ISfinder Mirror Site

ISNje1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AOIA01000046	ND	Natronococcus jeotgali	Natronococcus jeotgali DSM 18795

DNA section

IS Length : 1597 bp

Ends

Left end : TGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAAGGAGCAAGTAGGGAGGGGATACAGCGCCGCACAGTTTTCATACACGTTCGACGCTCG II struct. : Yes
Right end : CCCTGTGGATACGTCCGTATCTGCAAAGCGCGTCGTTGAAACAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TGCCCGCTGTTC	TTAC	atttaacaag	tcac

DNA sequence

TGAATTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAAGGAGCAAGTAGGGAGGGGATACAGCGCCGCACAGTTTTCATACACGTTCGACGCTCG
GTCCACAGGCCCCCCGCCACCTTTATGTTAAAACGAGGTATAAACACAGGTGATGGATAGACATGAAATCGAAGGCCACGAAGTCATCGACGGGACGGCC
AAAGCCACCGGCAACGGCGCTCACGTCCTCGTCCCGAAACGGTGGCGTGGCGCAGACGTGAAAGTCATCCGAACCACCGAACCCGACGAGTAGCACCTCC
GATGAACTACAACTACAGGTATCGACTTCGACCGTCCGACGCTCTCGAACAGCAGTTAGCGTGGACTGTCGATACCTGTAGGCAGGTCTACAACCACTTC
CTCCACCGACTCAACCGCACCGACGATACCTCGGTATACAGTGAGCAGAAACTCCTGCCGAGCCTCAAGAAGAGGTGGAACGACCTGAAAAGCGTTCACT
CGAAAGTCCTACAGAAAGTCGTTCAGCGGTTGTACGACAACCTCTCGACGCTTCGCGGTCGCAAAGAGAATGGCTACCGCGTCGGGACGCTCAAGTGGAA
GGCTCCGGGCGAGTACCGCAGTTTCACCTACAGTCAATCCGGCTTCAAGCTCAAGAACACGGGCGGTCGGACTCGATTGTGGCTCTCGAAAATCGGTGAC
ATACCCCTCACCTTCCACCGCCACCTGCCCGACGACACCGAAATCAAGACCGTCACGGTCAAACACGAACCAACCGGCAAGTGGTACGCCATCCTTGGCG
TCGAAACCCCGGACGACCCGCCGGAGAAGCCGGAGAATCCCGAGAAGTGCGTCGGCATCGACGTGGGGATTCTCAAGTACGCTCACGACACCGACGGAAC
CGCCGTCGAATCGCTCAACTTGTCCGACGAGCGTGACCGCTTGGAACGCGCACAGCGCGACCTCTCGCGGAAACAGCACGGGTCTGCGAATTGGGAGAAA
CAGCGCCGTGTCGTGGCCGAGCGTCACGCCGACCTCAAGCGAAAGCGGCGGGACTTCCTCCACAAGTTGTCGAACCACTACGCGCGGGAGTACGACTTTG
TGGCAGTCGAAGACCTCGACGCGAAGGGGTTGGTCGAACTGCCGGGAAACTCACGCAACCGCGCAGGAGCGGCGTGGGGAACGTTCCTTCGGATGCTCGA
ATACAAGTGCGAACGCGAAGGAACACACTTCGTAGCGGTCAACCCGAGAGGGACGACCAAAGAATGCGCGTCCTGCGGTGTCTCGACGGACAAGCCGCTG
TGGGTCCGTGAACACTCCTGTCCCGCCTGCGGGTTCGAGGCGGACAGGGACGCGAACGCGGCGTGGAACATTCTTTCTCGCGGTATCAAGAAGCGGTTAG
GAGCGGGACGCTCCGAATCAATGTCCTCAGAATCGCCTTGCGATTCTGATGTGCGGACGAGAGCTCCGCTCTCGTCAACGCCTGTGGAGACTGCGCTCCC
TGTGGATACGTCCGTATCTGCAAAGCGCGTCGTTGAAACAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
141 bp	46 aa	153	293	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRHEIEGHEVIDGTAKATGNGAHVLVPKRWRGADVKVIRTTEPDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1281 bp	426 aa	302	1582	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLRPSDALEQQLAWTVDTCRQVYNHFLHRLNRTDDTSVYSEQKLLPSLKKRWNDLKSVHSKVLQKVVQRLYDNLSTLRGRKENGYRVGTLKWK
APGEYRSFTYSQSGFKLKNTGGRTRLWLSKIGDIPLTFHRHLPDDTEIKTVTVKHEPTGKWYAILGVETPDDPPEKPENPEKCVGIDVGILKYAHDTDGT
AVESLNLSDERDRLERAQRDLSRKQHGSANWEKQRRVVAERHADLKRKRRDFLHKLSNHYAREYDFVAVEDLDAKGLVELPGNSRNRAGAAWGTFLRMLE
YKCEREGTHFVAVNPRGTTKECASCGVSTDKPLWVREHSCPACGFEADRDANAAWNILSRGIKKRLGAGRSES

Blast result :

Comments

ISNje1 is 91% (TnpB) aa similar to ISHla22. The first ORF is passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission.
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.