ISMno25
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001349 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 |
DNA section
IS Length : 1518 bp
Ends
IR Length : 10
IRL : AGATCCCATGAGGGCGGCGCGAGACGGTTAGTCTGGTCTCCGGTGCACGA
IRR : ----CACATGAGGGCGCAGTCGTCAAGCTACCTCATGGCGGGTTCCTCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGCGTCGAC | AGGCCGGACA | 0 |
DNA sequence
AGATCCCATGAGGGCGGCGCGAGACGGTTAGTCTGGTCTCCGGTGCACGACCTTGAGGATCCTCGCTATGACGCAGATGCCCAGCTCATCGTCTCCGACG
CCTGATTACGAGCGCACGCTCGTCCTCGCAATCGAGTTGAGCAACGTGAGTTGGGTCTTGGCCGCTCAGATCCCTGGCTTGCCACGCCTGAAGGCCAAGC
GCAGCGTCGCGCCGGCTCCCGAGGCCCTGTTGGCGGCCATCGAAGGTTATCGCGAGCGAGCCCGCGCGGCAGGACGCGACGTCGAACGCGTGATCGCGAT
CTACGAGGCTGGCTGGTCGGGCTTCTGGCTGGCGCGCTGGCTGGGCATGCGCGGGATTGAGACGCATGTCGTCCAGCCCGCGAGCGTGCCGGTGGATCGC
CGGGCGCGGCGCGCCAAGTCCGACGGGATCGATGCCGAACTGCTGCTGCGAACGCTGCTTGCCTGGCTGCGCGGTGAGCCACGCGTGTGCTCGATGGTGC
CCGTCCCGAGCGAGGCCGACGAGGACGCCCGACGGTGCGTGCGCGAGCGGACGGAGCTCGTGACCGAGCGGGTCGCCCTGGTGAACCGCATTGGTGCCGT
GCTGGCGACGTTGGGCGTGCGCGACTACAACCCCTTGCTGCGGAACCGGCGCGAGCGGCTCGATGCGCTGCGCACCGCGCTCGGGACGCCGCTGCCGGCG
CATGCCCGCGCCAAGATCGGACGCCTGCTCGACCGCCTGGATCTGGTGTGCGCGCAGATCACGGCCCTGGAGCGGGAGCGCGACGCCGTCCTCGACCAGG
AGGCGCCGGACAGGGCCGGCGCGATGATCCAGCAACTGAGCACGTTGCGCGGCATCGGTGTGCAGAGCGCTACCATCCTGGTGCGCGAGGCGTTCGTGCG
CGTGTTCGCCAACGCCAAAGCGCTCGGCGCTTACGCAGGCTTGGCCGCGACCCCCTACAGCAGCGGCGGGACGGTGCGCGAGCAGGGCATCGGGAAGGCC
GGGAACCGGCGGCTGCGGACCGTCATGGTCGAACTCGCTTGGCTCTGGCAACGCTATCAGCCCGGCTCCGCCCCGGTGAACTGGTTCCGTACGCGCGTCA
GCGGTGCAGGCGCGCGTGGCCGCAAGGTGATGGTGGTGGCCCTGGCGCGCAGGCTCCTCATCGCTCTGTGGCGGTTCGCCACCCAGGGCGTTCTGCCGGA
GGGTGCGGTCCTGAAGCCCGCGTCCTGATCGCCTGCGAAGATCGGGTATGAGCCGTCGGTCGGCTCGGCCCTCAGGATCCAAGAGGGCGTCGCCGTTGTC
CGGCTTGGCGCACGAACGCCGTCAAATAGTGGCGCCACCCTCCTGGAGCTCTCCCGTCCCGGATGCGGGACAGTGGTGGGACATGTCCCGCCGGATGTGA
GGTTTGCAGGGAAGCCCTGCGCTGAAGAGAGCTCCATCTTGGACAACAATTCCCCTGTGCCCCGCAAAGTTCTGGAGGAACCCGCCATGAGGTAGCTTGA
CGACTGCGCCCTCATGTG
CCTGATTACGAGCGCACGCTCGTCCTCGCAATCGAGTTGAGCAACGTGAGTTGGGTCTTGGCCGCTCAGATCCCTGGCTTGCCACGCCTGAAGGCCAAGC
GCAGCGTCGCGCCGGCTCCCGAGGCCCTGTTGGCGGCCATCGAAGGTTATCGCGAGCGAGCCCGCGCGGCAGGACGCGACGTCGAACGCGTGATCGCGAT
CTACGAGGCTGGCTGGTCGGGCTTCTGGCTGGCGCGCTGGCTGGGCATGCGCGGGATTGAGACGCATGTCGTCCAGCCCGCGAGCGTGCCGGTGGATCGC
