ISfinder Mirror Site

ISHarch18

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP002988	ND	Halophilic archaeon	Halophilic archaeon DL31

DNA section

IS Length : 1608 bp

Ends

Left end : GCTTCCTTAATTCAAGCAGGGAATCCCGGCCTTCAGGCCGGGAGTGAATGCGACACGGTACTGAGTGGCCGCTACTCGGTGGCCGGGCCGGAATCCAACT II struct. : Yes
Right end : CGCCGGATTCCCACCGTGTGGGAAGCCCCGTCGTCGTCGGGCTACGCCCGACTGCCTGAGGGACGTAGTCCCTCTCTATTTACGACGGGGAGGATGTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
ACCTCGTCCGGG	TCGT	GCGCGTTCCTCG	tcac
CTGGGTTGGACA	TCGT	GCGAGAGATTCC	tcac

DNA sequence

GCTTCCTTAATTCAAGCAGGGAATCCCGGCCTTCAGGCCGGGAGTGAATGCGACACGGTACTGAGTGGCCGCTACTCGGTGGCCGGGCCGGAATCCAACT
CCCGTTGAGCGTGGAGTAACATCCCGCGCCACGTCAGGCCGTGACGCTTCTTCGTCTCTTTCAGACTCTCGTACTGTTCCTCGTCCACCTCGATGTTGAT
GTTCTTCACATCTCATGGTTTGTGACCAATGGTGTTAAGTTTAACTAATACTATTGTAGCATTGTGTCCGAGACGGTGACAAAGACGCTACAGGCCACAC
TCGCCACGCCCACCACGGGCAAAGAGCAACGCCTACAGCGACTCTTGGACACCTACCGAACCGCACTCCACGACGCCTTCGACAACAGGGCGGACACAAT
GTCTGCCGTCAACGACGTTGTGACGCCCTACGACCTGCCGTACCAAGCCAAAGACGCGCTCAAATCCTACGTCCCGAAACTCCGTGACACGTACAACGCC
GAGGAGTTGGACGACGAGCATCCGCTTCGACTCGTCAACCGGGCCGCAACGTTCGACTACTCCGACGAGCGCGAACACGGCTTCGTGTGGCAGGCCCCGC
AACCCGGACGCGGCACGAACTTTTGGATTCCGCTTCGGATTAACCCCGAACAGGAATCGCTATGGTTCGACCTGCTCTCCGAAGACGCGAAAGCGGGCGC
GTTGCGGCTACAGCGACACCGCACGTCGTGGGAGTTGCACGTCACCGTCGAATACGAGGTCGAAGAACCGACCGAATCGGACGACGAGACGTACATCGGT
TTCGACGTAGGCGAGAGCGCGTTGATAACGGGCTGTGCCCTCAAACGCGACGTACCACGGAAGCCGATGCTCGTCAGCGGCAGTCGAGCGCGACACCTCC
GCAAAGAGATGTTCACGACGCTCCGACGGCTTCAGTCGAGAGACGCCGCCGAGTGGCGCGTAGACGAGCGATTCGACCACTACCAGAACGCCCTCACCGA
CATCATGGAGAAGGCGTCTCGGGAAGCCGTGGAATACGCCGATTCCTTCGAGAACCCGGTTATCGTCCTCGAAGACCTGTCGTACATCCGCGAACGGTTG
GACTACGGGAAGTTCATGAACCGACGCCTCCACGCATGGGCGTTCGCCCGGTTACAGGGTCGAATCGAGGACAAAGCGACCGAGGCGGGCATTCGCGTCG
AGTACGTCAACGCGGCGTACACGTCCCAAACGTGCCACGCCTGCGGCTGTCTCGGTCGGCGGAGCCAGCAAGCAGAGTTCGTGTGTCCGCACGACGACTG
CCACGTATCGGAGTTCCAAGCAGATATTAACGCGGCGGCGAACATCGCCGGTCGCGCTAACCCGTGGGGTGAGAGCGTTCCTTGGAAACCGGAACGCGAT
GACACGCCACAGGACGGGAGCGGTAGTGACACCGCCACAGTCCACCGAGAGACGAGTCCGAGAACCGGACAGATGACGCTCTCGGCGTACTCGGACTGAA
ACCTGCCACGCCGGATTCCCACCGTGTGGGAAGCCCCGTCGTCGTCGGGCTACGCCCGACTGCCTGAGGGACGTAGTCCCTCTCTATTTACGACGGGGAG
GATGTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
138 bp	45 aa	209	72	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKNINIEVDEEQYESLKETKKRHGLTWRGMLLHAQRELDSGPATE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1236 bp	411 aa	264	1499	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MSETVTKTLQATLATPTTGKEQRLQRLLDTYRTALHDAFDNRADTMSAVNDVVTPYDLPYQAKDALKSYVPKLRDTYNAEELDDEHPLRLVNRAATFDYS
DEREHGFVWQAPQPGRGTNFWIPLRINPEQESLWFDLLSEDAKAGALRLQRHRTSWELHVTVEYEVEEPTESDDETYIGFDVGESALITGCALKRDVPRK
PMLVSGSRARHLRKEMFTTLRRLQSRDAAEWRVDERFDHYQNALTDIMEKASREAVEYADSFENPVIVLEDLSYIRERLDYGKFMNRRLHAWAFARLQGR
IEDKATEAGIRVEYVNAAYTSQTCHACGCLGRRSQQAEFVCPHDDCHVSEFQADINAAANIAGRANPWGESVPWKPERDDTPQDGSGSDTATVHRETSPR
TGQMTLSAYSD

Blast result :

Comments

ISHarch18 is 96% (TnpB) aa similar to ISNamo26. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Dyall-Smith,M., Cavicchioli,R. and Woyke,T. (2011) Direct submission to GenBank