ISfinder Mirror Site

ISHla22

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001367	ND	Halorubrum lacusprofundi	Halorubrum lacusprofundi ATCC 49239 plasmid pHLAC01

DNA section

IS Length : 1515 bp

Ends

Left end : CTGAACTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAGGGAGCGAGTAGGGAGGGGCCTTAGCGCCGCACGAGTAACAAACACGTTTGACGCTC II struct. : Yes
Right end : CCCTGTGGATACCTCGGTATCTGCAAAGCGCGTCGTGGAAACAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CTGGCTTCCAGT	TTAC	GGTGGAATCGAA	tcac

DNA sequence

CTGAACTGAGGCGCTAAGGCCCCTTCCTCAACGAGCGACCGAAGGGAGCGAGTAGGGAGGGGCCTTAGCGCCGCACGAGTAACAAACACGTTTGACGCTC
GGCCCACAGGCCCACGCAACCGCAACAACCATACGTATAAGTGCGCACAATGCGTACATAGTGAGACGGAAAAACATCACCATCCGCGAAGACCAAGCCG
AGTGGATCGAGGAGAACCACCTCAACCTCTCGTCGTTCGTTCGCGGACAACTTGACGAACTCATCGAAGAACGCTCGTGAACTACAACTACAGGTATCGA
CTCCGACCGTCCGACGCTCTCGAACAACAGTTAGCGTGGACTGTCGATACCTGTAGACAGGTATACAACCACTTCCTCCACCGGCTCAACCGCACTGACG
ACACCTCGGCATACAGCGAGCAGAAACTCCTGCCGAGCCTCAAGAAGTGGTGGAACGACCTGAAACACGTTCACTCGAAAGTCCTTCAGAAAGTCGTACA
ACGGCTGTACGACAACCTCTCAACGCTTCGTGGTCGTAAAGAGAATGGCTACCACGTCGGGACGCTCAAGTGGAAGGCACCGAGCGAGTACCGCAGTTTC
ACCTACGGTCAATCCGGCTTCAAGCTCAAGAACACGAGCGGTCGGACTCGGCTGTGGCTCTCGAAGCTCGGAGAAATCCCGCTCACCTTCCACCGCGCCC
TCCCCGACGACGCCGAAATCAAGACGGTCACAGTCAAACAGGAACCGACCGGGAAGTGGTACGCCATCCTCGGCGTCGAAACCCCGGACGACCCGCCCGC
GAAACCGGAGACTCCCGAGAAGTGCGTTGGCATCGACGTAGGGATTCTCAAGTACGCCCACGACACCGACGGAACAGCTGTCGAATCGTTCGATCTATCC
GATGAACGTGAGCGGTTGGAACGCGCCCAACGCGACCTCTCGCGGAAGGAACACGGTTCCACAAATTGGGAGAAACAGCGCCGTGTCGTGGCCGAGCGTC
ACGCCGAGTTGAAGAACAAGCGACGGGACTTCCTCCACAAGCTCTCGAACTACTACGCCCGCGAATACGACTTCGTGGCCGTGGAGGACTTGGACGTAAA
GGGCTTGATCGAACTGCCTGGCAACTCGCGGAACCGAGCGGGAGCGGCGTGGGGAACGTTCCTACGGATGCTCGAATACAAGTGCGAACGGGAAGGAACG
TATTTTATCGCGGTGAATCCTCGTGGGACGACGAAAGAGTGTGCGTCCTGCGACGCTTCGACGGAGAAGCCGCTGTGGGTGCGGGAGCATTCCTGTCCGG
CGTGTGGTTTCGAGGCGGATAGAGACGCGAACGCGGCGTGGAACATTCTTTCTCGCGGTATCACAAAGCGGTTAGGAGCGGGACGCTCCGAATCAACGCC
TGTGGAGACTGCGCTCCCTGTGGATACCTCGGTATCTGCAAAGCGCGTCGTGGAAACAGGAAGCCCCACCCTCAAGCGCGAGCCGTCAGGCGAGCGGTAG
GGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
120 bp	39 aa	161	280	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MRRKNITIREDQAEWIEENHLNLSSFVRGQLDELIEERS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1224 bp	407 aa	277	1500	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MNYNYRYRLRPSDALEQQLAWTVDTCRQVYNHFLHRLNRTDDTSAYSEQKLLPSLKKWWNDLKHVHSKVLQKVVQRLYDNLSTLRGRKENGYHVGTLKWK
APSEYRSFTYGQSGFKLKNTSGRTRLWLSKLGEIPLTFHRALPDDAEIKTVTVKQEPTGKWYAILGVETPDDPPAKPETPEKCVGIDVGILKYAHDTDGT
AVESFDLSDERERLERAQRDLSRKEHGSTNWEKQRRVVAERHAELKNKRRDFLHKLSNYYAREYDFVAVEDLDVKGLIELPGNSRNRAGAAWGTFLRMLE
YKCEREGTYFIAVNPRGTTKECASCDASTEKPLWVREHSCPACGFEADRDANAAWNILSRGITKRLGAGRSESTPVETALPVDTSVSAKRVVETGSPTLK
REPSGER

Blast result :

Comments

ISHla22 is 89% (TnpB) aa similar to ISNma17. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.