ISfinder Mirror Site

ISHasp2

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP007061	ND	Halobacterium sp.	Halobacterium sp. DL1

DNA section

IS Length : 1671 bp

Ends

Left end : CAGTTTGTAGGCGGTAAGCCCCCTTCCTCAAGGAGCGACCAGAGGGAGCGAGTAGGGAGGGATACACCGTCCCCAGAAATCTTCGATTTCTGGTGTGCGA II struct. : Yes
Right end : TCAAAGACTTCGGGTTAGCAGTCAGAACTCGGAGAGTTCTGACGACACCCTCAAGGAGCGAACGGCGTCAGCCGTGAGCGAGTAGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GAAACCAGCGAA	TTAG	CGGAGGCGGCCG	tcac

DNA sequence

CAGTTTGTAGGCGGTAAGCCCCCTTCCTCAAGGAGCGACCAGAGGGAGCGAGTAGGGAGGGATACACCGTCCCCAGAAATCTTCGATTTCTGGTGTGCGA
ACGAATCGCGCAGCGATTCGTCCACGCCGTCATCATATTACAAACACGTTCGACCCCACCACCCAACCATCCCATCGATACAGTTAAGTTGATATAATGT
ATAAACACAGGTGATGGATAGGCACGAAATAGAAGGCCACGAAGTCATCGAGGGAGAAGTGAAGCCGACCGGGAACGGCGCACACGTTCTCGTCCCGAAA
CGGTGGCGAGGAGCTGACGTGAAAATCGTCCGAACGTCTGATCCCGACGAATAGCCAATGCACTACACCTACAGATTTCGGCTTGATCCCACGCCTGAAC
AGCGTGAACTGTTGGATCATCACCGAGATACCTGTAGGCAACTCTACAACCACGCACTCAACGAATTCAAGCAAATTCCCAAATCGGCGGGTACACTTAA
CCAACGAGTGCGACAAGTACGCGATCAGCTCACCAGCCTCAAAGACTGGTGGGATGAGCTGAACGATGTCTATTCAACGGTCGCACAAGCTGCTGTCATG
CGTATCGAAGACAGCATCAAAGCCCTCTCTCAGTTGAAGCAGAACGGCTACAACGTGGGCAGTCTCAATTGGAAGGCCCCCAAGGATTTCCGTAGTTTCA
CCTACATACAGTCTGGCTTCGAGTTCGATAGTAAGAACGGCCAACCCGTACTGTCGCTGTCGAAACTTGCGGATATTCCCCTCATCAAACACCGCGCAAT
TCCTGACGCCGAGACTGTCAAAGAAATCACGATTAAGAAGGAGTCAACCGGTGAATGGTTCGCTTCATTCACCGTCGGCGATAAAGAGACTCCTGAGAAA
CCGACCGACCCAGATCGATGTGTCGGGATTGACGTTGGCATCTTGAAGTACGCCCATGACACAGACGGCACCGCCGTCGAATCGCTCGACTTATCTGACG
AACGCGAGCGGTTGGAACGCGCACAGCATGATCTTTCGCAGAAGGAACGCGGTTCCGCGAATTGGGAGAGACAACGGCAAGTTGTGGCCGAGCGCCACGC
CGATCTCAAGCGAAAGCGTCGTGACTTCCTTCACAAACTCTCGAACTACTACGCCACCGAATACGACCTCGTAGCGGTCGAAGGCCTCGACGCGAAGGAG
TTGGTCGAACTCCCCGGAAACTCACGGAATCGGGCGGGAGCGGCGTGGGGAACGTTCCTTCGAATGCTTGAGTACAAGTGCGAACGCGAAGGAACACACT
TTGCCGAAGTCGATCCAAGGAACACGACGAAAGCGTGCGCGTCTTGCGGCGTCAAGACGGACAAGCCGTTGTGGGTTCGTGAACACTCGTGTCCCTCGTG
TGGGTTTGAGGCGGACAGGGACGCGAACGCAGCGTGGAACATTCTTTCTCGCGGTCTTAAAAATATAGGAGTGGTTCACTCCGAATCAACGCCTGTGGAG
ACTGCGCTCCCTACGGACACCGTTGTGTCTGCAAAGCGCGTCATCGAAACAGGAAGCCCCATCACCAGAAGTCAAAGACTTCGGGTTAGCAGTCAGAACT
CGGAGAGTTCTGACGACACCCTCAAGGAGCGAACGGCGTCAGCCGTGAGCGAGTAGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
141 bp	46 aa	214	354	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDRHEIEGHEVIEGEVKPTGNGAHVLVPKRWRGADVKIVRTSDPDE

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1299 bp	432 aa	358	1656	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MHYTYRFRLDPTPEQRELLDHHRDTCRQLYNHALNEFKQIPKSAGTLNQRVRQVRDQLTSLKDWWDELNDVYSTVAQAAVMRIEDSIKALSQLKQNGYNV
GSLNWKAPKDFRSFTYIQSGFEFDSKNGQPVLSLSKLADIPLIKHRAIPDAETVKEITIKKESTGEWFASFTVGDKETPEKPTDPDRCVGIDVGILKYAH
DTDGTAVESLDLSDERERLERAQHDLSQKERGSANWERQRQVVAERHADLKRKRRDFLHKLSNYYATEYDLVAVEGLDAKELVELPGNSRNRAGAAWGTF
LRMLEYKCEREGTHFAEVDPRNTTKACASCGVKTDKPLWVREHSCPSCGFEADRDANAAWNILSRGLKNIGVVHSESTPVETALPTDTVVSAKRVIETGS
PITRSQRLRVSSQNSESSDDTLKERTASAVSE

Blast result :

Comments

ISHasp2 is 87% (TnpB) aa similar to ISHli13. The first ORF is a passenger gene annotated as hypothetical protein.

References

1] Pfeiffer, F. (2015) Direct submission
2] Cavicchioli,R., Huntemann,M., Han,J., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2014) Direct submission GenBank.