ISVpa4
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KP688397 | Y | Vibrio parahaemolyticus | Klebsiella pneumoniae Vibrio parahaemolyticus V36 plasmid pVPH1 |
DNA section
IS Length : 1260 bp
Ends
IR Length : 20/25
IRL : AGAAGTGGCCCCCAATTTTCGGACATGACTTTAAGTGGTCGATCTGTTTT
IRR : AGAAGTGGACCCCAACGGTTGGACACATAAGCTCATTTCTTAAGTGGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATTGAGAAG | TAACACTTTC | 0 |
DNA sequence
AGAAGTGGCCCCCAATTTTCGGACATGACTTTAAGTGGTCGATCTGTTTTGATAGTACCCAACAATACAGGGACAGATTATGACTAGAAAACGTAGAAAC
CACTCTCCTGAGTTTAAAGCTAAGGTGGCTCTCGATGCCGCTAAAGGCGATAAAACCGTCGCTGAGCTAGCTCAGAAATATAACCTGCACGCTAACCAGA
TCTCAACATGGAAAAAGGAGCTGCTTGAAAACGCAGCCATGATTTTTGCCACCGAAAATCACTCAGGAAAAGAGAGTTCCGAAGAAGTGGACAAACTTCA
CGCCAAGATCGGTCAATTGACCATGGAAAATGATTTTTTGGCCAAAGTGCTCGGTCGTTAGACCGAGCCCAGCGAAAGAGTTCGCTGGTTAAATCCACCC
CATTGCCGATAAAGCGCCAATGTGAGCTGCTCAATATTGCTCGCTCTACCGCTTACTATCAACCCATCGGACTCTCTGCTGAGGAGATCACGTTACGACG
AATGATTGACGAAATTCATCTGCAGTATCCGTTTATGGGCAGTCGGCGCATTCGAACTGAGCTGGCTAAAAATGGCCATAGCGTTAATCGTAAGCGTGTT
GTTCGACTCATGCGCGATATGGGGATTGGGGCGATTTACCCCAAGCCCAAAACGACGCTGGCGAACAAAGCACACAAGGTGTATCCCTACCTGTTGCGTG
ATATCGAAGTCACTTACCCAAACCAAGCTTGGGCGATTGATATCACATATATCCCAATGGCGAAGGGGTTCCTGTACCTCGTTGCGATTATCGACTGGTA
TAGCCGCAAAGTGCTGGCTTGGCGACTATCCAACACCATGGACACGAGTTTTTGTATCGAAGCGCTTGAGGAAGCGCTGAAGCATTATGGGCCACCTGAT
ATCTTTAACTCAGATCAAGGCAGCCAGTTTACCAGCACAGAGTTCACACAGAAGTTAATTGAGCATAACGTACGTATAAGCATGGATGGGAAAGGCCGCT
GGGTTGACAATGTTTTCATTGAGCGATTATGGCGAAGCCTGAAATATGAGGAGGTTTACTTAAAGGCTTATACCACGCCCCGTGAAGCCGAGCTTGAAAT
CGGCAACTACATGGTGTTTTATAATGAAGAGCGTAACCATCAAGGACTCAATAACCTCACTCCTGATGAGGCCTACTTCGGTAGGCAAAGATACGCAGCA
TGAGCTGGGTCAACCACTTAAGAAATGAGCTTATGTGTCCAACCGTTGGGGTCCACTTCT
CACTCTCCTGAGTTTAAAGCTAAGGTGGCTCTCGATGCCGCTAAAGGCGATAAAACCGTCGCTGAGCTAGCTCAGAAATATAACCTGCACGCTAACCAGA
TCTCAACATGGAAAAAGGAGCTGCTTGAAAACGCAGCCATGATTTTTGCCACCGAAAATCACTCAGGAAAAGAGAGTTCCGAAGAAGTGGACAAACTTCA
CGCCAAGATCGGTCAATTGACCATGGAAAATGATTTTTTGGCCAAAGTGCTCGGTCGTTAGACCGAGCCCAGCGAAAGAGTTCGCTGGTTAAATCCACCC
CATTGCCGATAAAGCGCCAATGTGAGCTGCTCAATATTGCTCGCTCTACCGCTTACTATCAACCCATCGGACTCTCTGCTGAGGAGATCACGTTACGACG
AATGATTGACGAAATTCATCTGCAGTATCCGTTTATGGGCAGTCGGCGCATTCGAACTGAGCTGGCTAAAAATGGCCATAGCGTTAATCGTAAGCGTGTT
GTTCGACTCATGCGCGATATGGGGATTGGGGCGATTTACCCCAAGCCCAAAACGACGCTGGCGAACAAAGCACACAAGGTGTATCCCTACCTGTTGCGTG
ATATCGAAGTCACTTACCCAAACCAAGCTTGGGCGATTGATATCACATATATCCCAATGGCGAAGGGGTTCCTGTACCTCGTTGCGATTATCGACTGGTA
