ISfinder Mirror Site

ISNpe30

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP003372	ND	Natrinema pellirubrum	Natrinema pellirubrum DSM 15624

DNA section

IS Length : 1528 bp

Ends

Left end : ACGCTGAACAGGCGCAATACCACGGCCTGAAGGCCGTGGATACGCGCCGTCACTCCGTGACTCATTCCACAGTCCCCGACAGGGCTGGATATTCTATTGT II struct. : Yes
Right end : TCCTGCGGATTCCAACCGCAGACAGAGGTGGTGGACTGCAAACCCTAATATCCCAACCGCGGTCGGGATTCCTCCGCCTTCAGGCGGAGGAGGATGTCAA II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
AATACGCTTTCT	TTAT	TCAGAGTAGTGG	tcaa
TGCCGAATGATA	TTAT	TACTATCTCGTT	tcaa

DNA sequence

ACGCTGAACAGGCGCAATACCACGGCCTGAAGGCCGTGGATACGCGCCGTCACTCCGTGACTCATTCCACAGTCCCCGACAGGGCTGGATATTCTATTGT
TCTTAATCCAAACTTTTACAATTTGAGCGAGTATAAGTGATTATAGAGGCGTTCCACGAATGCTGGAAGTCCACCGCACCCACCGAGCGAAAATCCGCAA
CCACTCACAGGTAGCGGACTCGCTCGACCGGCATGGCTGGAGTGTCAGCAAACTCTGGAACGTCGCCAACTACCACTCCCGAGAAGTCTGGGAGGAGACG
GGCAAAATTCCGGACCATAAGGAATTGAAAGACGAGTTGAAAGGTCACGACAAGTACAAGGGACTGCACAGTCAGTCCAGTCAGCGGGTTCTGGAGGAAC
TCGCTGAAGCCTTCCACTCGTGGTACTCCAGTGACGATGACAGGGACAATCCGCCCGGCTACCGCAAAGAAAACTACTACGACTCGCAAGGCCGTCGCGT
CCACGAAGAACACCCACGCAGCACGGTCACGTGGAAACAGAACGGCATCCGTCACGACACCAAGAATAACCGAGTTCGACTCTCGAAAGGGGCGAACCAC
AAGGAACACCCTCGAGCATGGGAATACATCCTTGTCGAGTACGAGACACGACCTGGAGTCACGGTCGAGAACCTGCAACAAGTCCGCGCCGTCTACGATC
AGCAGAACGAGCGGTGGGAACTCCACCTCGTCTGCAAAGACGAAATCGAGACACCCAACGCTCCCGGCGAGGAGACAGCAGGCATTGACCTTGGTATCAG
CAACTTCGCCGCTGTCGCATACAGCACAAACGAAGCCGACCTGTACCCCGGCAACCGCCTAAAGCAAGACGGGTACTACTTCCTGAAAGAAATCGCTAAC
TGTGACGACAGCGGTGGTGAACGGGCCGCCCGCTTGCACCACAAGTGGTCAGAACGCCGTACTCACTTCTTCCACAGTCTCGCCAAACACATCATCCAAC
GATGCGTGGAGAACGGCGTTTGCCGCATCAACGTCGGGGACTTGGAGGGGGTTCGAGAGGACGAACACGGCGAGTCGAAAAACTGGGGTAAGCACGGCAA
CCTCGACCTACACGGGTGGGCATTCGATCGCTTCACCAAGATTCTCACGTACAAGGCGAAGGTCGAGGGTATCGAAGTCGTAGAAGTGTCTGAACGGGAC
ACGAGTAAGACGTGTTGTATTTGCGGTAGGGAAGACGATAGTCAGCGTGTTCAACGCGGCTTGTACGTGTGCGAGGCGTGTGACGCGGCGTTCAACGCTG
ACGTGAACGGGGCTGAGAACATCCGTCTCAACATCAACGACGAAAGTAACTCCGAGTCTGCACCCAGTTTGGGTGGAGATAGGAGTACCGGCTGGTTGGC
ACAGCCCGAAGTCTACCTTCATAGCCTCTCCTGCGGATTCCAACCGCAGACAGAGGTGGTGGACTGCAAACCCTAATATCCCAACCGCGGTCGGGATTCC
TCCGCCTTCAGGCGGAGGAGGATGTCAA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1317 bp	438 aa	160	1476	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLEVHRTHRAKIRNHSQVADSLDRHGWSVSKLWNVANYHSREVWEETGKIPDHKELKDELKGHDKYKGLHSQSSQRVLEELAEAFHSWYSSDDDRDNPPG
YRKENYYDSQGRRVHEEHPRSTVTWKQNGIRHDTKNNRVRLSKGANHKEHPRAWEYILVEYETRPGVTVENLQQVRAVYDQQNERWELHLVCKDEIETPN
APGEETAGIDLGISNFAAVAYSTNEADLYPGNRLKQDGYYFLKEIANCDDSGGERAARLHHKWSERRTHFFHSLAKHIIQRCVENGVCRINVGDLEGVRE
DEHGESKNWGKHGNLDLHGWAFDRFTKILTYKAKVEGIEVVEVSERDTSKTCCICGREDDSQRVQRGLYVCEACDAAFNADVNGAENIRLNINDESNSES
APSLGGDRSTGWLAQPEVYLHSLSCGFQPQTEVVDCKP

Blast result :

Comments

ISNpe30 is 94% aa similar to ISNma19.

References

1] Pfeiffer F. (2015) Direct submission
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission to GenBanK.