ISfinder Mirror Site

ISHall1

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP007059	ND	Halostagnicola larsenii	Halostagnicola larsenii XH-48

DNA section

IS Length : 1866 bp

Ends

Left end : AGGAAACAGCGAGAGTTCCACGGGTCAGGTGACCCGTGGGTGAATCGCGTACGCTTGCCTAATGTTCAGTCTCCTCGATGTACCGACGAACCACGTCCTC II struct. : No
Right end : TGCCCATCCTCATCCCGCGAAATCGCTTCGTGTGGAGTGCTTACGACGTAGGCTGAGGGAAGTAGAAAAGCCACGGGTCACCCGACCCGTGGTCGTTTAC II struct. : No

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GTTTCATCCAGT	TCTAA	TCTGAAATAAGT

DNA sequence

AGGAAACAGCGAGAGTTCCACGGGTCAGGTGACCCGTGGGTGAATCGCGTACGCTTGCCTAATGTTCAGTCTCCTCGATGTACCGACGAACCACGTCCTC
GGAAACAGCACCAGTCGTCCCAACGTAGTATCCATCCTTCCACAACCCACTTCTCCAGAAATACCGCTGTTTGATTTCTGGATGGTGTTTCAGAATCGTT
CGGCCCGAGTAGCCCTTGAACTGCTTGGCAATCTCGGCAGGACTCCATTTCGGGTCAGCCTCCACGAACAAGTGAACGTGGTCAGTAGCAATCTCCATCG
ACACAATCTCGTGACCGAACCGCTCAGCAGTTCCTTGGAACAACTCCGCAAGTTCGTCTCGAACTAACTCCAACATCCCGTGTCGATACTTCGGACACCA
CACGAAATGATACTTACACAAACTAATCGAATGTGTATGACTCCTGTATTCTCCCATCCTAATCCCTACTTTTATTATCATTTGATTCGATAGTCAAGGT
ATGGGTATCGAAGAAGTCACGAAGACCGCACGCACCCGGCTCTGCAGAGAGTCTGGTGAGCGGTCGTGGCTCAAAGATGCCCGTTACACCGCACGAGACA
TCGCCAACGACACACTCCGACTCAAACGACAAGGCTACAACCGCACCGAGATTCAGAACGAAGTTGACCGCGATGACTTCCTTCGGAACAATAAGTGTGC
AGTCGTCGGGAAAGCCCTGCAAGCGTGGGACTCCTACAAAGAACTCCTCAATTGGTGGTACGACCAAGACGACACCAACGTCGGGAAACCGTCGCCACCA
ACCACGGACAAGAAAGGAGCCTACCCGCTCGTTATGGCGCACACCGAAGGTTATCGACTCACCTACAACGATGAGGCCGACCGCATTCAGTTCCGTGTGA
GTCCGAAGCCGTACAAGAAGGTGAAAGGACACCTTCGAGGACGACCGGAAGACCTCAACCTCATCGAGTCAGCCTTGACCGAAGATGAATGGTCGTTGGG
ACAGGCCGAACTTCTGTACCGAGATGGCGTGTACTACCTACACGTCACGGTCAAAACAGAAGTCGAAGTGCCTGAACCCGAAGACGCTGATACATTCGTC
GGTGTGGACATTAACGAGCGCAACATCGCTCTCACCGCCCTTAATCGTGAGACGATGGACACGCTCGGAACACTCGTGCTTGACTACGGCTTGGTGAAAG
CCGAACGCCAACGCTACCACACCATCACCAAACGCTGTCAAGAACACGGCCAACACTCCATCCACCACCAACTCGGGGAGAAAGAAGAACGCTACACTGA
GTGGACTCTTCACAGACTGTCCCGAGTTGTGGTGGAGTTCGCCCAAAAATTCCCTAACCCAGTCATCGTGTTCGAGGACTTGAGTGGGATTCGAGACGCC
ATCAAGTACGGCACGTACATGAACCGTCGTCTACATAAACTGCCGTTCCACAAGTTCGAGCAACAAGTTCGATACAAAGCAACGTGGAATCAGATATCGT
GTGAGACGGTTGAGTCGCCGTACAACTCGAAGTCGTGTTCGTGCTGTGGACACCGTGGCTACCGCCAAGGCCGGCGGTTCCGGTGTACGAACGAGTCGTG
CGAGGTTCAGCAAGACCACGCTGACCGAAACGCGAGCGTGAATGTGGCGTGGCGAGCGTGGGCAAAACACGCTGGCGTAGACGTTGAATCGGTTAATTAC
CGGACTCGCAAAACCCAACCGTTTGTTCGGAAGGTGAGCCTGTCTGGGTCGGGGCACTCTGTAAACTGCCCATCCTCATCCCGCGAAATCGCTTCGTGTG
GAGTGCTTACGACGTAGGCTGAGGGAAGTAGAAAAGCCACGGGTCACCCGACCCGTGGTCGTTTAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
399 bp	132 aa	457	59	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGEYRSHTHSISLCKYHFVWCPKYRHGMLELVRDELAELFQGTAERFGHEIVSMEIATDHVHLFVEADPKWSPAEIAKQFKGYSGRTILKHHPEIKQRYF
WRSGLWKDGYYVGTTGAVSEDVVRRYIEETEH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1317 bp	438 aa	501	1817	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MGIEEVTKTARTRLCRESGERSWLKDARYTARDIANDTLRLKRQGYNRTEIQNEVDRDDFLRNNKCAVVGKALQAWDSYKELLNWWYDQDDTNVGKPSPP
TTDKKGAYPLVMAHTEGYRLTYNDEADRIQFRVSPKPYKKVKGHLRGRPEDLNLIESALTEDEWSLGQAELLYRDGVYYLHVTVKTEVEVPEPEDADTFV
GVDINERNIALTALNRETMDTLGTLVLDYGLVKAERQRYHTITKRCQEHGQHSIHHQLGEKEERYTEWTLHRLSRVVVEFAQKFPNPVIVFEDLSGIRDA
IKYGTYMNRRLHKLPFHKFEQQVRYKATWNQISCETVESPYNSKSCSCCGHRGYRQGRRFRCTNESCEVQQDHADRNASVNVAWRAWAKHAGVDVESVNY
RTRKTQPFVRKVSLSGSGHSVNCPSSSREIASCGVLTT

Blast result :

Comments

ISHall1 is 83% (TnpA) and 94% (TnpB) aa similar to ISHgi1.

References

1] Friedhelm Pfeiffer (2015) Direct submission
2] Anderson,I., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2014) Direct submission GenBank.