ISNaoc7
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP003929 | ND | Natronococcus occultus | Natronococcus occultus SP4 |
DNA section
IS Length : 1388 bp
Ends
Left end : ATTGGTCACCGAGGCGAATACCCCGACCCACCGTGGTCGGGGATGAAGCCGACAACTCGTGACACAATCCATTAAGTAAACATATCTCTACCCAACAGAC II struct. : Yes
Right end : TGACTTTCGAGAGGTCTCTGCAAGTCACGTCATCGAAACAGGAAGCCTGCCCTCGTGAGAGCAGGATTCCCCGCGCCACCGTGCGCGGGGTAACATTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| CGGCCTGTTACT | TTTAT | TGGGATTCGTAC | tcaa |
| TGTCCCGGACGG | TTTAT | ACCGGACTGCCA | tcaa |
DNA sequence
ATTGGTCACCGAGGCGAATACCCCGACCCACCGTGGTCGGGGATGAAGCCGACAACTCGTGACACAATCCATTAAGTAAACATATCTCTACCCAACAGAC
AGCGATGGAATACAGTCACCGCTACCGCGCCTACCCGACACAAGAGGTAGCGGAGCGACTGGAACACCATCTCGACGTTCATCGCCAACTCTACAACCAC
GTCCGCTGGGACTACCAGAACAGCCCACAGGACGACAAACCGAGTGAATACGACCAGAACAACAAGCTTCCCGAGTGGAAGCAAAAGTGGTCACTATTCG
GTGAACTACACTCGAAGGCCGCGCAAGCCACCGTCGCACGCTTCCACCACAACCTCACCGTCCTCAGCGAACTCAAAGAGAAGGGATACACCGTCGGTCG
GCTCAAGCGGCAAGCACCCAGCGAGTACCGAAGCGTGACGTACAACCAGTCTGGTTTCGACCTCGATGAAAAGAGGGGCCACGACAACTACGCTTACGTC
CGCTTCAGCAAAATCGGGTGGGTGAAAATCCGGTATTCCCGTCCGATTCCCGACCACGCCACCATCAAAGAAGTCTCGTTCAAAAAGGAGACGACCGGCG
AGTGGTTCGTCTCGTTCGGGTTGGAAACCGACGAAGCTGGCCTACCGGAGAAACCCGACGGTGACTCGCTGGACGCGAGTAACAGCGTCGGAATTGACCT
CGGCATCCAGAACTACGTCCACACCAGCGATGGCAAGACCGTGGATTGGCTCGATCTTGAAGACGACTACGAGCGATTGCGCCGCGAACAACGCAAGTTG
TCTCGCAAGGAGAAGGGGTCAAACAACTACGAGAAGCACCGCCGAGAAGTGGCGAAGGTCAAGCGTCACATCAAGCGGAAAGTGCTGGACTACCAGCACA
AGAGTACGACGTGGCTCGTCAAAGAGTACGATGCCGTGTTCGTGGAAGACCTCAACGTCGCTGGAATGCTTCAAGAGGATGGGAACGCTCGGAACAAGCA
GGACGCGGCGTGGCGACAGTTCATTACCCTCCTTGACTACAAGGCCGACCTGTACGGGACGCACGTCGTAGAGGTCGAAGCACGAGGCACCACCAAAGAA
TGTGCATCGTGCGGTGTGGAGACAGCGAAGCCCATCTGGATTCGTGAACACTCCTGTCCGAGTTGTGGATTCGAGACGGACAGGGACGCGAACGCGGCGA
TGAACGTCCTCAAGCGCGGCTTTGCTGAGTTAGGACTGGGATGGCCCGAAGAAACGCCTGTGGAGACTGTGACCGCTACGGACGCCGATGACTTTCGAGA
GGTCTCTGCAAGTCACGTCATCGAAACAGGAAGCCTGCCCTCGTGAGAGCAGGATTCCCCGCGCCACCGTGCGCGGGGTAACATTCAA
AGCGATGGAATACAGTCACCGCTACCGCGCCTACCCGACACAAGAGGTAGCGGAGCGACTGGAACACCATCTCGACGTTCATCGCCAACTCTACAACCAC
GTCCGCTGGGACTACCAGAACAGCCCACAGGACGACAAACCGAGTGAATACGACCAGAACAACAAGCTTCCCGAGTGGAAGCAAAAGTGGTCACTATTCG
GTGAACTACACTCGAAGGCCGCGCAAGCCACCGTCGCACGCTTCCACCACAACCTCACCGTCCTCAGCGAACTCAAAGAGAAGGGATACACCGTCGGTCG
