ISfinder Mirror Site

ISHla21

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP001366	ND	Halorubrum lacusprofundi	Halorubrum lacusprofundi ATCC 49239 plasmid pHLAC01 Halorubrum lacusprofundi ATCC 49239

DNA section

IS Length : 1835 bp

Ends

Left end : AGAGTCACGAGCGCATACCCCCGTCTTTAGGCGGGGGTCAAGCGGACAATAGCGTACATCCCCACCGACGACGCTATGGCTGGATTTCCCACCGATTCAC II struct. : No
Right end : AGAGACGCTTGGAACGTATGCGTCTTGAAACCGTAGGGCCACGCTCGGCCTGAAATCCCGTGGTGGGATTCCCGCGTCTTCAGGCGCGGGAGGAGGTCAA II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CTCTGTATCGAC	TGAT	TTGCAGAGTGGG
AGTTTCGGAAGG	TGAT	TGCGGACACACC

DNA sequence

AGAGTCACGAGCGCATACCCCCGTCTTTAGGCGGGGGTCAAGCGGACAATAGCGTACATCCCCACCGACGACGCTATGGCTGGATTTCCCACCGATTCAC
CATCAGTATTAATATACATTGTCTCATAGTGTACAACACGGATGAAGACCACACGGCACGCGACCTACAACCTCAACTACCACCTTGTGTGGTTGCCGAA
GTACCGCAACTCGGTACTCGTCAACGAGGTCGCAGACCGTGTGCGGACCATCCTCCACGAAATAGCCGACGACAAGGGCCTCGAAATACTTGACCTGACC
GTACAACCCGATCACGTCCACCTATTCGTCAGTAGCCCACCGAAACACGCACCATCCCTTCTCGCCAACTGGTTCAAAGGTATCAGCTCTCGGAAGTACA
ACCACCGCTACGCCGACCACGACGGCGAGAAAATCGGATGGGTGAGAGGCTACTACGCAGGGACAGCAGGGCACGTATCGAGCGAGACGGTCAAGAACTA
CATCCAGCGTCACGAGGAGGACGAGTCGTGACCACGCTCACGAAGACGCTGGAACTGAAACTGGTAGAGCCGAACGCGCACAAGCGGAGAAAACTCCGCG
AGACGCGAGAGGCGTACCAACAGGCGCTTCACGACGCCTTCGACGCTGGCTGTACCACACAGACCGAAGCGAACGACGTGGTGGTCAACTACAAGCTGAG
TGGATACGCAAAGAATGCGCTCAAGAAGTACGTCCCGCAACTCACGACGACGTACAACGCAGGCGAACTTCACGATGCCCACCCCGTCCGTTTCACGAAC
GAAGGGCTACGACTCGACCACAAGCCCGAGAACGCTATCGAGTGGTACGTCAAAATCCCGCACCACGAGGACTACCACCTCTGGATGCCAGCACAACCGA
ATCCCGAACAACGGGACTGGTTGGAAGCGTTGAACGCGGGAGACGCCACGATGGGCGAGAGTCGGCTATTCGAGCGGGACGGAACGTGGTATCTTCACGT
CACCGCAACCCGCGACGCAGAGGGCGGTTCCGAGGTGTCCGACGAAGAACGGACACCCATCGGTGTCGATATTGGGGAAGCGTCGCTCGTCACGGTGTGT
CACCGTGACGACCACGGTTGCCCGACCGCTCCCGAACTATGGGCCGACGAAGGGAAGACCGTTCGTCGGCTCCGCAAGACCTACTTCACCGCCACGAGGC
GACTTCAGACACGCGGTAGCGAACGTATCGCGGAGTCCTTCGGTGACGACCTGTGGAACCAGATAGACGACGTGTTCCACCGCGTCACCCGCGAAGTCGT
GGACTACGCCGAGTCTGTCAAGAATCCCGTTCTTGTTCTGGAAGACCTGACGTACATACGGGAGTCGATGGACTACGGCGAGTACATGAACCGCCGTCTC
CACGGATGGGGATTCGCCAAACTCCACGCGCAGATACGATACAAAGCCGTCGAGAAGGGTATCCCCGTCAAGACGGTGAACCCTCGCAACACCTCGAAAG
AGTGCCATGCGTGCGGTGAGGTAGGATACCGCCCACAACAGGCGACGTTCAAGTGTACGAACGATGCGTGCTGGATGGGCGAGTACCAAGCGGACGTGAA
CGGAGCGGTGAACATCGCAGACCGCTACCTCAGCGGAGAGAGTCGTTTCAGAGAACACGAGAACGACGATGACTCGGCTGAGGATGGGGGGCGTTTGACC
GCCCCACAAGACAGCCAAGCCGATGCTACCAAGCAAGAGACGCTTGGAACGTATGCGTCTTGAAACCGTAGGGCCACGCTCGGCCTGAAATCCCGTGGTG
GGATTCCCGCGTCTTCAGGCGCGGGAGGAGGTCAA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
390 bp	129 aa	142	531	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKTTRHATYNLNYHLVWLPKYRNSVLVNEVADRVRTILHEIADDKGLEILDLTVQPDHVHLFVSSPPKHAPSLLANWFKGISSRKYNHRYADHDGEKIGW
VRGYYAGTAGHVSSETVKNYIQRHEEDES

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1236 bp	411 aa	528	1763	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTTLTKTLELKLVEPNAHKRRKLRETREAYQQALHDAFDAGCTTQTEANDVVVNYKLSGYAKNALKKYVPQLTTTYNAGELHDAHPVRFTNEGLRLDHKP
ENAIEWYVKIPHHEDYHLWMPAQPNPEQRDWLEALNAGDATMGESRLFERDGTWYLHVTATRDAEGGSEVSDEERTPIGVDIGEASLVTVCHRDDHGCPT
APELWADEGKTVRRLRKTYFTATRRLQTRGSERIAESFGDDLWNQIDDVFHRVTREVVDYAESVKNPVLVLEDLTYIRESMDYGEYMNRRLHGWGFAKLH
AQIRYKAVEKGIPVKTVNPRNTSKECHACGEVGYRPQQATFKCTNDACWMGEYQADVNGAVNIADRYLSGESRFREHENDDDSAEDGGRLTAPQDSQADA
TKQETLGTYAS

Blast result :

Comments

ISHla21 is 94% (TnpA : the transposase) aa similar to ISNpe10 and 95% (TnpB) aa similar to ISNamo17.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. (2009) Direct submission GenBank.