CGGGCGCGGCGCGCCAAGTCCGACGGGATCGATGCCGAACTGCTGCTGCGAACGCTGCTTGCCTGGCTGCGCGGTGAGCCACGCGTGTGCTCGATGGTGC
CCGTCCCGAGCGAGGCCGACGAGGACGCCCGACGGTGCGTGCGCGAGCGGACGGAGCTCGTGACCGAGCGGGTCGCCCTGGTGAACCGCATTGGTGCCGT
GCTGGCGACGTTGGGCGTGCGCGACTACAACCCCTTGCTGCGGAACCGGCGCGAGCGGCTCGATGCGCTGCGCACCGCGCTCGGGACGCCGCTGCCGGCG
CATGCCCGCGCCAAGATCGGACGCCTGCTCGACCGCCTGGATCTGGTGTGCGCGCAGATCACGGCCCTGGAGCGGGAGCGCGACGCCGTCCTCGACCAGG
AGGCGCCGGACAGGGCCGGCGCGATGATCCAGCAACTGAGCACGTTGCGCGGCATCGGTGTGCAGAGCGCTACCATCCTGGTGCGCGAGGCGTTCGTGCG
CGTGTTCGCCAACGCCAAAGCGCTCGGCGCTTACGCAGGCTTGGCCGCGACCCCCTACAGCAGCGGCGGGACGGTGCGCGAGCAGGGCATCGGGAAGGCC
GGGAACCGGCGGCTGCGGACCGTCATGGTCGAACTCGCTTGGCTCTGGCAACGCTATCAGCCCGGCTCCGCCCCGGTGAACTGGTTCCGTACGCGCGTCA
GCGGTGCAGGCGCGCGTGGCCGCAAGGTGATGGTGGTGGCCCTGGCGCGCAGGCTCCTCATCGCTCTGTGGCGGTTCGCCACCCAGGGCGTTCTGCCGGA
GGGTGCGGTCCTGAAGCCCGCGTCCTGATCGCCTGCGAAGATCGGGTATGAGCCGTCGGTCGGCTCGGCCCTCAGGATCCAAGAGGGCGTCGCCGTTGTC
CGGCTTGGCGCACGAACGCCGTCAAATAGTGGCGCCACCCTCCTGGAGCTCTCCCGTCCCGGATGCGGGACAGTGGTGGGACATGTCCCGCCGGATGTGA
GGTTTGCAGGGAAGCCCTGCGCTGAAGAGAGCTCCATCTTGGACAACAATTCCCCTGTGCCCCGCAAAGTTCTGGAGGAACCCGCCATGAGGTAGCTTGA
CGACTGCGCCCTCATGTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1161 bp | 386 aa | 68 | 1228 | + | No |
Chemistry : DEDD
ORF sequence :
MTQMPSSSSPTPDYERTLVLAIELSNVSWVLAAQIPGLPRLKAKRSVAPAPEALLAAIEGYRERARAAGRDVERVIAIYEAGWSGFWLARWLGMRGIETH
VVQPASVPVDRRARRAKSDGIDAELLLRTLLAWLRGEPRVCSMVPVPSEADEDARRCVRERTELVTERVALVNRIGAVLATLGVRDYNPLLRNRRERLDA
LRTALGTPLPAHARAKIGRLLDRLDLVCAQITALERERDAVLDQEAPDRAGAMIQQLSTLRGIGVQSATILVREAFVRVFANAKALGAYAGLAATPYSSG
GTVREQGIGKAGNRRLRTVMVELAWLWQRYQPGSAPVNWFRTRVSGAGARGRKVMVVALARRLLIALWRFATQGVLPEGAVLKPAS
VVQPASVPVDRRARRAKSDGIDAELLLRTLLAWLRGEPRVCSMVPVPSEADEDARRCVRERTELVTERVALVNRIGAVLATLGVRDYNPLLRNRRERLDA
LRTALGTPLPAHARAKIGRLLDRLDLVCAQITALERERDAVLDQEAPDRAGAMIQQLSTLRGIGVQSATILVREAFVRVFANAKALGAYAGLAATPYSSG
GTVREQGIGKAGNRRLRTVMVELAWLWQRYQPGSAPVNWFRTRVSGAGARGRKVMVVALARRLLIALWRFATQGVLPEGAVLKPAS
Blast result :
Comments
ISMno25 is 62% aa similar to ISAzs35.
ISMno25 is inserted in another IS : ISMno26 (IS110 family)
ISMno25 is inserted in another IS : ISMno26 (IS110 family)
References
1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.