ISHma17

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY596297 ND Haloarcula marismortui
Haloarcula marismortui ATCC 43049
DNA section
IS Length : 2009 bp

Ends


Left end : AGAGATACGAGCGCATACCCCCGCCTTCCCGTGAGTGGCGAGTGTTCCGACGCTCTGTCGGACCGAGGAGCGAACAGGCGGGGGTCGAGCGGACAGCATA II struct. : No
Right end : GACGCGTGGAACGTATGCGTCTTGAAACCAACAGGTCGCTACACTCGGCCTGAAATCCCGTGGTGGGATTCCTCCGCGTTCACGCGGAGGAAGAGGTCAA II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GAGGTGGTCCGCTGATATGCGGTATCAT

DNA sequence

AGAGATACGAGCGCATACCCCCGCCTTCCCGTGAGTGGCGAGTGTTCCGACGCTCTGTCGGACCGAGGAGCGAACAGGCGGGGGTCGAGCGGACAGCATA
GTGTGACAGTCCACCGTTCTATTGCAGGGCTGGATTTCCTACCGTTTCCGCAAGACATTTGCCTGACAGGACCATAGTGTGTAGCACGGGTGAAGACCAC
ACGGCACGCAACCTACAACCTCAACTACCACATAGTGTGGCTCCCGAAGTCTCGCAGAACCGACGGTTCTGCGGATATCGCGGAACGCAGTTCCGCTCAA
TACCGTAAGGCGGTACTGACCGGAGAGGTTGCCGACCGTGTGGAATCCATCCTCCACGAAATTGCCGACGAGAAGGGCTTGGACATTCAAAACCTGACTG
TTCAGCCCGACCACGTTCATTTGTTCGTCAGTAGCCCGCCCAAACACAGCCCATCGCTGCTCGCCAACTGGTTCAAGCGCATTTCCTCACGGAAGTACAA
TCACCGCCACACCGACCACGACGGCGAGAAAATCCGGTGGGCACGCGGCTACTACGCCGGCACAGCCGGGCACGTCTCCAGCGAAACTGTCGAGAACTAC
ATCGACCGACACAAGGAGGTCCAAGCGTGACCGAACTCACGAAAACGCTCGAACTGAAGCTTGTGGACCCGAACACCCACAAGCGACAGAAGCTTCGTGA
GACACAGGACGCGTACCAGCAGGCACTCCAAGCCGCTTTCGCCGCTGGCTGTGACACACAGTCAGCGGCCAACGACGTGGTTGTTGAGTACGACCTGAGC
GGCTACGCGAAAAACGCCCTCAAGAAGTACGTCCCACAACTCTGTGGGAACAGCTATGACGCCGACGAGCTTCACGACAACCACCCGGTGCGGTTCACCA
ATGAGGGATTGCAACTTGACCACCAGCCACAGAACGCCATCGAGTGGTACGTCAAAATCCCGCACCACGAGGATTACCACCTCTGGATTCCAGCACGCGC
TAATCCCGAACAGCGGGAGTGGCTCGAAGCGTTAGACGCAGACGACGCCGAGATGGGTGAAAGTCGGCTGTTTGAGCGGGACGGAACGTGGTATCTCCAC
ATCACCGTTACCCGCGACGTGGAGGACCAATCTGAGGCGTCCGCCGACGAGCGGACACCGCCAGACGAGTCTGGCGAGCCCTGTCTCGCTGGACTCGACA
GGACGCCGATAGGCGTGGATATTGGAGAAGCGAGTCTCGTCACGGTGTGTCACCGCGACGGCTCTGGTTCTCCGGTTCGCCCCCGCCTGTGGGCCGACGA
CGGTAAAGCCGTTCGTCGGCTCCGCAAAACCTACTTCACCGCCAAGCGACGGCTTCAGCAGCGCGGCAGTGAGCGAATCGCGGAGTCCTACGGCGACTCG
CTGTGGGACCAGATTGACGACGTGTTCCACCGTGTAACCCGTGAGGTCGTGGAGTACGCCGAGTCTGTCGAGAACCCAGTGTTGGTGCTGGAAGACCTGA
CGTACATCCGCGAGAACATGGACTACGGCGAGTACATGAACCGCCGGTTGCACGGATGGGGGTTTGCCAAGCTCCACGCACAGATTCGCTACAAAGCCAC
AGAGAAGGGGATTCCCGTCGAGACGGTGAATCCCCGGAACACGTCGAAGGCGTTCCATGCCTGCGGTGAACACGGCTCCCGGCCACGACAGGCGACGTTC
AGATGCTCGAACGACGACTGCTGGCTCGGTGAGTATCAAGCCGACGTGAACGGGGCGATAAATATTGCAGACCGCTACCGTAGTGGAGAGAGTCACCGCC
GAAGCGACCGGAGTTCCCGGCAGAAGGCCGGTGACGATGACTCGGCTACGGATGGGGCCTCTTTGACCGGGCCACAAGACAGCCACGCCGATGCTGGAAC
CCAGCAGGAGACGCGTGGAACGTATGCGTCTTGAAACCAACAGGTCGCTACACTCGGCCTGAAATCCCGTGGTGGGATTCCTCCGCGTTCACGCGGAGGA
AGAGGTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
441 bp146 aa190630+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKTTRHATYNLNYHIVWLPKSRRTDGSADIAERSSAQYRKAVLTGEVADRVESILHEIADEKGLDIQNLTVQPDHVHLFVSSPPKHSPSLLANWFKRISS
RKYNHRHTDHDGEKIRWARGYYAGTAGHVSSETVENYIDRHKEVQA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1308 bp435 aa6271934+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTELTKTLELKLVDPNTHKRQKLRETQDAYQQALQAAFAAGCDTQSAANDVVVEYDLSGYAKNALKKYVPQLCGNSYDADELHDNHPVRFTNEGLQLDHQ
PQNAIEWYVKIPHHEDYHLWIPARANPEQREWLEALDADDAEMGESRLFERDGTWYLHITVTRDVEDQSEASADERTPPDESGEPCLAGLDRTPIGVDIG
EASLVTVCHRDGSGSPVRPRLWADDGKAVRRLRKTYFTAKRRLQQRGSERIAESYGDSLWDQIDDVFHRVTREVVEYAESVENPVLVLEDLTYIRENMDY
GEYMNRRLHGWGFAKLHAQIRYKATEKGIPVETVNPRNTSKAFHACGEHGSRPRQATFRCSNDDCWLGEYQADVNGAINIADRYRSGESHRRSDRSSRQK
AGDDDSATDGASLTGPQDSHADAGTQQETRGTYAS

 

Blast result :
Comments
ISHma17 is 79% (TnpA : the transposase) aa similar to ISNpe10 and 91% (TnpB) aa similar to ISNma24.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Baliga,N.S., Bonneau,R., Facciotti,M.T., Pan,M., Glusman,G., Deutsch,E.W., Shannon,P., Chiu,Y., Weng,R.S., Gan,R.R., Hung,P., Date,S.V., Marcotte,E., Hood,L. and Ng,W.V. (2004) Genome Res. 14 (11), 2221-2234