ISfinder Mirror Site

ISHwa31

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
ARPY01000183	ND	Haloquadratum walsbyi	Haloquadratum walsbyi J07HQW2

DNA section

IS Length : 1535 bp

Ends

Left end : AAATCATAGGCGCTAGGACCCCTTCCTCAAGGAGCGAGCGAAGCGAGTGAGTATGGAGGGGATACAGCGCCGTCATCATCGAAGCAATCATATACACAGA II struct. : Yes
Right end : TTGTGGATGGAAAGCGCGTTATCGAATCAGGAAGCCCCACCCTCCACCCTCGGGGAACGAACCGTGAAAGCGGTGAGTGAGTAGGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
AATTATACTCTG	TTATC	TTAGATGGCATA	tcac

DNA sequence

AAATCATAGGCGCTAGGACCCCTTCCTCAAGGAGCGAGCGAAGCGAGTGAGTATGGAGGGGATACAGCGCCGTCATCATCGAAGCAATCATATACACAGA
GTATATATTCCCTATCACGATGGAACGTACAATGGTAACGCTCAGAGAAGACCAGTCAGAGTGGCTCGAGGAGAATCAGCTTAATCTCTCGGCATTCCTT
CGCGATAGACTCGACGACAAGATTGAGCAACGCAACAACCAGTAATATCATGAACTACGCCTACAGGTATCGAATCCATCCGACTGAGTCCCAGCGAGAG
ACATTGGACCATCATCTCGATACCTGTCGGCAGATATACAACCACACGCTTCACGAGCTCAATAATATTCCCGAATCAGCTGGAACAGTCAACCAGCGTG
TGAATAAGGTAATTAAACAACTGCCTGACCTGAAGGAGTGGTGGGACGAGCTCACTGAGCTGTACTCAACCGTCTCCCAAGACGCAGCAAAACGCGTCGC
AAAAAACATCATCCGTCTCTCTGGTGCCAAACAGAACGGTCTCAAGGTTGGCAGTCTCAATTGGAAGTCACCACGCGAGTATCAGAGTTTCACCTACGTG
CAGTCTGGCTTCGAGTTCGACAACAAGAACGGTCAGACTGTGCTGTCACTCTCGAAACTCGCAGACATTCCTCTTAGCTATCATCGGGACATATCTGAGA
CTGTCAATATTAAAAGAGTCACTCTAAAGAGAGAACCAACAGGTGAGTGGTACGCTTCCTTTGCAGTCGATGAGAAAGAAGAGCCAGCAAAACCAGACGA
CCCACAGCGGTGTGTCGGAATCGATGTTGGGATTCTCAAATACGCTCAAGACACTGACGGTCGTGCTGTCGGATTTCCTGACCTGCAGGATAACCGAGAG
CGTCTCAGACGCGAGCAACGGGCTCTCTCGCGAAAAGAGCAGGGCTCGAACAACTGGGAGAAACAGCGACAGACAGTTGCAGAATACTATCAGACGCTTC
GACGGAAATGTCACGACTTCCTGCACAAACTGTCGAACTATTATGCCACCCAGTACGATTTGGTGGCTGTCGAGGATTTGGATGTGAAGTCGATGCTGAA
GTCGTCTGGGAACAGTCGGAAAACGGCTTTCGCGGCTTGGGACACATTCACCACGATGCTCGAATACAAGTGCAAGCGTGAAGGCACTCACTTTGTCGAA
GTTGAGCCAGCAGGGACGACGAAAGAATGTGCGTCTTGTAGCGTGGAGACCAAGAAGCCGTTATGTGTGAGAGAACACTCGTGTCCAGCGTGTGGATTCG
AGATGGATAGAGACGCGAACGCGGCGATTAATATTCTGTCTCGCAGCTTCAAGAAGCTAGGACTGGGACAGTCCGAAGACACAACGCCTGTGGAGACTGC
GCTCCCTATGTTCACCTCATCTGGCAGTGTTGATGTTGTGGATGGAAAGCGCGTTATCGAATCAGGAAGCCCCACCCTCCACCCTCGGGGAACGAACCGT
GAAAGCGGTGAGTGAGTAGGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
126 bp	41 aa	120	245	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MERTMVTLREDQSEWLEENQLNLSAFLRDRLDDKIEQRNNQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1266 bp	421 aa	250	1515	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYAYRYRIHPTESQRETLDHHLDTCRQIYNHTLHELNNIPESAGTVNQRVNKVIKQLPDLKEWWDELTELYSTVSQDAAKRVAKNIIRLSGAKQNGLKV
GSLNWKSPREYQSFTYVQSGFEFDNKNGQTVLSLSKLADIPLSYHRDISETVNIKRVTLKREPTGEWYASFAVDEKEEPAKPDDPQRCVGIDVGILKYAQ
DTDGRAVGFPDLQDNRERLRREQRALSRKEQGSNNWEKQRQTVAEYYQTLRRKCHDFLHKLSNYYATQYDLVAVEDLDVKSMLKSSGNSRKTAFAAWDTF
TTMLEYKCKREGTHFVEVEPAGTTKECASCSVETKKPLCVREHSCPACGFEMDRDANAAINILSRSFKKLGLGQSEDTTPVETALPMFTSSGSVDVVDGK
RVIESGSPTLHPRGTNRESGE

Blast result :

Comments

ISHwa31 is 77% (TnpB) aa similar to ISNma21. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015)
2] Podell,S., Ugalde,J.A., Narasingarao,P., Banfield,J.F., Heidelberg,K.B. and Allen,E.E.(2013) PLoS ONE 8 (4), E61692