ISfinder Mirror Site

ISHca1

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AOJK01000077	ND	Halorubrum californiensis	Halorubrum californiensis DSM 19288

DNA section

IS Length : 1549 bp

Ends

Left end : CTGAATTGAGGCGCTAAGGCCCCTTCCTCAAGGAGCGACCGAAGGGAGCGAGTAGGGAGGGGATACAGCGCCGCACAGTTCGCAAACACGTTCGACGCTT II struct. : Yes
Right end : TACGGACACCAATTCGGTGTCAGCAAAGCGCGTCGTGGAAGCAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
NNN	TTAC	NCACCAATTCGG	tcac

DNA sequence

CTGAATTGAGGCGCTAAGGCCCCTTCCTCAAGGAGCGACCGAAGGGAGCGAGTAGGGAGGGGATACAGCGCCGCACAGTTCGCAAACACGTTCGACGCTT
GGCCCACAGGCCCACGCATGGGCACATTTATGTTGATACAATGTTTAAACTCGGGTGATGGATAGGCACGAAATAGAAGGCCACGAAGTCATCGCAGGGA
CGGCGAAAGCCACCGGAAACGGCGCTCACGTCCTCGTCCCGAGGGACTGGCGTGGCGCAGACGTGAAAATCGTCCGAACAAGCGAACCCACCGACGAATA
GCCCTGACAGATGAACTACAACTACAGGTATCGGCTCCACCCGGCCGACGCGCTCAAAGACCAGTTAGCGTGGACTGTCGATACTTGTAGACAGGTCTAT
AACCACTTTCTCCACCGACTCAACCGAACCGACGACGCCTCGGCATACTCCGAGCAGAAGCGCCTCCCTGATCTCAAAGAGTGGTGGGATGACCTGAAAG
ACGTTCACTCGAAGGTTCTTCAAAAAGTCGTTCAGCGGCTATACGACAACCTCTCAACGCTCCGTGGTCGCAAAGAGAACGGCTACCACGTCGGAACACT
CAATTGGAAATCACCAAGCGAGTACCGCAGTTTCACCTACAGTCAATCCGGCTTCAAGCTCAAAGACACGAGCGGTCGGGCTCGACTGTGGCTCTCGAAA
CTCGGAGAAATCCCGCTCACCTTCCACCGTGACCTGCCCGACGACGCCGAAATCAAGACCGTCACGGTCAAGCAGGAACCCACCGGGAAGTGGTACGCCA
TCCTCGGGGTCGAGACTCCCGACGACCCGCCGGAGAAACCCGAGAATCCCGAGAAGTGCGTCGGTATCGACGTAGGGATTCTCAAGTACGCACACGATAC
TGACCGCACAGCTGTCGAATCACCCGACCTCTCAGACGAACGCGAACGGTTGGAAAGCGCACAGCGAGACCTCTCGCGGAAAGAACACGGCTCTGCGAAT
TGGGAGACACAACGCCGAATCGTGGCAAAGCGCCACGCCGACCTCAAGCGAAAGCGACGTGACTTTCTCCACAAGTTGTCGAACTACTACGCTACCGAAT
ACGACTTGGTTGCCGTGGAGGACTTGGATGCGAAGGGACTGGTTGAACTGCCGGGTAACTCGCGCAACCGAGCAGGTGTGGCATGGGGGACGTTCCTGCG
GATACTCGAATATAAGTGCGAACGCGAGGGGGCGCACTTCGTGGCAGTAGACCCGGCGGGGACGACTAAGGAGTGTGCGTCGTGTAGCGTGGAGACGGAG
AAGCCGTTGTGGGTGCGTGAACACTCTTGTCCTGCCTGTGGGTTTGAGGCAGACAGGGACGCGAATGCGGCGTGGAATATCCTTTCTCGCGGCATGAAGC
AGTTAGGAGCGGGACGCTCCGAATCAACGCCTGTGGAGACTGCGTTCCCTACGGACACCAATTCGGTGTCAGCAAAGCGCGTCGTGGAAGCAGGAAGCCC
CACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
144 bp	47 aa	158	301	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRHEIEGHEVIAGTAKATGNGAHVLVPRDWRGADVKIVRTSEPTDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1224 bp	405 aa	311	1534	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLHPADALKDQLAWTVDTCRQVYNHFLHRLNRTDDASAYSEQKRLPDLKEWWDDLKDVHSKVLQKVVQRLYDNLSTLRGRKENGYHVGTLNWK
SPSEYRSFTYSQSGFKLKDTSGRARLWLSKLGEIPLTFHRDLPDDAEIKTVTVKQEPTGKWYAILGVETPDDPPEKPENPEKCVGIDVGILKYAHDTDRT
AVESPDLSDERERLESAQRDLSRKEHGSANWETQRRIVAKRHADLKRKRRDFLHKLSNYYATEYDLVAVEDLDAKGLVELPGNSRNRAGVAWGTFLRILE
YKCEREGAHFVAVDPAGTTKECASCSVETEKPLWVREHSCPACGFEADRDANAAWNILSRKQLGAGRSESTPVETAFPTDTNSVSAKRVVEAGSPTLKRE
PSGER

Blast result :

Comments

ISHca1 is 91% (TnpB) aa similar to ISHla22. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (20115) Direct submission.
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.