTCAGACAGCGTTACGTGAATGGGCTTATGCACGGAGCTATGACT
CATCAGTGCAACGGGCAGAACACTTACAACCGTGGCTGCATGAGTACAACTGGCATCGTCCTCATGCAAGTCTGGGCTACCAGCCCCCCATCAGTCGTCT
TCAGTCTGTGAACAACCTGGTGGGTTTACACAGCTAGC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
948 bp315 aa901037+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNNHKNARLTVFGRQLLVQRILEQGLRVEEAAHAAGVSTRTAYKWLTRYRQEGPDGLYNRSSQPASCPHAIDDLRQQRIIELRKQRQTYRHISQSLGIGH
STVARILQRMGLNRLANLEPAPPVQRYEHEKPGDLLHLDIKRLGRFHRPGHRVTGDRRQDSPGAGWEYAHVAVDDHSRLAFSALYPDETGRSACLALLQA
LRYYAGLGIRFSRLLTDNGACYRSKRFQKLCRRLGLKHLRTKPYTPQTNGKAERFIQTALREWAYARSYDSSVQRAEHLQPWLHEYNWHRPHASLGYQPP
ISRLQSVNNLVGLHS

 

Blast result :
Comments
ISAeme21 is 77% aa similar to ISStma3.
References
1] Pfeiffer F. (2015) Direct submission
2] Chai B., Wang H. and Chen X. (2012) J. Bacteriol. 194 (23), 6693-6694