ISNti1
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ARPH01000002 | ND | Natronorubrum tibetense | Natronorubrum tibetense GA33 |
DNA section
IS Length : 1474 bp
Ends
Left end : ATAAGAAGTAGCGCGAGTACCCCGACCCACCGTGGTCGGGGGTGAAGCGCGTCTAACCCGTACTTTATCAATCACTGAACCGTACCCGAATACAGTGCAG II struct. : Yes
Right end : AGACTCGCAGAGTCGAGTGCGAGAGGAATTAAAGTCCGTTGCGCCAGCAGTACAGGACTAAACACGGACGCCCCGCGCCACCGTGCGCGCGGTACTTCAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| TGAATGTTACTC | TCTAT | CGCAAGACGGCA | tcac |
DNA sequence
ATAAGAAGTAGCGCGAGTACCCCGACCCACCGTGGTCGGGGGTGAAGCGCGTCTAACCCGTACTTTATCAATCACTGAACCGTACCCGAATACAGTGCAG
TCGTCCAAACTCACCCAAACCCTGTCGTTCGGATTAGACATTGACACGGGGAGTGCCGACGACTTGCAAGACAGCTGTCTCGAAGCCCGACGCATCCGTA
ACGAAACCAACCGCCTCGATAAACAAGGCTGGGACTGGAAAGAACTCAAATCTGTCGTCGTGGACAATGCGGATCACGTTAAAAACACCAGTCAACTCAT
CGTAGACAAAGCCCTCGGTGAAATCGAAACCTACCACGACAACAAAGACAACAATTGGGGCCGACCATACCCATACATCGACGGACTGTACCCGATGGTC
ATGAACCATAAAGAAGGGTACCGACTTTTCCTCGAAGATGATGATACGATTCGGTTCCGTATCAGTGCTGCCCCACGCAACCACGTCAAAGGCGAACTTC
GTGGTAATCCAGAGCATTTCGACCGGGTGCGAACCGCCCTCACATACGATGACTGGCGCGTTGGAACCGCTGAAGTCATCTACAAACATGACGAATGGCG
ACTGCATGTCACCGTCACACACGAATACCACAAAGTCGCCAGTAAAACAGATGCAGACACAATCGTTGGTGTGGATGTGAACGAGGATTGCGTTGCTCTT
GCTGCGATGAATCGAAACGGCTCAGTAATGGACTCAGTGGTGATTGAATACCCTGAAATCAAAGAACAACGCCACGAGTTCTTCACAAAACGGAAGCGGA
TGCAGAAAGTCGGACAGACAGCATTTGATTTGGTCGTGCAGACCGAGGAACGCGACTATGTTCACGACTGTCTGCACAAGGTGTCGCGTCAGGTAGTTAA
TTGGGTGTCGCAGTTCACGGACCCGATTATTGTCTTTGAAGACCTCAAAGACATGCGAGACTCAATCGACTACGGAACGCGAATGAACCGCCGCCTACAC
AGCCTGCCCTTCGCCGCACTCCGAGATATGGTGTCGTATAAAGCCGCATGGAACGGTATTCCGTCGGATGATGTTGACCCGGAATATACGAGTCAGCAGT
GTCCACGGACTGAGTGTCAGCATACCTCTCGGTCGAACCGTTATAAGAAGCGGTTTAAATGCGGTGAGTGTGGATTTCAGGATCATGCTGATCGGAAGGC
GGCGGTTTGTGTAGTGCAGGAGTGGTTTGGAAAGCAAGATGGAAATGTGCCGTCTCTCGAAACCCTTCCACGGGTGTGGAAGGTGAGATGGGCGGCATCG
GGTCGTGGTGGAGCGACCGACTCTCACGGACCTGTTTTGGGTTCGGGTGTTCACCGACACGGAACGTCGGCGCAAGACTCGCAGAGTCGAGTGCGAGAGG
AATTAAAGTCCGTTGCGCCAGCAGTACAGGACTAAACACGGACGCCCCGCGCCACCGTGCGCGCGGTACTTCAC
TCGTCCAAACTCACCCAAACCCTGTCGTTCGGATTAGACATTGACACGGGGAGTGCCGACGACTTGCAAGACAGCTGTCTCGAAGCCCGACGCATCCGTA
ACGAAACCAACCGCCTCGATAAACAAGGCTGGGACTGGAAAGAACTCAAATCTGTCGTCGTGGACAATGCGGATCACGTTAAAAACACCAGTCAACTCAT
CGTAGACAAAGCCCTCGGTGAAATCGAAACCTACCACGACAACAAAGACAACAATTGGGGCCGACCATACCCATACATCGACGGACTGTACCCGATGGTC
