ISfinder Mirror Site

ISHti10

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
HF571520	ND	Halorhabdus tiamatea	Halorhabdus tiamatea SARL4B

DNA section

IS Length : 1387 bp

Ends

Left end : CAGAATGAGGCGAGAATGCCCCTTCCTCAAGGAGCGAGTATTGCGAGTGAGTAGGGAGGGGATGAATCGCCGCACAAGATACAAACCATTAAGTAAATAT II struct. : Yes
Right end : TACGGACACCACTTCGGTGTCTGCAAAGCGCGTCATCGAAACAGGAAGCCCCACCCTCAACGAAGCCACAACAGTGGCTGAGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCCCGGCTGGCT	TTAC	GCGGTCGTGAGT	tcac

DNA sequence

CAGAATGAGGCGAGAATGCCCCTTCCTCAAGGAGCGAGTATTGCGAGTGAGTAGGGAGGGGATGAATCGCCGCACAAGATACAAACCATTAAGTAAATAT
ATCAACAATCAATAGACTGCGATGGAGTACAGTCACCGTTACCCTGCATACCCGACACAAGAGGTAGCGGCTGAACTGGAACATCATATCGACATTCATC
GCCAAGCGTACAACTACACTCTGTACGAATACGAGAACGTGGATGCCGACGACATCGGCTCCGCGTACAAACACCACTACCGACTTCCCGATTGGAAAGA
CGAGTTCTCCGTCTTCTCGGAGGTCAACTCGAAGGCTTTGCAACGAACCGTCACACGGTTCTACGACAACCTCGACGGACTCTCCGAACAGAAAGAGAAC
GGTCGAACGGTCGGGAAACTCAATTGGAAGTCACCGAGAGAGTTCCAAAGTATGACGTTTTCGCAGTCCGGCTTCGAACTCAAAAACACGAGTGGCCGAC
ACGCGACACTCTGGCTCTCCAAAATCGGTGACATTCCGATTCGCTATCACCGGGAGATCCCTGACGAAGCGGATATCAAAGAAGTCACGGTCAAGAAGGA
GACAACCGGTGACTGGTTCGTCTCGTTCGGACTGGAAACCGACGAAACTGACCTACCCGAGAAGCCCGACGTGGATTCACTGAACGTGAACAATAGCGTC
GGGATTGACCTCGGCATCCTGAACTACATCCACACCTCGAACGGTAAAACCGTGGACTGGCTCGACCTCGAAGACGAATACGAGCGTCTGCGCCGTGAAC
AACGCAAGTTGTCGCACAAGGAGCAAGGGTCGAATAACTACGAGAAACAACGCCGGGAAGTGGCGAAGGTCAAGCGTCACATCCGGCGGAAGGTGCTGGA
CTACCAGCACAAGATTACGACATGGCTTGTCCGCGAATACGACGCCGTATTCGTCGAGGACTTGAACGTGAAGGGGATGCTGAAACAGTCGCATAATGCT
CAGAACAAGCAGGATGCGGCGTGGCGACAGTTCATCACCCTTCTCGAATACAAGGCTGGCCTGTATGGCTGTCACGTCAAGCAGGTTGAAGCACGAGGGA
CGACGACGGAATGTGCGTCGTGCGGTGTGGAGACTGCGAAGCCGATTTGGGTTCGGGAGCACTCTTGCCCGAGTTGTGGGTTCGAGTGTGACCGGGATGC
GAATGCGGCGATGAACGTACTGCAACGCGGTTTTTCTGAACTAGGGCTGGAATGGCCCGAATCCACGTCTGTGGAGACTGCGCTCCCTACGGACACCACT
TCGGTGTCTGCAAAGCGCGTCATCGAAACAGGAAGCCCCACCCTCAACGAAGCCACAACAGTGGCTGAGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1251 bp	416 aa	122	1372	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MEYSHRYPAYPTQEVAAELEHHIDIHRQAYNYTLYEYENVDADDIGSAYKHHYRLPDWKDEFSVFSEVNSKALQRTVTRFYDNLDGLSEQKENGRTVGKL
NWKSPREFQSMTFSQSGFELKNTSGRHATLWLSKIGDIPIRYHREIPDEADIKEVTVKKETTGDWFVSFGLETDETDLPEKPDVDSLNVNNSVGIDLGIL
NYIHTSNGKTVDWLDLEDEYERLRREQRKLSHKEQGSNNYEKQRREVAKVKRHIRRKVLDYQHKITTWLVREYDAVFVEDLNVKGMLKQSHNAQNKQDAA
WRQFITLLEYKAGLYGCHVKQVEARGTTTECASCGVETAKPIWVREHSCPSCGFECDRDANAAMNVLQRGFSELGLEWPESTSVETALPTDTTSVSAKRV
IETGSPTLNEATTVAE

Blast result :

Comments

ISHti10 is 94% aa similar to ISHwa25.

References

1] Pfeiffer F. (2015) Direct submission to ISfinder
2] Werner,J., Ferrer,M., Michel,G., Mann,A.J., Huang,S., Juarez,S., Ciordia,S., Albar,J.P., Alcaide,M., La Cono,V., Yakimov,M.M., Antunes,A., Taborda,M., da Costa,M.S., Hai,T., Glockner,F.O., Golyshina,O.V., Golyshin,P.N. and Teeling,H. (2014) Environ. Microbiol (in press)