TAGCCGCAAAGTGCTGGCTTGGCGACTATCCAACACCATGGACACGAGTTTTTGTATCGAAGCGCTTGAGGAAGCGCTGAAGCATTATGGGCCACCTGAT
ATCTTTAACTCAGATCAAGGCAGCCAGTTTACCAGCACAGAGTTCACACAGAAGTTAATTGAGCATAACGTACGTATAAGCATGGATGGGAAAGGCCGCT
GGGTTGACAATGTTTTCATTGAGCGATTATGGCGAAGCCTGAAATATGAGGAGGTTTACTTAAAGGCTTATACCACGCCCCGTGAAGCCGAGCTTGAAAT
CGGCAACTACATGGTGTTTTATAATGAAGAGCGTAACCATCAAGGACTCAATAACCTCACTCCTGATGAGGCCTACTTCGGTAGGCAAAGATACGCAGCA
TGAGCTGGGTCAACCACTTAAGAAATGAGCTTATGTGTCCAACCGTTGGGGTCCACTTCT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 80 | 361 | + | No |
Description : First part of the transposase
ORF sequence :
MTRKRRNHSPEFKAKVALDAAKGDKTVAELAQKYNLHANQISTWKKELLENAAMIFATENHSGKESSEEVDKLHAKIGQLTMENDFLAKVLGR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 870 bp | 289 aa | 334 | 1203 | + | No |
Description : Second part of the transposase
ORF sequence :
FFGQSARSLDRAQRKSSLVKSTPLPIKRQCELLNIARSTAYYQPIGLSAEEITLRRMIDEIHLQYPFMGSRRIRTELAKNGHSVNRKRVVRLMRDMGIGA
IYPKPKTTLANKAHKVYPYLLRDIEVTYPNQAWAIDITYIPMAKGFLYLVAIIDWYSRKVLAWRLSNTMDTSFCIEALEEALKHYGPPDIFNSDQGSQFT
STEFTQKLIEHNVRISMDGKGRWVDNVFIERLWRSLKYEEVYLKAYTTPREAELEIGNYMVFYNEERNHQGLNNLTPDEAYFGRQRYAA
IYPKPKTTLANKAHKVYPYLLRDIEVTYPNQAWAIDITYIPMAKGFLYLVAIIDWYSRKVLAWRLSNTMDTSFCIEALEEALKHYGPPDIFNSDQGSQFT
STEFTQKLIEHNVRISMDGKGRWVDNVFIERLWRSLKYEEVYLKAYTTPREAELEIGNYMVFYNEERNHQGLNNLTPDEAYFGRQRYAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1124 bp | 374 aa | 80 | 1203 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRKRRNHSPEFKAKVALDAAKGDKTVAELAQKYNLHANQISTWKKELLENAAMIFATENHSGKESSEEVDKLHAKIGQLTMENDFFGQSARSLDRAQRK
SSLVKSTPLPIKRQCELLNIARSTAYYQPIGLSAEEITLRRMIDEIHLQYPFMGSRRIRTELAKNGHSVNRKRVVRLMRDMGIGAIYPKPKTTLANKAHK
VYPYLLRDIEVTYPNQAWAIDITYIPMAKGFLYLVAIIDWYSRKVLAWRLSNTMDTSFCIEALEEALKHYGPPDIFNSDQGSQFTSTEFTQKLIEHNVRI
SMDGKGRWVDNVFIERLWRSLKYEEVYLKAYTTPREAELEIGNYMVFYNEERNHQGLNNLTPDEAYFGRQRYAA
SSLVKSTPLPIKRQCELLNIARSTAYYQPIGLSAEEITLRRMIDEIHLQYPFMGSRRIRTELAKNGHSVNRKRVVRLMRDMGIGAIYPKPKTTLANKAHK
VYPYLLRDIEVTYPNQAWAIDITYIPMAKGFLYLVAIIDWYSRKVLAWRLSNTMDTSFCIEALEEALKHYGPPDIFNSDQGSQFTSTEFTQKLIEHNVRI
SMDGKGRWVDNVFIERLWRSLKYEEVYLKAYTTPREAELEIGNYMVFYNEERNHQGLNNLTPDEAYFGRQRYAA
Blast result :
Comments
ISVpa4 is 74% aa similar to ISAzo18.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This IS has atypic IRs: it begins with 'AG' instead of 'TG'.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
This IS has atypic IRs: it begins with 'AG' instead of 'TG'.
References
1] Papagiannitsis C.C. (2015) Direct submission