ISMdi15

  • Family IS200/IS605
  • Group IS1341
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_012811 ND Methylobacterium extorquens
Methylobacterium extorquens AM1
Methylobacterium dichloromethanicum DM4
DNA section
IS Length : 1344 bp

Ends


Left end : TGCTCCCGCCAGACCGTGCAGTGGTGGGCGGGGGGTGAAGCCGCCAGCGCGAAGCGCTTGGCCGCTTTCGTACACGCTCGGCGTAAGGTATGTTCCCGTT II struct. : Yes
Right end : CGGCGTGGGTGCTCTGGAACATCGGTCAGGAACTGGCCGCGGGTGCGAGCCCGGAAACCGCGCTCGGAGCCGCGATAGCGGCTTGAGGGCGGTAGTTCAT II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
AAAACCGCAGCTAGTTCCGCCATAtcat

DNA sequence

TGCTCCCGCCAGACCGTGCAGTGGTGGGCGGGGGGTGAAGCCGCCAGCGCGAAGCGCTTGGCCGCTTTCGTACACGCTCGGCGTAAGGTATGTTCCCGTT
ATGTACCCCGTCCACCGCCGCGTGACGTACAAGCTGTACCCGTCGCCCGTTCAGGCGGCGGCGCTGGAGCGCGTCTGCGACCTGCACCGGGCGCTGTACA
ACGCGGCCCTGGAGGAGCGGATCGAGGCGTATCGCAAGGCGGGCAGGTCGATCGGCTTTGCCGACCAGTGCATATCGGTCACCGCGATCCGGCTGGACGA
CCCAGCCTATCGGGTGCTCAACGCGCAGTCTCTTCAGGTCACGCTCAAGCGGCTGGATCGGGCCTTCCAGAGCTTCTTCCGGCGCGTGAAGGCCGGGCAG
GCGCCTGGGTTCCCGCGCTTCAAGGGTCGCGACCGCTTCCCCGGCTTCGGCTTCAAGAGCCACGGCGACGGCTTCCGCTTCACGCCCGGCCAGGGCTGGC
GGAACGGGACGCTGCACCTCTCCGGCATCGGAGAGATGACGGCCCGCGGCGAAGCGCGCACGCCCGGCGAGCTCGTCTGCGCGGACGTGCAGCGCAGGGC
GGAGGGCTGGTTCCTGTCCCTGGTGGTCTCGTGCGAGCCGCATCGGGAACGCATGGGCGAAAGCATCGCCGGGCTGGATTGGGGCGTGGAGACCTATGCC
ACGCTCTGCACCGGGCCGATGCAGTTCGGTGAGATCGAGAACGAGCGCTTCCTCGCGGTCGAGCAGGAGGCGCTGAAGGAGGAGCAGCGCGCCCTCTCGC
GGGCGCTGCGCGGCAAGCGCTCGAAGCGCGCCGCCAAGGCGCGCAAGCTCATCGCCCGGCGCTCGCGCCACCTCGCGAACCGACGCAAGAACCGGACCCA
TCAGGTCACCGCCCGGCTCGTGCGCGGGCACGCGACGATCGTCACGGAGGACCTGTCCGTCCGCAACATGACCGCCTCGGCCAGGGGCAGCGTCGAAGAG
CCGGGTCGTCGCGTTAAGCAGAAGGCCGGGCTGAACCGGGCCATCCTCGACACCGCGCCTGGATCGTTTCTCTCCCACCTGCGCATCAAAGCGGAAGAAG
CTGGATGCGTGTTGGTTCTCGTCGATCCTCGCAGGCACAGGCCGTCGCAGACCTGTCCCGTCTCTGGCGAGGTTCGGAAGGAGGGCTTGGGCGAGCGCAC
CCACACCCTGCCGGACGGGCGAGTGATCTCGCGCGATCAGGCCTCGGCGTGGGTGCTCTGGAACATCGGTCAGGAACTGGCCGCGGGTGCGAGCCCGGAA
ACCGCGCTCGGAGCCGCGATAGCGGCTTGAGGGCGGTAGTTCAT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1231 bp409 aa1011331+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MYPVHRRVTYKLYPSPVQAAALERVCDLHRALYNAALEERIEAYRKAGRSIGFADQCISVTAIRLDDPAYRVLNAQSLQVTLKRLDRAFQSFFRRVKAGQ
APGFPRFKGRDRFPGFGFKSHGDGFRFTPGQGWRNGTLHLSGIGEMTARGEARTPGELVCADVQRRAEGWFLSLVVSCEPHRERMGESIAGLDWGVETYA
TLCTGPMQFGEIENERFLAVEQEALKEEQRALSRALRGKRSKRAAKARKLIARRSRHLANRRKNRTHQVTARLVRGHATIVTEDLSVRNMTASARGSVEE
PGRRVKQKAGLNRAILDTAPGSFLSHLRIKAEEAGCVLVLVDPRRHRPSQTCPVSGEVRKEGLGERTHTLPDGRVISRDQASAWVLWNIGQELAAGASPE
TALGAAIAA

 

Blast result :
Comments
ISMdi15 is 52% (TnpB) aa similar to ISCARN6.
The original copy in Methylobacterium dichloromethanicum DM4 is mutated (unexpected frameshifts in TnpB), it was replaced by a complete copy in Methylobacterium extorquens AM1.
References
1] Stephane Vuilleumier et.al. (2009) PLoS ONE 4 (5), E5584