ISUnCu17
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KC964606 | ND | Uncultured bacterium | Uncultured bacterium plasmid pMLUA3 |
DNA section
IS Length : 1481 bp
Ends
IR Length : 13/14
IRL : AGCCTCTTGCAAAAGGCGCACTGCGTGGGGGGTTTTTTGCGGCGGGAGGC
IRR : AGCTTCTTGCAAAACTCTAGGTTGAAGAATGCTGCGCCGGCGCACTTGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTTTATTTA | CTCCTG | TTTAGCTCTT | 6 |
DNA sequence
AGCCTCTTGCAAAAGGCGCACTGCGTGGGGGGTTTTTTGCGGCGGGAGGCAAAGGCGGAGGGTGCGGGCGGCCATTTCTGGCATGATGCGGTTACCAAGC
CAAACATCATAACGCCCGCGCCATGAATGCTACTCAACGCGAACTGATCCTGCAACGCTGGAACGTGATTCAACACGAACTGCTGCCCGAGTTGAAGAAC
GAGGTGGGCGCGCTGACGCCGAAGCTGGAGCGCGTCATCCACACCCTGGAATGGGTGCGCATCGAGGAATTCACGGCGACGACGTGGTGTGGCGTGGGTC
GTCCGCCCTGTGAGCGGGCTTGGCTGGCGAACGCTTTCGTGGCGAAGGCGGTGCTGGGACTGACGACCACGGTGGGGCTGATCGAGCGCCTGACGGTGGA
TCGTGCGCTGCGGCGCATCTGTGGCTTCCCGCTGTGCCGGAAACTGCCCTCCGAAGCCACCTTCTCGCGCGCGTTTGACGAGTTTGCTGAGGGGCGTCTG
GCCGAACGGGTGCATGAGGCGCTCGTCAAGGAACACCTCGGGGATGAACTGATCGGCCACATCAGCCGCGACGGCACCGCGATCGAAGCGCGCGAGCGCC
CGGCCCACGCCAAGGGTGCGGCGGCGACACCGGCGCCTGCGGCCCAATCGACCCTGATCGCCGAGGAAGAAACCCGCGCGCCAACGCCCCCGGTCCTGGC
GAAGAAACGCGGCCGCCCGCGGCGCGGCGAAGTCCGTGCACCCGCCAAGGTGTCGCCCCTCCAGCGCCAACGCCAGCAGAGCCTGGCGCAGATGCTCAAG
GACATCCCCACGGCGTGCGACCGCGGCACCAAGTGCAATGCCCAAGGGTACAAAGTCAGCTGGAATGGCTACAAGCTGCACCTGGACACCGCCGACTGTG
GTGTGCCGATTGCAGCGCTGCTCTCGTCCGCGTCGATGCACGACAGCCGCGCCGCCATTCCTTTGTCGCTGATCAGCGCGCAGCGCGTCACCAATCTCTA
CGACGTCATGGATGCCGCCTATTGCAGCTTCGAACTGCATAAGCACTGCCGTAGCCTCGGCCATGTCCCTTTGATCGACCACAATCCGCGCGGCGGCCTG
AAGGAGGCGTTCGAGCCTGCCGACGCGATCCGCTACAACGAACGCACCGTCGCCGAGCGCAGCAATGCGCGGCTCAAGGATGAGTTCGGCGGTAACACCC
TCCGGGTCAAGGGCAGCACCAAGGTCATGGGGCACCTGATGTTCGGTGTCCTGGCCCTGTCTGCCGATCAGTTGATGCGATTGCGACAATGAAAACGCTC
CGCCTGCATTCTCACCGGACGATGCATGCACAGATGCAGGGAGCGCCTCGCGCCGGAACATCGGAAAATGATGATTAGTTAAGCCGGAAGTCGGATCGCA
CCAGAGCCGCCGTCAAAAAACGCGTGAAAACCGCAAGTGCGCCGGCGCAGCATTCTTCAACCTAGAGTTTTGCAAGAAGCT
CAAACATCATAACGCCCGCGCCATGAATGCTACTCAACGCGAACTGATCCTGCAACGCTGGAACGTGATTCAACACGAACTGCTGCCCGAGTTGAAGAAC
GAGGTGGGCGCGCTGACGCCGAAGCTGGAGCGCGTCATCCACACCCTGGAATGGGTGCGCATCGAGGAATTCACGGCGACGACGTGGTGTGGCGTGGGTC
GTCCGCCCTGTGAGCGGGCTTGGCTGGCGAACGCTTTCGTGGCGAAGGCGGTGCTGGGACTGACGACCACGGTGGGGCTGATCGAGCGCCTGACGGTGGA
TCGTGCGCTGCGGCGCATCTGTGGCTTCCCGCTGTGCCGGAAACTGCCCTCCGAAGCCACCTTCTCGCGCGCGTTTGACGAGTTTGCTGAGGGGCGTCTG
