ISHwa17

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AM180088 ND Haloquadratum walsbyi
Haloquadratum walsbyi DSM 16790
DNA section
IS Length : 2033 bp

Ends


Left end : CAGAGAAAGCAGCCAGAGTGCCTCGGGGCTTTGATATTGATGTGACCCCGAGGCTGAATGCCGTAAGCTCCAGGAGACGTGTTCAGTACGTTCGATATAC II struct. : Yes
Right end : GCCTCTGATACCGCTCAAGGGCACCATCCTGATGGTATCCGAGGCGTGTCCGACCAATCCACGGGAAGAAGCTCCGGGGCTTGACCCCGGAGCAGTTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GCATATGATGTCTAATTGCAGAACtcac
CAGTCGTATATCTATGGGGAATACtcac

DNA sequence

CAGAGAAAGCAGCCAGAGTGCCTCGGGGCTTTGATATTGATGTGACCCCGAGGCTGAATGCCGTAAGCTCCAGGAGACGTGTTCAGTACGTTCGATATAC
TGCTCAATCGTCTGGCTCGATACTTCACCAGCCGTTCCGATGTAGTATGATTCTTCCCAGAATCCCCCACCCCACAAGTGGTTTTCCAAGAGGTGTTCGT
GTTGCTGCCACATCTCTCTGGCTGTGATGCTCTTGATTTTTCGTGCAATCTCGCTTGTGGAATGTTTCGGATGTGCTGAAAGAAACAACTGAACGTGGTC
TGGCGAGATATGCAACGACAATATCTCGTAATTGTACTCGTCACACACCGCTCTGAACGATGTTTCTCACGACTGTTCTATCTCACCAAGCACTGAGCGG
CGGTACTTCCGACATTGCAAATCTCCGATTTGCAAGCAGTCAATCTCCGATTGACAACACCACACAAAGTGGTAGTTGATGTTATACACAGTGTGATTTG
ACCGTGTTTCGCCCATATCGGCTAGAGTCATGCCCAAAAGTTAAGCATAATTGTGTCTAAGTATATACTGTAGAGATGACCAAACGCCTCACCGTAGACT
TCGAGGACGATTTGTACAAGGAGTTCTCGAAGAAATGCATCGACGCTGAACGTCCAAAGTCAGAAGTCGTGCGCGAACTCGTCCAAGACTGGGTAACCGA
TCAAGAATGACGCAGACACAGGCTCTCACCAAGACGCTCGTGTTTCCGCTGGACGTGCAGAGTGGCAACGAGAGCCTGCTTCACGACGCCCGCTTGGAGT
GTCGCCGCGTGTTCACCGAGGTACTGCGCCTCAACTACGACGGGTGGGACTGGAATAGGATTGAGGATGTTGTTGAGCAGAACGCCGACCTCGTACAAAA
CACCGCACAGCGCATCATCGACAAAGCCTTCGAAGCACTCACGCAATACTACGACTACGACGACTGGGGCAGACCGTGGTACAAACACGAGACGTTCCCA
CTGCGGATGAACTACGGCGAAGGCTACAACCTCTTTCTCGAAGACGAAACAGTACGGTTCCGCATCAGTGCGAAACCGTACAACCATGTCAAAGGCGAAC
TGCGTGGCACACAAGACCAGTTTGACCTGCTCGCACAGGCAATCGAAGATGATGACTGGCACGTTGGTACTGCCGAAGCACTCTGCCGAAACGGACGCGA
AGAATTGCACGTTACCGTGACGAACGAGACAGCCGAAGTCGGGGCAAAAACAGCGGCAGAAACGGTCATCGGTGTCGATATTAACGAAGACTGCATTGCA
CTGGCGGCACTAACCGAACACGGCATTGAAGATTCAATTGTTATCGACTACCCAGAAATCAAGGAAGAACGACACCGGTACTTCACGATGCGGAAACGGA
TGCAAGAAGCCGGACAGACCGCGTTTAACGACGTGTTCCGCGACAAGGAACAACGGTTCGTTCACGACCAGCTACACACGGTGTCACGTCGCGTAGTCGA
GTGGATTCAACGGTTACGGTTCGACAGTCCTGTAATTGTCTTTGAAGACCTCACAGACATGAGAGACGACATTGAGTACGGGACTCGTATGAACCGGCGA
CTCCATTCATTACCGTTCGCCAAACTTCGGGATTTCATCACGTACAAAGCCGCATGGCACGGGATTCCGTCAGATGACGTTGACCCGGAGTACACCAGCC
AACAGTGTCCGGTCTGTGGACACACAGAACGTGCAAATCGCCACAAGAAGCGGTTCAAATGCCGTGAGTGCGAGCATCAAGACCACGCTGACCGTGGCGC
TGGTGTCAGTGTCGGGCAAAAGTGGCTGAGGAAGCAAAACAATCGAAATGTGCCTGCTCTCAACACACTGCCACAGGTGCGGAAGTGGGAGTTGCGACGG
CAGGCATCGGGGCCTGTGGACGGCCCGACCGTGGCCTCTGATACCGCTCAAGGGCACCATCCTGATGGTATCCGAGGCGTGTCCGACCAATCCACGGGAA
GAAGCTCCGGGGCTTGACCCCGGAGCAGTTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
444 bp148 aa51673-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MGETRSNHTVYNINYHFVWCCQSEIDCLQIGDLQCRKYRRSVLGEIEQS*ETSFRAVCDEYNYEILSLHISPDHVQLFLSAHPKHSTSEIARKIKSITAR
EMWQQHEHLLENHLWGGGFWEESYYIGTAGEVSSQTIEQYIERTEHVS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1311 bp436 aa7072017+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MTQTQALTKTLVFPLDVQSGNESLLHDARLECRRVFTEVLRLNYDGWDWNRIEDVVEQNADLVQNTAQRIIDKAFEALTQYYDYDDWGRPWYKHETFPLR
MNYGEGYNLFLEDETVRFRISAKPYNHVKGELRGTQDQFDLLAQAIEDDDWHVGTAEALCRNGREELHVTVTNETAEVGAKTAAETVIGVDINEDCIALA
ALTEHGIEDSIVIDYPEIKEERHRYFTMRKRMQEAGQTAFNDVFRDKEQRFVHDQLHTVSRRVVEWIQRLRFDSPVIVFEDLTDMRDDIEYGTRMNRRLH
SLPFAKLRDFITYKAAWHGIPSDDVDPEYTSQQCPVCGHTERANRHKKRFKCRECEHQDHADRGAGVSVGQKWLRKQNNRNVPALNTLPQVRKWELRRQA
SGPVDGPTVASDTAQGHHPDGIRGVSDQSTGRSSGA

 

Blast result :
Comments
ISHwa17 is 85% (TnpA : the transposase) and 94% (TnpB) aa similar to ISNph7.
The transposase is not active : there is a STOP codon in frame and the STOP codon is missing at the end of the sequence.
References
1] Bolhuis, H., Palm, P., Wende, A., Falb, M., Rampp, M., Rodriguez-Valera, F., Pfeiffer, F., and Oesterhelt, D. (2006)BMC Genomics 7, 169
2] Dyall-Smith,M.L., Pfeiffer,F., Klee,K., Palm,P., Gross,K., Schuster,S.C., Rampp, M., and Oesterhelt,D. (2011) PLoS One 6: e20968