ISHwa33
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AM180088 | ND | Haloquadratum walsbyi | Haloquadratum walsbyi DSM 16790 |
DNA section
IS Length : 1396 bp
Ends
Left end : AGAAAGCACGAGGCGAAAACCCACGGCTTTACTCGGCTTTAGTCGTGGGATGAAGCCGACAACTGGGAATCAAACCACGCTTCAGTAACAGGCCGACTCC II struct. : Yes
Right end : CGGGACGTTGAACGTGAACGGAGGCTATTCTCCTGCCTCGTCAGAGGCCAGAACGGGAGTCCACGCTGAATCCCACCGCTTTTAGCGGTGGGTAGCTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| GGCAAAATCGTT | TTAT | TTAGATGTCATC | tcaa |
DNA sequence
AGAAAGCACGAGGCGAAAACCCACGGCTTTACTCGGCTTTAGTCGTGGGATGAAGCCGACAACTGGGAATCAAACCACGCTTCAGTAACAGGCCGACTCC
CCAATATTTAATAATCACCCATTTTACACATAAAATATGGGAATGCGGCGTACTGTCCCCGTTGCGCTCGATGTAGACAGTGACGACGCCGCACTCCTCG
AAGACACCGTAGACACGTTCCTCTGGTGCGCTCAGTACGTCGTAGACCACGCTTTCCAAGGCGAGTACGTCACCACCAGCAAGACCATGCTTGATGACGA
AACCTACGACGACGTGCGCGAGGAAACAGACGGGTTCAACGGTGGACTCGTCCAAGCCGCTCGGAACAAGGCCGCCGAAGCCTGCAAAAGCGTCGTCGCA
CGGTGGAAAAACGGCAAGAAAGCGTCGAAGCCGACGTTCACCAGCCCACACGTTGTCTACGACCACCGTACCGCGACGTTCCACGACGACTACGTGAGTC
TCGCCACCACAGACGGTCGTATCGAAGCCGACTCCATTCTGCCCGACGAGGACAGTGAGACACCGCACTCGGAGTACCTGTTTTCAGATGAGTATGAAAC
AACGGGTGCGGAACTGCATTATCACGACGGTGACTGGGTGCTTCACGTCCACTGCAAGACGGAGGTGGAGTCCGACACGTCGGAACAGCCAACGCCTGAG
AACGGAACGGTTCTCGGGGTTGACCTCGGCGTGAACAACCTCGCCGTAACTTCGACGGGCACGTTCTGGACGGGCGAGGAGTTCGACCACTGGCGCAGAG
AATACGAGAAACGGCGTGGGTCGCTCCAACAGTGCGGGACTCGATGGGCACACCAGAACATCCAATCAGTCGGACGGAAAGAGGAAGGTCGATTCAAGCT
GATGCTGCACCGCATCAGCAACGAATTGCTCGCGGAGGCTCGTGAAAACGAGTGTTCGGTGATAGCGTTCGAGGATTTGACCGACATTCGTGAGCGGACT
GGCGCGTCGTGGGGTCACAAGTGGGCGTTTGACCGACTGTACGAGTACGTTGAGTACAAGATCGAAGAATACGGTATCTTCGTGGAGCAGGTTGACCCTG
AGAACACGTCACGGCGTTGCTCGCACTGCGGGTTCACTCATCCAGACAACCGCGAGGACGAAGACTTCGAGTGCCTGAAGTGCGGGTATGAAAATCACGC
GGATTACAACGCTGCGAAGAACATCGGGTTGCGGTATCTCCGTCGCAACCAAACCGGGGGCGACGGAGGCGCACTCTTGGGCGTGCGCTTGAACAGCGGG
ACGTTGAACGTGAACGGAGGCTATTCTCCTGCCTCGTCAGAGGCCAGAACGGGAGTCCACGCTGAATCCCACCGCTTTTAGCGGTGGGTAGCTCAA
CCAATATTTAATAATCACCCATTTTACACATAAAATATGGGAATGCGGCGTACTGTCCCCGTTGCGCTCGATGTAGACAGTGACGACGCCGCACTCCTCG
AAGACACCGTAGACACGTTCCTCTGGTGCGCTCAGTACGTCGTAGACCACGCTTTCCAAGGCGAGTACGTCACCACCAGCAAGACCATGCTTGATGACGA
AACCTACGACGACGTGCGCGAGGAAACAGACGGGTTCAACGGTGGACTCGTCCAAGCCGCTCGGAACAAGGCCGCCGAAGCCTGCAAAAGCGTCGTCGCA
CGGTGGAAAAACGGCAAGAAAGCGTCGAAGCCGACGTTCACCAGCCCACACGTTGTCTACGACCACCGTACCGCGACGTTCCACGACGACTACGTGAGTC
TCGCCACCACAGACGGTCGTATCGAAGCCGACTCCATTCTGCCCGACGAGGACAGTGAGACACCGCACTCGGAGTACCTGTTTTCAGATGAGTATGAAAC
