ISHel2

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AOLK01000005 ND Haloferax elongans
Haloferax elongans ATCC BAA-1513
DNA section
IS Length : 1975 bp

Ends


Left end : AGAGGAAACAAGGCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGCCGACACTTAGACGTGTTCTGTCCGCTCGATGTACCGCTCAATCGTTCCCGTTG II struct. : No
Right end : ACCCACCCGACCGCCCGAGGCTATCAGGCCGATGGTCAGGTGGGAGTATCTGATTAAACCACGGGAAGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CAATATGGACGTCTACGTGCGGTTTCAC

DNA sequence

AGAGGAAACAAGGCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAGCCGACACTTAGACGTGTTCTGTCCGCTCGATGTACCGCTCAATCGTTCCCGTTG
AGACTGTTCCTGCCGTTCCAATGTAGTACGACCGTTCCCAGAATCCACCACCCCACAAATACTCCTCTAAGAACGACTCGTGCTGTTCCCACATCTCCCG
CGCCGTGATGCTCTTGACAGTCCGTGCAATCTCGCTCGGCGCGTGTTTCGGGTGGGCGGAGAGGAACAAATGTACGTGGTCGGGAGAGATGTGGAGCGAC
AGTATCTCGTAATCATACTCGTCACACACGTCGCGGAAACTCGTTTCCAACGAATCCTCGATGGGTTCGAGAATCGCGTGACGGTACTTCGGACACCACA
CGAAGTGGTAGTTGACGTTGTACACTGTGTGATTCGACTGCTTCTCACCCATATCGAACCCACTCCACCAACACAGTTAAGTATATACAAAGGATATACG
AAAGTATGGCGAATCGCTTTGAAATCGACGGCGAGGAAGTTCTCGACGGTGAAGTCAAACCGTTCGGGAATAGTGCCCACGTCACCGTCCCTAAACGCTG
GCGTGGGGCCGACGTGAAAGTCGTCCGAACCTCAGAACCCACCGAACAAGACGAAGAATGACCGACTCACAGGCTCTCGTCAAGACTCTGGACTTCCAAC
TCAACATCCAGAGTGACAACGAGAGCCTGTTGTACGACGCCACGCTTGAAGCCCGCTCGGTGTACAACGAAACCACCCGTCTCGCCAAAGAAGGTGTGGA
CTGGAACGCGATTCCCGACCGCGTAGCCGACGACGCCAACCTCGTGAAAAACACGACTCAGCGCGTTGTCGCCAAGGCTCTCGGAGCGATGGAGAACTAC
TACGAGTACGACGACTTCGGACTCCCCAGTCACACCAAGGGTGGTTCGTACCCGCTTCGTGCGAACTACGAGGAGGGGTACAACCTGTCGCTCACCGACG
ATGGCGACGTGGCGTTCCGAATCAGCGCGAAACCGTACAAGCACGTCAAGGGCGTCCTCAAAGGGAGTGACGCTCACCTCGACATTCTCAAGATCGCGCT
CGAAAACGACGAGTGGAAGATTGGGACGGCGGAAGCCCTGTTCCACAACGACAACGCCGAACTACACGTTAACGTTACCAACACCGAGCAGACCGTGCGA
GACAAGCACGACTCGCGGACGGTCGTCGGCGTGGATGTGAACGAGGACAACGTGGCTCTCACCGCTCTCTCAGAGGATGGTGTCGAAGACACGTTGGTTA
TCGACTTTCCCGAAATCAAGTTCGAGCGCCACCGCTACTTCACGATGCGAAAGCGTGTTCAGAACGCGGGGAAAGACAGCATCCACGACACACTCGAAGG
GCGTGAAGAACGGTTTGTCCGCGACCGACTCCACAAGGTAAGCCGTCACATCGTGGAGTGGAGCCGTCAGTTCGAGAAGCCGTGCATCGTCTTTGAAGAC
CTCAAAGAGATGCGCGACAGTATCGACTACGGCACGCGGATGAACCGACGGTTGCACCACCTTCCCTTCCGCGCCCTCCAGTTCTATACATCGTACAAGG
CGTCGTTCGAGGGTATCCCGACTGCGTGGATTAACCCCGAATACACTAGCCAGCGGTGTCCGATGTGCGGACACGCCGAGCGTGCGAACCGCAACAAGAA
GCGGTTCAAGTGCAAGTCCTGTGGGCATCAAGACCACAGCGACCGTGGTGCAAGCGTCAACATCGCCGTGAAAGGCGTGAAGAAACTCGATTGGAATGTG
CCTGCTCTCAACAGCCTTCCCGTTGTTCGGAAGGTGCGACGGCAGGCATCGGGGCTTGTGGACAGCCCGACCGTGACCCACCCGACCGCCCGAGGCTATC
AGGCCGATGGTCAGGTGGGAGTATCTGATTAAACCACGGGAAGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa45254-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGEKQSNHTVYNVNYHFVWCPKYRHAILEPIEDSLETSFRDVCDEYDYEILSLHISPDHVHLFLSAHPKHAPSEIARTVKSITAREMWEQHESFLEEYLW
GGGFWERSYYIGTAGTVSTGTIERYIERTEHV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1275 bp424 aa6581932+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTDSQALVKTLDFQLNIQSDNESLLYDATLEARSVYNETTRLAKEGVDWNAIPDRVADDANLVKNTTQRVVAKALGAMENYYEYDDFGLPSHTKGGSYPL
RANYEEGYNLSLTDDGDVAFRISAKPYKHVKGVLKGSDAHLDILKIALENDEWKIGTAEALFHNDNAELHVNVTNTEQTVRDKHDSRTVVGVDVNEDNVA
LTALSEDGVEDTLVIDFPEIKFERHRYFTMRKRVQNAGKDSIHDTLEGREERFVRDRLHKVSRHIVEWSRQFEKPCIVFEDLKEMRDSIDYGTRMNRRLH
HLPFRALQFYTSYKASFEGIPTAWINPEYTSQRCPMCGHAERANRNKKRFKCKSCGHQDHSDRGASVNIAVKGVKKLDWNVPALNSLPVVRKVRRQASGL
VDSPTVTHPTARGYQADGQVGVSD

 

Blast result :
Comments
ISHel2 is 93% (TnpA : the transposase) aa similar to ISH36 and 96% (TnpB) aa similar to ISHma7.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.