ATGAACCATAAAGAAGGGTACCGACTTTTCCTCGAAGATGATGATACGATTCGGTTCCGTATCAGTGCTGCCCCACGCAACCACGTCAAAGGCGAACTTC
GTGGTAATCCAGAGCATTTCGACCGGGTGCGAACCGCCCTCACATACGATGACTGGCGCGTTGGAACCGCTGAAGTCATCTACAAACATGACGAATGGCG
ACTGCATGTCACCGTCACACACGAATACCACAAAGTCGCCAGTAAAACAGATGCAGACACAATCGTTGGTGTGGATGTGAACGAGGATTGCGTTGCTCTT
GCTGCGATGAATCGAAACGGCTCAGTAATGGACTCAGTGGTGATTGAATACCCTGAAATCAAAGAACAACGCCACGAGTTCTTCACAAAACGGAAGCGGA
TGCAGAAAGTCGGACAGACAGCATTTGATTTGGTCGTGCAGACCGAGGAACGCGACTATGTTCACGACTGTCTGCACAAGGTGTCGCGTCAGGTAGTTAA
TTGGGTGTCGCAGTTCACGGACCCGATTATTGTCTTTGAAGACCTCAAAGACATGCGAGACTCAATCGACTACGGAACGCGAATGAACCGCCGCCTACAC
AGCCTGCCCTTCGCCGCACTCCGAGATATGGTGTCGTATAAAGCCGCATGGAACGGTATTCCGTCGGATGATGTTGACCCGGAATATACGAGTCAGCAGT
GTCCACGGACTGAGTGTCAGCATACCTCTCGGTCGAACCGTTATAAGAAGCGGTTTAAATGCGGTGAGTGTGGATTTCAGGATCATGCTGATCGGAAGGC
GGCGGTTTGTGTAGTGCAGGAGTGGTTTGGAAAGCAAGATGGAAATGTGCCGTCTCTCGAAACCCTTCCACGGGTGTGGAAGGTGAGATGGGCGGCATCG
GGTCGTGGTGGAGCGACCGACTCTCACGGACCTGTTTTGGGTTCGGGTGTTCACCGACACGGAACGTCGGCGCAAGACTCGCAGAGTCGAGTGCGAGAGG
AATTAAAGTCCGTTGCGCCAGCAGTACAGGACTAAACACGGACGCCCCGCGCCACCGTGCGCGCGGTACTTCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1341 bp | 446 aa | 95 | 1435 | + | No |
AG : TnpB
ORF sequence :
MQSSKLTQTLSFGLDIDTGSADDLQDSCLEARRIRNETNRLDKQGWDWKELKSVVVDNADHVKNTSQLIVDKALGEIETYHDNKDNNWGRPYPYIDGLYP
MVMNHKEGYRLFLEDDDTIRFRISAAPRNHVKGELRGNPEHFDRVRTALTYDDWRVGTAEVIYKHDEWRLHVTVTHEYHKVASKTDADTIVGVDVNEDCV
ALAAMNRNGSVMDSVVIEYPEIKEQRHEFFTKRKRMQKVGQTAFDLVVQTEERDYVHDCLHKVSRQVVNWVSQFTDPIIVFEDLKDMRDSIDYGTRMNRR
LHSLPFAALRDMVSYKAAWNGIPSDDVDPEYTSQQCPRTECQHTSRSNRYKKRFKCGECGFQDHADRKAAVCVVQEWFGKQDGNVPSLETLPRVWKVRWA
ASGRGGATDSHGPVLGSGVHRHGTSAQDSQSRVREELKSVAPAVQD
MVMNHKEGYRLFLEDDDTIRFRISAAPRNHVKGELRGNPEHFDRVRTALTYDDWRVGTAEVIYKHDEWRLHVTVTHEYHKVASKTDADTIVGVDVNEDCV
ALAAMNRNGSVMDSVVIEYPEIKEQRHEFFTKRKRMQKVGQTAFDLVVQTEERDYVHDCLHKVSRQVVNWVSQFTDPIIVFEDLKDMRDSIDYGTRMNRR
LHSLPFAALRDMVSYKAAWNGIPSDDVDPEYTSQQCPRTECQHTSRSNRYKKRFKCGECGFQDHADRKAAVCVVQEWFGKQDGNVPSLETLPRVWKVRWA
ASGRGGATDSHGPVLGSGVHRHGTSAQDSQSRVREELKSVAPAVQD
Blast result :
Comments
ISNti1 is 85% (TnpB) aa similar to ISNma15.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Anderson,I., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2013) Direct submission GenBank.
2] Anderson,I., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2013) Direct submission GenBank.