AACGGGTGCGGAACTGCATTATCACGACGGTGACTGGGTGCTTCACGTCCACTGCAAGACGGAGGTGGAGTCCGACACGTCGGAACAGCCAACGCCTGAG
AACGGAACGGTTCTCGGGGTTGACCTCGGCGTGAACAACCTCGCCGTAACTTCGACGGGCACGTTCTGGACGGGCGAGGAGTTCGACCACTGGCGCAGAG
AATACGAGAAACGGCGTGGGTCGCTCCAACAGTGCGGGACTCGATGGGCACACCAGAACATCCAATCAGTCGGACGGAAAGAGGAAGGTCGATTCAAGCT
GATGCTGCACCGCATCAGCAACGAATTGCTCGCGGAGGCTCGTGAAAACGAGTGTTCGGTGATAGCGTTCGAGGATTTGACCGACATTCGTGAGCGGACT
GGCGCGTCGTGGGGTCACAAGTGGGCGTTTGACCGACTGTACGAGTACGTTGAGTACAAGATCGAAGAATACGGTATCTTCGTGGAGCAGGTTGACCCTG
AGAACACGTCACGGCGTTGCTCGCACTGCGGGTTCACTCATCCAGACAACCGCGAGGACGAAGACTTCGAGTGCCTGAAGTGCGGGTATGAAAATCACGC
GGATTACAACGCTGCGAAGAACATCGGGTTGCGGTATCTCCGTCGCAACCAAACCGGGGGCGACGGAGGCGCACTCTTGGGCGTGCGCTTGAACAGCGGG
ACGTTGAACGTGAACGGAGGCTATTCTCCTGCCTCGTCAGAGGCCAGAACGGGAGTCCACGCTGAATCCCACCGCTTTTAGCGGTGGGTAGCTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1245 bp | 414 aa | 137 | 1381 | + | No |
AG : TnpB
ORF sequence :
MGMRRTVPVALDVDSDDAALLEDTVDTFLWCAQYVVDHAFQGEYVTTSKTMLDDETYDDVREETDGFNGGLVQAARNKAAEACKSVVARWKNGKKASKPT
FTSPHVVYDHRTATFHDDYVSLATTDGRIEADSILPDEDSETPHSEYLFSDEYETTGAELHYHDGDWVLHVHCKTEVESDTSEQPTPENGTVLGVDLGVN
NLAVTSTGTFWTGEEFDHWRREYEKRRGSLQQCGTRWAHQNIQSVGRKEEGRFKLMLHRISNELLAEARENECSVIAFEDLTDIRERTGASWGHKWAFDR
LYEYVEYKIEEYGIFVEQVDPENTSRRCSHCGFTHPDNREDEDFECLKCGYENHADYNAAKNIGLRYLRRNQTGGDGGALLGVRLNSGTLNVNGGYSPAS
SEARTGVHAESHRF
FTSPHVVYDHRTATFHDDYVSLATTDGRIEADSILPDEDSETPHSEYLFSDEYETTGAELHYHDGDWVLHVHCKTEVESDTSEQPTPENGTVLGVDLGVN
NLAVTSTGTFWTGEEFDHWRREYEKRRGSLQQCGTRWAHQNIQSVGRKEEGRFKLMLHRISNELLAEARENECSVIAFEDLTDIRERTGASWGHKWAFDR
LYEYVEYKIEEYGIFVEQVDPENTSRRCSHCGFTHPDNREDEDFECLKCGYENHADYNAAKNIGLRYLRRNQTGGDGGALLGVRLNSGTLNVNGGYSPAS
SEARTGVHAESHRF
Blast result :
Comments
ISHwa33 is 95% (TnpB) aa similar to ISNph22. In the genome, this transposon is targetted by
a mobile genetic element (MITE of ISHwa2, IS4 family IS50 group) after base 38. The MITE was in silico deleted in the sequence of this file.
a mobile genetic element (MITE of ISHwa2, IS4 family IS50 group) after base 38. The MITE was in silico deleted in the sequence of this file.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Bolhuis, H., Palm, P., Wende, A., Falb, M., Rampp, M., Rodriguez-Valera, F., Pfeiffer, F., and Oesterhelt, D. (2006) BMC Genomics 7, 169
2] Bolhuis, H., Palm, P., Wende, A., Falb, M., Rampp, M., Rodriguez-Valera, F., Pfeiffer, F., and Oesterhelt, D. (2006) BMC Genomics 7, 169