ISHaca1

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AOLS01000016 ND Haloarcula californiae
Haloarcula californiae ATCC 33799
DNA section
IS Length : 1964 bp

Ends


Left end : CAGGCGGAACAGGCCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAAGCCGTAAGCTCGGCTAAACGTGTTCCGTTCGCTCAATGTACTGTTCAATCGTA II struct. : No
Right end : GTGGCCTCTGATACCGTTCGAGGCGACCAGACCAATGGTATCCGAGGCGTGTCCGACTAACCCACGGGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GGTCCTACGAAATCTACATCGAGCGTAT

DNA sequence

CAGGCGGAACAGGCCGAGTGCCTCGGGGCTTGACCCCGAGGGTGAAAGCCGTAAGCTCGGCTAAACGTGTTCCGTTCGCTCAATGTACTGTTCAATCGTA
TCGGTCGAAACGTCCCCGGCCGTCCCGACGTAGTACGATTCTTCCCAGAAACCTCCACCCCATAGATATTCTTGTAACAGGAATTCGTGTTGTTGCCACA
TCTCTCGTGCTGTAATACTCTTGACTGTCCGTACAATCTTGCTCGGAGCGTGTTTGGGATGTGTCGACAGGAACAGGTGTACGTGGTCGGGTGAGGTGTG
CAACGACAGTATCTCATAGTTGTACTTGTCGCACACCTCTCGAAAACTCGATTCTAGTGAATCTTCTATCACGCCCAGTATCGGGTGTCGATACTTTGGA
CACCACACAAAGTGGTAGTTGACGTTGTATACCGTGTGGTTTGAGCGTTTCTCGCCCATACAGACAGTACGTGGACAATTTTTAAGTATCTCAGTAAATA
AGCAGTGTCTGTGAAATGACTAAACGACTTACCGTGGACTTGGAGGACGAGTTATACAAGGAGTTCTCGAAGAAATGCATTGACGCTGAGAAAACCAAGT
CCGAAGTCGTGCGTGGGCTCGTCCAAGACTGGGTAAACGACCAAGAATGACGCAGACACAGGCTCTCACCAAGACACTCGTGTTCCCGCTTGACGTGCAG
AGTGGCAACGAGAGCCTGCTTCACGACGCCCGCTTGGAGTGTCGCCGCGTGTTCAATGAAGTGCTACGCCTCAACTACGACGGGTGGGACTGGGACGAGA
TAGAAGATGTTGTCGAGCAGAACGCAGACCTCGTGCAAAACACCGCACAGCGCATCATCAACAAAGCCTTCGACGCACTCAACAACTACTACGACTACGA
CGACTGGGGACGCCCGTGGTACAAGCACGAAACGTTCCCGTTGCGGATGAACTACGGCGAAGGCTATAACCTCTTTCTCGGAGACGAAACGGTGCGGTTC
CGCATCAGTGCGAAACCGTACAATCACGTCAAAGGCGAACTGTGCGGCACACAAGACCAGTTCGACCTGCTCACACAGGCGATGGAAGATGATGACTGGC
ACATTGGTACCGCCGAGGCGCTTGCCCGAAACGAACGCGAAGAATTGCACGTCACCGTCACGAACGAAACCGCCGAAGTTGGTGCAAAAACAGCGGCAGA
AACAGTCATCGGCGTCGATATTAACGAAGACTGTGTTGCGCTAGCGGCACTGACCGAGAGCGGTATCGAAGATTCAATCGTTATCGACTACCCAGAAATC
AAAGAAGAACGACATCGGTACTTCACGATGCGGAAGCGGATGCAGGAAGCCAGACAGACCGCGTTCAACGACGTGTTCCGTGACAAGGAACAACGGTTCG
TTCACGACCAGCTACACACGGTGTCACGGCGCGTAGTCGAGTGGATGCAACGGTTCGACAGTCCTGTGATTGTCTTCGAAGACCTCGAAGACATGCGAGA
CGACATTGAATACGGGACTCGAATGAACCGCCGTCTGCACTCGATTCCGTTCGCCAAACTCCGTGATTTCATCACGTACAAGGCGGCGTGGCAAGGGATT
CCGTCAGATGATGTTGACCCGGAGTACACCAGCCAGCAATGCCCACTGTGTGGACACACAGAACGGGCGAACCGCCACAAGAAACGGTTCAAGTGCTGTG
AGTGCGAGCATCAAGACCACGCCGACCGTGGCGCTGGTGTGAGTGTCGCGCAGAAATGGCTACGAAAGCAAGACAATAGAGATGTGCCTGCTCTCAACAC
ACTCCCGCAAGTGCGGAAGTGGGAGTTGCGACGGCAGGCATCGGGGCCTGTGGACGGCCCGACCGTGGCCTCTGATACCGTTCGAGGCGACCAGACCAAT
GGTATCCGAGGCGTGTCCGACTAACCCACGGGAAGCCTCGGGGCTTGACCCCGAGGCGGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
399 bp132 aa45961-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGEKRSNHTVYNVNYHFVWCPKYRHPILGVIEDSLESSFREVCDKYNYEILSLHTSPDHVHLFLSTHPKHAPSKIVRTVKSITAREMWQQHEFLLQEYLW
GGGFWEESYYVGTAGDVSTDTIEQYIERTEHV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1278 bp425 aa6471924+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTQTQALTKTLVFPLDVQSGNESLLHDARLECRRVFNEVLRLNYDGWDWDEIEDVVEQNADLVQNTAQRIINKAFDALNNYYDYDDWGRPWYKHETFPLR
MNYGEGYNLFLGDETVRFRISAKPYNHVKGELCGTQDQFDLLTQAMEDDDWHIGTAEALARNEREELHVTVTNETAEVGAKTAAETVIGVDINEDCVALA
ALTESGIEDSIVIDYPEIKEERHRYFTMRKRMQEARQTAFNDVFRDKEQRFVHDQLHTVSRRVVEWMQRFDSPVIVFEDLEDMRDDIEYGTRMNRRLHSI
PFAKLRDFITYKAAWQGIPSDDVDPEYTSQQCPLCGHTERANRHKKRFKCCECEHQDHADRGAGVSVAQKWLRKQDNRDVPALNTLPQVRKWELRRQASG
PVDGPTVASDTVRGDQTNGIRGVSD

 

Blast result :
Comments
ISHaca1 is 96% (TnpA : the transposase) aa similar to ISHti1 and 95% (TnpB) aa similar to ISHwa17.
References
1] Friedhelm Pfeiffer (2015) Direct submission.
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.