GCTCAAGCGGCAAGCACCCAGCGAGTACCGAAGCGTGACGTACAACCAGTCTGGTTTCGACCTCGATGAAAAGAGGGGCCACGACAACTACGCTTACGTC
CGCTTCAGCAAAATCGGGTGGGTGAAAATCCGGTATTCCCGTCCGATTCCCGACCACGCCACCATCAAAGAAGTCTCGTTCAAAAAGGAGACGACCGGCG
AGTGGTTCGTCTCGTTCGGGTTGGAAACCGACGAAGCTGGCCTACCGGAGAAACCCGACGGTGACTCGCTGGACGCGAGTAACAGCGTCGGAATTGACCT
CGGCATCCAGAACTACGTCCACACCAGCGATGGCAAGACCGTGGATTGGCTCGATCTTGAAGACGACTACGAGCGATTGCGCCGCGAACAACGCAAGTTG
TCTCGCAAGGAGAAGGGGTCAAACAACTACGAGAAGCACCGCCGAGAAGTGGCGAAGGTCAAGCGTCACATCAAGCGGAAAGTGCTGGACTACCAGCACA
AGAGTACGACGTGGCTCGTCAAAGAGTACGATGCCGTGTTCGTGGAAGACCTCAACGTCGCTGGAATGCTTCAAGAGGATGGGAACGCTCGGAACAAGCA
GGACGCGGCGTGGCGACAGTTCATTACCCTCCTTGACTACAAGGCCGACCTGTACGGGACGCACGTCGTAGAGGTCGAAGCACGAGGCACCACCAAAGAA
TGTGCATCGTGCGGTGTGGAGACAGCGAAGCCCATCTGGATTCGTGAACACTCCTGTCCGAGTTGTGGATTCGAGACGGACAGGGACGCGAACGCGGCGA
TGAACGTCCTCAAGCGCGGCTTTGCTGAGTTAGGACTGGGATGGCCCGAAGAAACGCCTGTGGAGACTGTGACCGCTACGGACGCCGATGACTTTCGAGA
GGTCTCTGCAAGTCACGTCATCGAAACAGGAAGCCTGCCCTCGTGAGAGCAGGATTCCCCGCGCCACCGTGCGCGGGGTAACATTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1242 bp | 413 aa | 105 | 1346 | + | No |
AG : TnpB
ORF sequence :
MEYSHRYRAYPTQEVAERLEHHLDVHRQLYNHVRWDYQNSPQDDKPSEYDQNNKLPEWKQKWSLFGELHSKAAQATVARFHHNLTVLSELKEKGYTVGRL
KRQAPSEYRSVTYNQSGFDLDEKRGHDNYAYVRFSKIGWVKIRYSRPIPDHATIKEVSFKKETTGEWFVSFGLETDEAGLPEKPDGDSLDASNSVGIDLG
IQNYVHTSDGKTVDWLDLEDDYERLRREQRKLSRKEKGSNNYEKHRREVAKVKRHIKRKVLDYQHKSTTWLVKEYDAVFVEDLNVAGMLQEDGNARNKQD
AAWRQFITLLDYKADLYGTHVVEVEARGTTKECASCGVETAKPIWIREHSCPSCGFETDRDANAAMNVLKRGFAELGLGWPEETPVETVTATDADDFREV
SASHVIETGSLPS
KRQAPSEYRSVTYNQSGFDLDEKRGHDNYAYVRFSKIGWVKIRYSRPIPDHATIKEVSFKKETTGEWFVSFGLETDEAGLPEKPDGDSLDASNSVGIDLG
IQNYVHTSDGKTVDWLDLEDDYERLRREQRKLSRKEKGSNNYEKHRREVAKVKRHIKRKVLDYQHKSTTWLVKEYDAVFVEDLNVAGMLQEDGNARNKQD
AAWRQFITLLDYKADLYGTHVVEVEARGTTKECASCGVETAKPIWIREHSCPSCGFETDRDANAAMNVLKRGFAELGLGWPEETPVETVTATDADDFREV
SASHVIETGSLPS
Blast result :
Comments
ISNaoc7 is 94% (TnpB) aa similar to ISNph11.
References
1] Pfeiffer, F. (2015) Direct submission
2] Eisen,J., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J., Klenk,H.-P. and Klenk,H.-P. (2012) Direct submission GenBank.
2] Eisen,J., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J., Klenk,H.-P. and Klenk,H.-P. (2012) Direct submission GenBank.