ISfinder Mirror Site

ISMno21

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001350	ND	Methylobacterium nodulans	Methylobacterium nodulans ORS 2060 plasmid pMNOD01

DNA section

IS Length : 1162 bp

Ends

IR Length : 18/24

IRL : CCAAGCCTCGTTGATCGAGACTCACGCAGGCTGGCGGTGGGGTCCTCAAC
IRR : CCAACTCTCATTGATCAGAACCCATGGGCCCAGGCGCGCAAGCCGATGGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCTCTTCTAA	TA	TAAGTGTCTC	2
TCCTCAACTA	TA	TTAGCCTCAT	2
TTGGCCTTCA	TA	TCCCAACCCG	2

DNA sequence

CCAAGCCTCGTTGATCGAGACTCACGCAGGCTGGCGGTGGGGTCCTCAACTATGATTCTGTCCTCGGGTTTGAAGCGCGAGGAGGCTGGATTGGCCGCTC
CCGTTCCGCTGCGGTCGGACTTTGACGCCGCGGCTCTGCGTCGTTTGGCCAGAGCGTCGCGCGATCCGGCTCAAACCCGCCGTCTGCTGGCGCTGTCGGC
GATCGACGCGGGCGGGTCACGCACGCAGGCCGCCGCGCTCGGCGGGGTCGGGCTGCAGACGGGGCGCGACTGGGTGCTGGCCTTCAACGCAGACGGCCCG
GACGGGCTGATCGACGCCAAGGCCCCCGGTGCGCGCCCGCGCCTGAACGCCCGGCAGCGGGAGGCGCTGCGCGCCTTGGTCGAGCAGGAGCCCATGCCCG
CCATCCATGGTGTCGTGCGCTGGCGGCTGGTCGATCTGGTCCAGATCCTGTTCGAGGACCACGGCGTCTCGGTCTCCCAACAGACGCTGAGCCGGGTTCA
GCGCGGCATGGGCTATCGGAAGCTCTCGGCCCGTCCCCGCCATCACGCCCAGGATCCGCAGGCCACCGCCGCGTTTAAAAAGCCTTCCCCGCCCGCTGGG
CGCAGATCGCGCAGGCCACAGGCGGCAAGCCGATAGAGATCTGGTTTGCCGACGAGGCCCGCGTCGGCCAGACGAACACCATCCCGCGGCGCTGGGCGCG
CCGCGGCACACGCCCCTCGGCTCCGAAGGACCAGCGCACGGCCTCCGCCTCCCTGTTCGGGGCGATCTGCCCGGCCCGTGGCACGGGAGCCGGGCTCGTC
ATGCCCCGCTGCACCACCGAAGCGATGAACCTGCACCTGGCTGAGATCGCCAAGGCCGTCGCGCCGGGCGCGCACGCCGTCCGGCTGCTCGATCAGGCCG
GCTGGCACACGACCAGACAACTCGTGGTCCCTGCCAACATCACCCTGCTGCCGCTGCCAGCGCGCTCGCCTGAACTCAACCCAGTGGAGAACATCTGGCA
GTTTATACGCGAGAACTGGCTCGGCAACCGGATCTTCGCCTCCTCCGACGCCATCCTCGATCACTGCTGCCATGCCTGGAACAGGCTCATCGACCAGCCC
TGGCGCATCATCTCCATCGGCTTGCGCGCCTGGGCCCATGGGTTCTGATCAATGAGAGTTGG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
585 bp	194 aa	52	636	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MILSSGLKREEAGLAAPVPLRSDFDAAALRRLARASRDPAQTRRLLALSAIDAGGSRTQAAALGGVGLQTGRDWVLAFNADGPDGLIDAKAPGARPRLNA
RQREALRALVEQEPMPAIHGVVRWRLVDLVQILFEDHGVSVSQQTLSRVQRGMGYRKLSARPRHHAQDPQATAAFKKPSPPAGRRSRRPQAASR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
570 bp	189 aa	579	1148	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KAFPARWAQIAQATGGKPIEIWFADEARVGQTNTIPRRWARRGTRPSAPKDQRTASASLFGAICPARGTGAGLVMPRCTTEAMNLHLAEIAKAVAPGAHA
VRLLDQAGWHTTRQLVVPANITLLPLPARSPELNPVENIWQFIRENWLGNRIFASSDAILDHCCHAWNRLIDQPWRIISIGLRAWAHGF

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1097 bp	365 aa	52	1148	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MILSSGLKREEAGLAAPVPLRSDFDAAALRRLARASRDPAQTRRLLALSAIDAGGSRTQAAALGGVGLQTGRDWVLAFNADGPDGLIDAKAPGARPRLNA
RQREALRALVEQEPMPAIHGVVRWRLVDLVQILFEDHGVSVSQQTLSRVQRGMGYRKLSARPRHHAQDPQATAAFKKAFPARWAQIAQATGGKPIEIWFA
DEARVGQTNTIPRRWARRGTRPSAPKDQRTASASLFGAICPARGTGAGLVMPRCTTEAMNLHLAEIAKAVAPGAHAVRLLDQAGWHTTRQLVVPANITLL
PLPARSPELNPVENIWQFIRENWLGNRIFASSDAILDHCCHAWNRLIDQPWRIISIGLRAWAHGF

Blast result :

Comments

ISMno21 is 71% aa similar to ISGdi4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.