ISfinder Mirror Site

ISNch1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AOIN01000043	ND	Natrialba chahannaoensis	Natrialba chahannaoensis JCM 10990

DNA section

IS Length : 1636 bp

Ends

Left end : CTGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAGGGAGCGAGTAGGGAGGGGATACAGCGTCCCCAGAAATCTTCGATTTCTGGTGTGCG II struct. : Yes
Right end : CCCTGTGGATACGTCCGTATCTGCAAAGCGCGTCGTTGAATCAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TGTCTCTTCGAT	TTAC	GTGTGAACTCGA	tcac

DNA sequence

CTGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAGGGAGCGAGTAGGGAGGGGATACAGCGTCCCCAGAAATCTTCGATTTCTGGTGTGCG
AACGAGACGCGGAGCGTCTCGTCAACGCCGCACGGTTCTCAAACACGTTCGTCGCTCGGCCTACAGTCCCACGCAATCGCAACTGTTTAGTATGCAGTTT
GCATAACTTATGGTGTGGCAACGCGCAAGACCATCTCTATCCGCGACGACCAAGAGGAGTGGATTCAGGAGAACCACCTGAGTCTGTCCTCGTTCGTCCA
AGAGAAACTTGACGAACACATCGAAGAACGCTCGTAGATGAACTACAACTACAGGTATCGACTCCGACCGTCCGACGCTCTCGAAGAACAGTTAGCGTGG
ACTGTCAATACCTGTAGACAGGTCTACAACCACTTCCTCCACCGACTCAACCGAACCGACGATACCTCGGCATACAGCGAACAGAAACTCCTGCCGAGCC
TCAAGAAGTGGTGGAACGACCTGAAACACGTTCACTCGAAAGTGCTTCAGAAAGTCGTTCAGCGGTTGTACGACAACCTCTCGACGCTTCGCGGTCGCAA
AGACAACGGCTACCGCGTCGGCACGCTCAACTGGAAAGCACCGGGCGAGTACCGCAGTTTCACCTACAGTCAATCCGGCTTCAAGCTCAAGAACACGAGC
GGTCGGACCCGATTGTGGCTCTCGAAACTCGGAGAAATCCCACTCACCTTCCACCGTGACCTCCCCGACGACGCCGAAATCAAGACCGTCACGGTCAAAC
AGGAACCGACCGGGAAGTGGTACGCCATCCTCGGTGTCGAAACCCCTGACGACCCGCCGGAGAAACCGGAGAATCCCGAGCGGTACGTCGGCATCGACGT
GGGGATTCTCAAGTGCGCCCACGACACCGACGGAACCGCCGTCGAATCGCTTGACCTGTCCGACGAACGCGAACGGTTGGAACGCGCACAGCGTGACCTC
TCGCGGAAACAGCACGGGTCTGAGAATTGGGAGAAACAGCGTCGAGTCGTGGCCGAGCGTCACGCCGAGTTGAAGACCAAGCGCCGTGACTTCCTCCACA
AGCTCTCGAACTACTACGCCACCGAGTACGACTTTGTAGCGGTCGAGGACTTGGACGCGAAGGGCTTGGTCGAACTACCGGGCAACTCTCGGAACCGCGC
AGGGGCGGCGTGGGGGACGTTCCTACGGATGCTTGAGTACAAGTGTGAGCGGGAAGGAACGCACTTCGTCGCGGTGAATCCGCGTGGGACGACCAAAGAG
TGTGCGTCCTGTGGTGTTTCGACTGAGAAACCGCTGTGGGTGCGCGAACACTCCTGTCCGGCGTGTGGGTTTGAAGCGGATAGAGACGCGAACGCGGCAT
GGAATATTCTTTCTCGCGGTATCAAGAAGCGGTTAGGAGCGGGACGCTCCGAATCAACGTCCTCAGAATCGCACAGCGATTCTGATGGGCTGCGAAAATC
GCTCAGCGATTTTCGAACGCCTGTAGAGACTGCGCTCCCTGTGGATACGTCCGTATCTGCAAAGCGCGTCGTTGAATCAGGAAGCCCCACCCTCAAGCGC
GAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
123 bp	40 aa	215	337	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MATRKTISIRDDQEEWIQENHLSLSSFVQEKLDEHIEERS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1284 bp	427 aa	338	1621	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLRPSDALEEQLAWTVNTCRQVYNHFLHRLNRTDDTSAYSEQKLLPSLKKWWNDLKHVHSKVLQKVVQRLYDNLSTLRGRKDNGYRVGTLNWK
APGEYRSFTYSQSGFKLKNTSGRTRLWLSKLGEIPLTFHRDLPDDAEIKTVTVKQEPTGKWYAILGVETPDDPPEKPENPERYVGIDVGILKCAHDTDGT
AVESLDLSDERERLERAQRDLSRKQHGSENWEKQRRVVAERHAELKTKRRDFLHKLSNYYATEYDFVAVEDLDAKGLVELPGNSRNRAGAAWGTFLRMLE
YKCEREGTHFVAVNPRGTTKECASCGVSTEKPLWVREHSCPACGFEADRDANAAWNILSRGIKKRLGAGRSESTSSESHSDSDGLRKSLSDFRTPVETAL
PVDTSVSAKRVVESGSPTLKREPSGER

Blast result :

Comments

ISNch1 is 95% (TnpB) aa similar to ISNma17. The first ORf is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.