ISHas1

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ARQA01000287 ND Halonotius sp.
Halonotius sp. J07HN6
DNA section
IS Length : 1955 bp

Ends


Left end : AGATAAACAAGGCGAGTGCCTCGGGGGTTGACCCCGAGGGTGAAGCCGACACTCAGACGTGTTCTGTTCGTTCGATATACTGTTCAATCGTCTCGTTGGA II struct. : No
Right end : GTGACCCACGACACCGTTCAAGGCTATCAGACCGATGGTGTCGCGGGCGTGTCCGACTAATCCACGGGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CGAGGACGAGTTCTACATTTCAGTGTTT

DNA sequence

AGATAAACAAGGCGAGTGCCTCGGGGGTTGACCCCGAGGGTGAAGCCGACACTCAGACGTGTTCTGTTCGTTCGATATACTGTTCAATCGTCTCGTTGGA
TACCTGCCCCGCCGTTCCGATGTAGTACGACTGCTCCCAGAACCCACTCCCCCACAGATATTCTTTCAAGAACGATTCGTGCTGTTCCCACATCTCCCGA
GCCGTGATGCTCTTGACAGTCCGTGCGATCTCGCTCGGCGCGTGCTTCGGGAGGCCTGACAGAAACAGGTGTACGTCGTCGGGTGATATATGGAGTGATA
GAATCTCGTAGCCATACTCCTCACACACATCTCGGAAACTCGCTTCCAGCGAATCGTCGATTGGGTTGAGAATCGCGTGGCGATACTTCGGACACCACAC
GAAGTGGTAGTTGACGTTGTATACCGTATGGTTCGACCGTTTCTCACCCATACAGACAGTACGTGGGCAATAGTTAAGTATTTCAGTATCTAAGTAAGTG
TGTGAGATGACGAAACGACTGTCCGTAGACTTCGAGGACGATGTGTACAAGGAGTTCTCGAAGAAGTGCATCGACGTTGATGAAACCAAGTCCGACGTAG
TGCGCGGACTCGTCACCGACTGGCTCAACGAAATAGAAGAATGACTGACGCACAGGTTCTCGTCAAGACGCTGGACTTCCAACTCGACATCCAGAGTGGC
AATGAGAACCTGCTGCACGACGCCTGTCTCGAAGCACGGTCGGTGTACAATGAAACCATCCGCCTCGCCAAACAGGGCGTGGACTGGGATGCAATTCCTG
ACCTCGTGGCCGACGACGCCGATCTCGTGAAGAACACGACGCAGCGCGTCGTTGCGAAGACACTCGGCGCGATGGAGAACTACTACGAGTACGACGACTT
CGGCCAGCCGAGCCACACCAAGGACGGCGCGTACCCGCTTCGAGCCAACTACGAAGAAGGGTACAACCTGTCGCTCACCGACGACGGCGACGTGGCGTTC
CGCATCAGCGCGAAACCGTACAAGCACGTCAAAGGTGTCCTCGACGGTGACGACGCCCACCTCGACATTCTCAAGACCGCACTCGAAAGCGACGAGTGGA
AGATTGGAACGGCAGAAGCTCTGTTTCACAACGACAACGCCGAGCTGCACGTCAACGTCATCAACACCGAGCAGACCGTCCGCGACAAGCAGGACTCACA
GACTGTCGTTGGTGTGGACGTGAATGAGGACAACGTGGCTCTGACCGCGCTCTCCGAGAACGGTGTCGAAGATACGTTGGTCATCGACTTCCCCGAAATC
AAGTTCGAGCGTCACCGCTATTTCACGATGCGGAAGCGCGTGCAGAACGCGGGGAAAGAGAGCATCCACGACACGCTGGGAGGGCGTGAGGAACGGTTCG
TCCGCGACCGACTCCACAAGGTATCTCGGCACATCGTGGAGTGGAGCCGTCAGTTCGAGAGGCCGTGCATCGTCTTTGAAGACCTCAAAGAGATGCGCGA
CAGTATCGACTACGGCACACGGATGAACCGACGCTTGCACCACCTGCCGTTCCGCGCGCTTCAGTTTTATACGTCGTACAAGGCGTCGTTCGAGGGTATC
CCGACGGGCTGGATTGACCCGTACAAGACAAGTCAACGGTGTCCGATGTGCGGGCACGCCGAGCGAGCGAACCGCAACAAAAAGCGGTTCAACTGTCGCT
CGTGTTCTCATCAAGACCACAGCGACCGTGGTGCAAGCGTCAATATCGCCGTGAAAGGTATCAAGAAGCTTGATTGGAATGTGCCTGCTCTCAACAGCCT
CCCCCAAGTTCGGAAGGTGCGACGGCAGGCATCGGGGGCCGTGGACGCCCCGACCGTGACCCACGACACCGTTCAAGGCTATCAGACCGATGGTGTCGCG
GGCGTGTCCGACTAATCCACGGGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa45153-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGEKRSNHTVYNVNYHFVWCPKYRHAILNPIDDSLEASFRDVCEEYGYEILSLHISPDDVHLFLSGLPKHAPSEIARTVKSITAREMWEQHESFLKEYLW
GSGFWEQSYYIGTAGQVSNETIEQYIERTEHV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1275 bp424 aa6411915+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTDAQVLVKTLDFQLDIQSGNENLLHDACLEARSVYNETIRLAKQGVDWDAIPDLVADDADLVKNTTQRVVAKTLGAMENYYEYDDFGQPSHTKDGAYPL
RANYEEGYNLSLTDDGDVAFRISAKPYKHVKGVLDGDDAHLDILKTALESDEWKIGTAEALFHNDNAELHVNVINTEQTVRDKQDSQTVVGVDVNEDNVA
LTALSENGVEDTLVIDFPEIKFERHRYFTMRKRVQNAGKESIHDTLGGREERFVRDRLHKVSRHIVEWSRQFERPCIVFEDLKEMRDSIDYGTRMNRRLH
HLPFRALQFYTSYKASFEGIPTGWIDPYKTSQRCPMCGHAERANRNKKRFNCRSCSHQDHSDRGASVNIAVKGIKKLDWNVPALNSLPQVRKVRRQASGA
VDAPTVTHDTVQGYQTDGVAGVSD

 

Blast result :
Comments
ISHas1 is 92% (TnpA : the transposase) aa similar to ISH36 and 94% aa (TnpB) similar to ISHma7.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Podell,S., Ugalde,J.A., Narasingarao,P., Banfield,J.F., Heidelberg,K.B. and Allen,E.E.(2013) PLoS ONE 8 (4), E61692