ISNin1

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AOHZ01000031 ND Natronolimnobius innermongolicus
Natronolimnobius innermongolicus JCM 12255
DNA section
IS Length : 1902 bp

Ends


Left end : ACGAAAAACAGCGCGAGTTCCCCGCCCTTCCGAAAACCTGCGGTTTTCGGCTTTCGCAAATCTTCGATTTGCGTCAACACCGTGGGCGGGGGTGAAGCGC II struct. : No
Right end : GCCGCCCAACCTCATCCCGCTCGCTTGCCGAGCAGGGAGTGCTAGCGCACGGCTGAGGGAATTAGAAAAGCCTCGGGCCACCGCGCCCGAGGTTGTTTAC II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CTCGAATCGACGTCTAATTTTCGGCGGGT

DNA sequence

ACGAAAAACAGCGCGAGTTCCCCGCCCTTCCGAAAACCTGCGGTTTTCGGCTTTCGCAAATCTTCGATTTGCGTCAACACCGTGGGCGGGGGTGAAGCGC
GTCACTCCAGCGCGTGTTCCGTCTCTTCTGTATACCGCTCAACCACTTCCTCCGATACCGCACCTGTCGTTCCCACGTAGTACCCAACCTTCCAGAATCC
ACCGCCCCAGAAATACGACTCCCGAATCTCGGGATACCGCTCCAGCAAGTGCTTACCCGAGTACGACTTGAACTGCCGGGCGAGGTCGGCTGGACTGTGT
TTTGGGTCGCACTGGACGAACAGGTGTACGTGGTCGTCTGCAATCTCCAACGCCAGAATTTTATGCCCGAAGTGGTCGGAGGTCTCACCAAACAACTCTC
GCACATCGTCTTCAACCACGTTGAGAATCGGGTGGCGGTACTTGGGACACCAGACAAAATAACACTTACAGGAACTAACCGAATGTGCATGACTGCGGTA
CTCTTCCATCCCTAATCCCTATATTTATGATGAGTTAGAACGAGTGTCAAAGCATGGGTGAAGAAGCCACGAAGACGATCCGGACGCGCCTCCACATAGC
GTCTGGTGAACGATCGTGGCTTCACAATGCCCGCCTCGCCTCACGCGAAATATTCAACCAAACCATCCGCCTCAAACAACAAGGGTACAATCGCACCGAG
ATACAGAAGGAGGTTGACCGCGACGACTTCCTACGGAACAACAAGTGCGCGGTCGTCGGCAAAGCCCTCCAAACATGGGACTCCTACCAATCACTCAAAG
ACTGGTGGGAGAACCAAGACGAGCCTGACGGAGGGAAGCCGACTCCGCCGAGTACGGACAAATCTGGTGCGTATCCGCTTGTGATGGCGCACACAGAAGG
GTACCGCCTCACCGTAGATGACGACACGAACCGCGTCCAGTTCCGCATCAGCCCGAAACCCTACAAGAAGGTGAAAGGACACCTGCGCGGCGAACCGGAC
GCAATGGACGAACTGCGAGACGCCCTCACGTCTGAAGAAGTGGATATTGGGCAGGCCGAACTCCTGTACCGCGATGGCGTGTACTACCTACACGTCACGG
TCACACGCGAGTTCGACGTCCCCGAACCTGACACCGCCGATACTGTGATTGGCGTGGACATCAACGAGCGCAACGTTGCTTTCACCGCCCTCGACCGCGA
CACGATGCAGACGAAAGGGACACTCGTCCTCGACTACGGACGAGTCAAGCAGGAACGCCAACGCTACCACACTATCACCACTCGCTGTCAAGAACACGGC
AAAACGAGCATCCACCACAGACTCGGTGACAAGGAGGAGCGGTTCACCGAGTGGGTGTTGCATCGGCTTTCCCGTGCTGTCGTGGAGTTTGCAGAGCAGT
TCTCGAACCCGGTTATCGTGTTCGAGAATATGAGCGGTATCCGCGACGAAATTAAGTATGGATCGTATATGAACCGTCGGTTGCACAAACTGCCGTTCCA
CAAGTTCGAGAAGTTCGTCTCGTACAAGGCGACGTGGCGAGAGATTCCTACGGATACGGTAGACGCGTACTACAACTCGAAGACGTGTTCGTGCTGTGGT
GAACGTGGCAGTCGGCAGGGGCGGCGGTTTCGGTGTACGAACGACGAGTGTGATGTGGTGCAAGACCACGCTGACCGGAATGCGTCGGTGAACATCGCGT
GGCGCGAGAAGGCGAAGCTTGACGGTAACGAATCGAATTACCGGACTCACAAAACCCAACCTCAGGTTCGGTTGGTGCGTTTGTCCGGGTCGGGGCGTGT
AAGCCGCCCAACCTCATCCCGCTCGCTTGCCGAGCAGGGAGTGCTAGCGCACGGCTGAGGGAATTAGAAAAGCCTCGGGCCACCGCGCCCGAGGTTGTTT
AC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
408 bp135 aa509102-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MEEYRSHAHSVSSCKCYFVWCPKYRHPILNVVEDDVRELFGETSDHFGHKILALEIADDHVHLFVQCDPKHSPADLARQFKSYSGKHLLERYPEIRESYF
WGGGFWKVGYYVGTTGAVSEEVVERYTEETEHALE

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1305 bp434 aa5541858+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MGEEATKTIRTRLHIASGERSWLHNARLASREIFNQTIRLKQQGYNRTEIQKEVDRDDFLRNNKCAVVGKALQTWDSYQSLKDWWENQDEPDGGKPTPPS
TDKSGAYPLVMAHTEGYRLTVDDDTNRVQFRISPKPYKKVKGHLRGEPDAMDELRDALTSEEVDIGQAELLYRDGVYYLHVTVTREFDVPEPDTADTVIG
VDINERNVAFTALDRDTMQTKGTLVLDYGRVKQERQRYHTITTRCQEHGKTSIHHRLGDKEERFTEWVLHRLSRAVVEFAEQFSNPVIVFENMSGIRDEI
KYGSYMNRRLHKLPFHKFEKFVSYKATWREIPTDTVDAYYNSKTCSCCGERGSRQGRRFRCTNDECDVVQDHADRNASVNIAWREKAKLDGNESNYRTHK
TQPQVRLVRLSGSGRVSRPTSSRSLAEQGVLAHG

 

Blast result :
Comments
ISNin1 is 96% aa similar to ISH34 (TnpA : the transposase) and 97% (TnpB) aa similar to ISNph6.
References
1] Friedhelm Pfeiffer (2015) Direct submission
2] Becker,E.A., Seitzer,P., Tritt,A., Larsen,D., Yao,A., Wu,D., Darling,A., Eisen,J.A. and Facciotti,M.T. (2012) Direct submission GenBank.