GCCGAACGGGTGCATGAGGCGCTCGTCAAGGAACACCTCGGGGATGAACTGATCGGCCACATCAGCCGCGACGGCACCGCGATCGAAGCGCGCGAGCGCC
CGGCCCACGCCAAGGGTGCGGCGGCGACACCGGCGCCTGCGGCCCAATCGACCCTGATCGCCGAGGAAGAAACCCGCGCGCCAACGCCCCCGGTCCTGGC
GAAGAAACGCGGCCGCCCGCGGCGCGGCGAAGTCCGTGCACCCGCCAAGGTGTCGCCCCTCCAGCGCCAACGCCAGCAGAGCCTGGCGCAGATGCTCAAG
GACATCCCCACGGCGTGCGACCGCGGCACCAAGTGCAATGCCCAAGGGTACAAAGTCAGCTGGAATGGCTACAAGCTGCACCTGGACACCGCCGACTGTG
GTGTGCCGATTGCAGCGCTGCTCTCGTCCGCGTCGATGCACGACAGCCGCGCCGCCATTCCTTTGTCGCTGATCAGCGCGCAGCGCGTCACCAATCTCTA
CGACGTCATGGATGCCGCCTATTGCAGCTTCGAACTGCATAAGCACTGCCGTAGCCTCGGCCATGTCCCTTTGATCGACCACAATCCGCGCGGCGGCCTG
AAGGAGGCGTTCGAGCCTGCCGACGCGATCCGCTACAACGAACGCACCGTCGCCGAGCGCAGCAATGCGCGGCTCAAGGATGAGTTCGGCGGTAACACCC
TCCGGGTCAAGGGCAGCACCAAGGTCATGGGGCACCTGATGTTCGGTGTCCTGGCCCTGTCTGCCGATCAGTTGATGCGATTGCGACAATGAAAACGCTC
CGCCTGCATTCTCACCGGACGATGCATGCACAGATGCAGGGAGCGCCTCGCGCCGGAACATCGGAAAATGATGATTAGTTAAGCCGGAAGTCGGATCGCA
CCAGAGCCGCCGTCAAAAAACGCGTGAAAACCGCAAGTGCGCCGGCGCAGCATTCTTCAACCTAGAGTTTTGCAAGAAGCT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1359 bp | 389 aa | 123 | 1481 | + | No |
Chemistry : DDE
ORF sequence :
MNATQRELILQRWNVIQHELLPELKNEVGALTPKLERVIHTLEWVRIEEFTATTWCGVGRPPCERAWLANAFVAKAVLGLTTTVGLIERLTVDRALRRIC
GFPLCRKLPSEATFSRAFDEFAEGRLAERVHEALVKEHLGDELIGHISRDGTAIEARERPAHAKGAAATPAPAAQSTLIAEEETRAPTPPVLAKKRGRPR
RGEVRAPAKVSPLQRQRQQSLAQMLKDIPTACDRGTKCNAQGYKVSWNGYKLHLDTADCGVPIAALLSSASMHDSRAAIPLSLISAQRVTNLYDVMDAAY
CSFELHKHCRSLGHVPLIDHNPRGGLKEAFEPADAIRYNERTVAERSNARLKDEFGGNTLRVKGSTKVMGHLMFGVLALSADQLMRLRQ
GFPLCRKLPSEATFSRAFDEFAEGRLAERVHEALVKEHLGDELIGHISRDGTAIEARERPAHAKGAAATPAPAAQSTLIAEEETRAPTPPVLAKKRGRPR
RGEVRAPAKVSPLQRQRQQSLAQMLKDIPTACDRGTKCNAQGYKVSWNGYKLHLDTADCGVPIAALLSSASMHDSRAAIPLSLISAQRVTNLYDVMDAAY
CSFELHKHCRSLGHVPLIDHNPRGGLKEAFEPADAIRYNERTVAERSNARLKDEFGGNTLRVKGSTKVMGHLMFGVLALSADQLMRLRQ
Blast result :
Comments
ISUnCu17 is 94% aa similar to ISAzo25.
ISUnCu17 was found in the IncP-1epsilon plasmid pMLUA3, captured by exogenous isolation from estuarine waters.
ISUnCu17 was found in the IncP-1epsilon plasmid pMLUA3, captured by exogenous isolation from estuarine waters.
References
1] Oliveira C.S., Moura A., Henriques I., Brown C.J., Rogers L.M., Top E.M. and Correia A. (2013) Plasmid 70 (3), 412-419