ISfinder Mirror Site

ISHarch19

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP002988	ND	Halophilic archaeon	Halophilic archaeon DL31

DNA section

IS Length : 1624 bp

Ends

Left end : CTTCCTGCAAGGAGGGAATCCGACAACTACTGAACTAACCACGTTCTGACGCTCAGACTGAATGCCGACCGTTATCTTTAACTTACGCTACTTCTTACGT II struct. : No
Right end : ATTCCCACCGTGTGGGAAGCCCCGTCCCCGTCGTCGTCGGGCTGCGCCCGACTGCCTGAGGGACGTAGTCCCTCTCTGTTTACGACGGGGAGGATGTCAC II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GCAGGCGCGTCC	CCAT	CGCGCTCGTACT	tcac

DNA sequence

CTTCCTGCAAGGAGGGAATCCGACAACTACTGAACTAACCACGTTCTGACGCTCAGACTGAATGCCGACCGTTATCTTTAACTTACGCTACTTCTTACGT
TTTGGTGGATGAGAGCTGACTTCGATATGGAGCGAGGCGGGCCACTTCACGAAGCCGTGAAGCAATACGCCCGCGATAACGGGATACGCCACTCACGAGC
GTACCCTGAACTCCTCCGCAAAGCACTCGAATCCGAAGGCTACGATGTCAACGACTCCGACAGCGAATAAGACGCTGGAAGCCACTCTCGCACCGCCCAC
ACGGTGCAAAGAGCAACGCCTCCAGCAAACGCTGTCTGAATACCGAGACGCACTCGAAGACGCCTTCGAGCAAGACTGCACGACGATGAGCGCCACGAAC
GACGTGGTGACACCGTACAACCTACCGTATCAAGCAAAAGACGCCCTCAAATCCTACGTTCCAAAACTTCACAAGACGTACAAGGCGAAGGAATTGGATG
ACGAACATCCCCTCAGGTTCGTGAATCGGGCCGGGAAGTTCGACCGAGACTCCTCGCGCGAGTACGAAATCTGCTGGAACGTTCCGCAACCCGGTCGCGG
AACCAACTTCTGGATACTACTCCGACTCAATCCAGAGCAGCAAGGATTGTGGGATGAAATGCTCGATGATGAGTCGAGTACCAACGTAGGCGAACTTCGC
TTGCAGAAACACCGGAAGGCGTGGACACTCCACGTCACCGTCGAATACGAAATCGAGAACACCTCCGAACTACCCGAGAACCCGACACGGGTTGGATTCG
ATATTGGTGAGTCGATGTTGGTCGCGGGCTGTGCCCTCCAACACGACACTTCTACGAAACCACTGTTAATCAACGGGAAGGAAGCCAAGCGAATCCGCAA
AGAGATGTTCACGACCCTTCGTCGCCTCCAAGAGCGAGACGCTTCCGAGTGGCGCGTTGAGGAACGCTTTTCGTACTACCAGAACCGACTCACAGACATT
ATCGAGAAGGCGTCTCGTGAGTCTGTGGAGTACGCCCGTCAATTCGAGAATCCCGTCATCGTGATGGAGGACTTGGCGTACATTCGTGAGTCACTGGACT
ACGGGAAGTACATGAATCGACGCCTCCACGCGTGGGCCTTTGCTCGCTTACAGGGACGCATCGAGGACAAGGCAAAGGACGCGGGTATTCCAGTCCGATA
CATTCACCCTCAATACACGAGTAAGACGTGCCACTCGTGCAAGCACATCGGGTATCGGCCTCGGCAGGCCGAGTTCAAGTGCAAGAACCCAGACTGCCAC
GTATCGACGTTCCAAGCAGATATTAACGCGAGTGCGAATATCGCACGGCGCGTAGACCCGTGGGGAGAGAGCCTACCAGTGAAACGGGTAGGCGATGACT
CGCCACAGGACGGGAGCCGTAGTGACACGGCCACGACTCAGTGTGAGCAGAGCGAGACACCCTCGCAGATGACACTCACAACGTTTCAAGAGTCGAAACC
CACTGCGAGAGACGACTAACTGGCATTCCCACCGTGTGGGAAGCCCCGTCCCCGTCGTCGTCGGGCTGCGCCCGACTGCCTGAGGGACGTAGTCCCTCTC
TGTTTACGACGGGGAGGATGTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
161 bp	53 aa	109	269	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRADFDMERGGPLHEAVKQYARDNGIRHSRAYPELLRKALESEGYDVNDSDSE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1275 bp	424 aa	245	1519	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MSTTPTANKTLEATLAPPTRCKEQRLQQTLSEYRDALEDAFEQDCTTMSATNDVVTPYNLPYQAKDALKSYVPKLHKTYKAKELDDEHPLRFVNRAGKFD
RDSSREYEICWNVPQPGRGTNFWILLRLNPEQQGLWDEMLDDESSTNVGELRLQKHRKAWTLHVTVEYEIENTSELPENPTRVGFDIGESMLVAGCALQH
DTSTKPLLINGKEAKRIRKEMFTTLRRLQERDASEWRVEERFSYYQNRLTDIIEKASRESVEYARQFENPVIVMEDLAYIRESLDYGKYMNRRLHAWAFA
RLQGRIEDKAKDAGIPVRYIHPQYTSKTCHSCKHIGYRPRQAEFKCKNPDCHVSTFQADINASANIARRVDPWGESLPVKRVGDDSPQDGSRSDTATTQC
EQSETPSQMTLTTFQESKPTARDD

Blast result :

Comments

ISHarch19 is 95% (TnpB) aa similar to ISHli14. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Dyall-Smith,M., Cavicchioli,R. and Woyke,T. (2011) Direct submission to GenBank